Flag Tag Monoclonal Antibody

Datasheet

Code	CSB-MA000021M0m
Size	US$200
Image	Western Blot Positive WB detected in: 10ng Flag Tag fusion protein Flag Tag antibody at 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 35 kDa Observed band size: 35 kDa Western Blot Positive WB detected in: Flag Tag fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.625ng, 1.5625ng, 0.78ng All lanes: Flag Tag antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 35 kDa Observed band size: 35 kDa Western Blot Positive WB detected in: Flag Tag fusion protein 1, 2, 3, 4 at 50ng All lanes: Flag Tag antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 59.6, 36, 35, 42 kDa Observed band size: 59.6, 36, 35, 42 kDa Western Blot Positive WB detected in: Flag Tag fusion protein at 10ng All lanes: Company A, Company B, Company C, Company D, CSB-MA000021M0m antibody at 1:50000, 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000, 1:3200000, 1:6400000 Secondary Goat polyclonal to Mouse IgG at 1/50000 dilution Predicted band size: 35 kDa Observed band size: 35 kDa Western Blot Positive WB detected in: Flag Tag fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng, 1.5625ng, 0.78ng, 0.39ng All lanes: Company A, Company B, Company C, Company D, CSB-MA000021M0m antibody at 1:1000 Secondary Goat polyclonal to Mouse IgG at 1/50000 dilution Predicted band size: 35 kDa Observed band size: 35 kDa Western Blot Positive WB detected in: 5 different recombinant proteins with Flag Tag All lanes: Company A, Company B, Company C, Company D, CSB-MA000021M0m antibody at 1:5000 Secondary Goat polyclonal to Mouse IgG at 1/50000 dilution Immunoprecipitating Flag Tag in Transfected HEK-293 cell whole cell lysate Lane 1: Mouse control IgG instead of CSB-MA000021M0m in Transfected HEK-293 cell whole cell lysate Lane 2: CSB-MA000021M0m (1µl) + Transfected HEK-293 cell whole cell lysate (500µg) Lane 3: Transfected HEK-293 cell whole cell lysate (20µg) For western blotting, the blot was detected with CSB-MA000021M0m at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000 Immunoprecipitating Flag Tag in 293F transfected whole cell lysate Lane 1: Mouse control IgG instead of CSB-MA000021M0m in Flag Tag in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: Company A (1µl), Company B (1µl), Company C (1µl), Company D (1µl), CSB-MA000021M0m (1µl) + Flag Tag in 293F transfected whole cell lysate (500µg) Lane 3: Flag Tag in 293F transfected whole cell lysate (20µg) Immunofluorescence staining of 293T cells transfected with Flag tag by CSB-MA000021M0m at 1:300, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-mouse IgG(H+L). The image on the right is the 293T cells transfected without Flag tag.
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Product Details
Citations
Customer Reviews and Q&A

Product Details

Description

Flag Tag monoclonal antibody CSB-MA000021M0m was produced in the mouse immunized by using the DYKDDDDK(Flag) synthetic peptide conjugate to KLH as the immunogen. The Flag tag, a popular short peptide tag (DYKDDDDK), can be fused to the C- terminus or N-terminus of the protein. It is used in recombinant DNA technology and can be used for affinity chromatography and can also be used with other affinity tags.
This Flag Tag Monoclonal Antibody was tested in the WB, IF, IP and ELISA applications. The non-conjugated IgG2a got purified by protein G and reached up to 95% in purity. Flag antibodies can be used to detect and express the expression of Flag-tagged fusion proteins, intracellular localization, and to purify, qualitatively or quantitatively detect Flag fusion-expressed proteins. It doesn’t have species restricted.

Alternative Names

DDK tag, DYKDDDDK, Flag, Flag Tag

Raised in

Mouse

Species Reactivity

All

Immunogen

DYKDDDDK(Flag) synthetic peptide conjugate to KLH

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

IgG2a

Clone No.

4G5C8

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IF, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:10000
IF	1:100-1:300
IP	0.5ul-2ul

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

Stomatin modulates adipogenesis through the ERK pathway and regulates fatty acid uptake and lipid droplet growth SC Wu,Nature communications,2022
Galectin‐1–RNA interaction map reveals potential regulatory roles in angiogenesis J Wei,FEBS letters,2021
The role of the cytoplasmic tail of the influenza virus M2 protein in viral morphology and replication Badham M, et al,University of Kent,2018

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 2 reviews

Submit a Review here

Applications : MASS SPECTROSCOPY

Sample type: Human HEK-293T cells

Review: Cells were lysed and immunoprecipitated using monoclonal IgG2b anti-Flag antibody.

By Anonymous

Applications : Western Blot (WB)

Review: Cultured cells were washed twice with cold PBS, then lysed using RIPA lysis buffer. In western blotting assay, anti-Flag was applied from CUSABIO.

By Anonymous

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