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Flag Tag monoclonal antibody CSB-MA000021M0m was produced in the mouse immunized by using the DYKDDDDK(Flag) synthetic peptide conjugate to KLH as the immunogen. The Flag tag, a popular short peptide tag (DYKDDDDK), can be fused to the C- terminus or N-terminus of the protein. It is used in recombinant DNA technology and can be used for affinity chromatography and can also be used with other affinity tags.
This Flag Tag Monoclonal Antibody was tested in the WB, IF, IP and ELISA applications. The non-conjugated IgG2a got purified by protein G and reached up to 95% in purity. Flag antibodies can be used to detect and express the expression of Flag-tagged fusion proteins, intracellular localization, and to purify, qualitatively or quantitatively detect Flag fusion-expressed proteins. It doesn’t have species restricted.
DDK tag, DYKDDDDK, Flag, Flag Tag
|Immunogen||DYKDDDDK(Flag) synthetic peptide conjugate to KLH|
|Purification Method||>95%, Protein G purified|
|Concentration||It differs from different batches. Please contact us to confirm it.|
|Buffer||Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
|Tested Applications||ELISA, WB, IF, IP|
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
|Troubleshooting and FAQs||Antibody FAQs|
|Storage||Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.|
|Lead Time||Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.|
Applications : MASS SPECTROSCOPY
Sample type: Human HEK-293T cells
Review: Cells were lysed and immunoprecipitated using monoclonal IgG2b anti-Flag antibody.
Applications : Western Blot (WB)
Review: Cultured cells were washed twice with cold PBS, then lysed using RIPA lysis buffer. In western blotting assay, anti-Flag was applied from CUSABIO.