Recombinant Chicken Zona pellucida sperm-binding protein 3 (ZP3)

Code CSB-CF027121CH
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
Uniprot No.
Alternative Names
ZP3; ZPC; Zona pellucida sperm-binding protein 3; Sperm receptor; Zona pellucida C protein; Zona pellucida glycoprotein 3; Zp-3
Gallus gallus (Chicken)
Expression Region
Target Protein Sequence
Protein Length
full length protein
Tag Info
The following tags are available.
N-terminal His-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Please contact us to get it.

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Target Background

Component of the zona pellucida, which mediates species-specific sperm binding. Directly binds to sperm. Important for egg fertilization.
Gene References into Functions
  1. chicken ZP3 was separated into four major and distinct isoforms with different pI in 2D-PAGE. PMID: 22776203
  2. Crystal structure of an avian homolog of ZP3 at 2.0 A resolution. The structure unveils the fold of a complete ZP domain module in a homodimeric arrangement required for secretion and reveals how EHP prevents premature incorporation of ZP3 into the ZP. PMID: 20970175
  3. ZP1, in addition to ZPC, plays a major role in the initial interactions between sperm and egg. PMID: 15115720
  4. Chick ZP3 is secreted to the apical aspect of chicken granulosa cells towards the perivitelline matrix. PMID: 9780332
  5. Involvement in the formation of quail zona pellucida PMID: 15503149

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Subcellular Location
[Processed zona pellucida sperm-binding protein 3]: Secreted, extracellular space, extracellular matrix.; Cell membrane; Single-pass type I membrane protein.
Protein Families
ZP domain family, ZPC subfamily
Tissue Specificity
Detected in the ovarian perivitteline layer. Detected in granulosa cells in ovarian follicle (at protein level). Detected in granulosa cells in ovarian follicle.
Database Links
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