Recombinant Drosophila melanogaster Protein wntless (wls)

Code CSB-CF850199DLU
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Source in vitro E.coli expression system
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Product Details

Target Names
wls
Uniprot No.
Alternative Names
wls; evi; srt; CG6210; Protein wntless; Evenness interrupted; Sprinter
Species
Drosophila melanogaster (Fruit fly)
Expression Region
36-594
Target Protein Sequence
APLPAGHVTVLGSLCREDHARQNDTSFLLYSRGAGACIPVTREEVEQDSTKMANELVHVF QMPLPRDLRDLDYSRWQQNLIGVLQVEFGYDSSSELREPPRELQLTIDMRLAYRNKGDPD NGWKLYAHGVEHRYLDCVTSHVGPTETLYSCDMIPLFELGALHHSFYLLNLRFPLDTPSQ MNLQFGHMHDLTLTAIHQNGGFTQIWLLLKTMLFPFVVGIMIWFWRRVHLLQRSPALLEY MLIYLGAALTFLNLPLEYLSLVYEMPYMLLLSDIRQGIFYAMLLTFWLVFAGEHMLIQDA PNKSTIRSRYWKHLSAVVVGCISLFVFDICERGVQLRNPFYSIWTTPLGAKVAMTFIVLA GVSAAIYFLFLCYMIWKVFRNIGDKRTSLPSMSQARRLHYEVPLDQKVEDWAGIVYFYTK AFFFQLHKANESKGLIYRFKFLMLATLVCAALTVAGFIMGQMAEGQWDWNDNVAIQPTSA FLTGVYGMWNIYIFALLILYAPSHKQWPTMHHSDETTQSNENIVASAASEEIEFSHLPSD SNPSEISSLTSFTRKVAFD
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
A segment polarity gene required for wingless (wg)-dependent patterning processes, acting in both wg-sending cells and wg-target cells. In non-neuronal cells wls directs wg secretion. The wls traffic loop encompasses the Golgi, the cell surface, an endocytic compartment and a retrograde route leading back to the Golgi, and involves clathrin-mediated endocytosis and the retromer complex (a conserved protein complex consisting of Vps35 and Vps26). In neuronal cells (the larval motorneuron NMJ), the wg signal moves across the synapse via the release of wls-containing exosome-like vesicles. Postsynaptic wls is required for the trafficking of fz2 through the fz2-interacting protein Grip.
Gene References into Functions
  1. The uncharacterized Drosophila protein Evi5 was identified as an essential membrane trafficking regulator, and the molecular mechanism by which Evi5 regulates BC migration was described. PMID: 22778279
  2. Syx1A, Rab11, and its effector Myosin5 were required for proper Evi vesicle release. PMID: 22437826
  3. we propose that Wntless represents an ancient partner for Wnts dedicated to promoting their secretion into the extracellular milieu. PMID: 16678095
  4. Recent studies have identified Wntless (Wls) and the retromer complex as essential components involved in Wnt signaling. PMID: 18160348
  5. Wg, clathrin-mediated endocytosis and retromer sustain a Wls traffic loop from the Golgi to the plasma membrane and back to the Golgi, thereby enabling Wls to direct Wnt secretion. PMID: 18193032
  6. These findings uncover a previously unknown cellular mechanism by which a secreted Wnt is transported across synapses by Evi-containing vesicles and reveal trafficking functions of Evi in both the Wnt-producing and the Wnt-receiving cells. PMID: 19837038

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Subcellular Location
Cell junction, synapse, presynaptic cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Endosome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Note=In non-neuronal cells, wls binds to wg in the Golgi and accompanies it to the plasma membrane where the two proteins dissociate. Wg is secreted and wls is then internalized and returns to the Golgi apparatus in a retromer-dependent manner. Wls and wg colocalize in the Golgi apparatus in wg-producing cells, and reduced expression is seen in non-producing cells. Endoplasmic reticulum expression is unchanged in wg-producing versus non-producing cells. In neuronal cells, wls is localized both pre- and postsynaptically and is transferred trans-synaptically from the pre- to the postsynaptic compartment.
Protein Families
Wntless family
Tissue Specificity
Ubiquitously expressed in the wing imaginal disk, increased expression is observed in a stripe at the dorso-ventral boundary and other regions of the wing disk that express wg. Also expresses in the leg imaginal disk. During larval development, expression
Database Links

KEGG: dme:Dmel_CG6210

STRING: 7227.FBpp0075947

UniGene: Dm.7728

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