Recombinant Human Blood group Rh(D) polypeptide(RHD)

Code CSB-CF019677HU(A4)
Size US$3570
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 85% as determined by SDS-PAGE.
Target Names RHD
Uniprot No. Q02161
Research Area Cardiovascular
Alternative Names Blood group Rh(D) polypeptide; Blood group--Rhesus system D polypeptide; CD240D; D antigen (DCS); DIIIc; MGC165007; RH; Rh blood group antigen Evans; Rh blood group D antigen; Rh polypeptide 2; RH30; Rh4; RHCED; Rhd; RHD_HUMAN; RhDCw; RHDel; RHDVA(TT); Rhesus blood group D antigen allele DIII type 7; Rhesus D antigen; Rhesus system D polypeptide; RhII; RhK562-II; RhPI; RHPII; RHXIII
Species Homo sapiens (Human)
Source in vitro E.coli expression system
Expression Region 2-417aa
Target Protein Sequence SSKYPRSVRRCLPLWALTLEAALILLFYFFTHYDASLEDQKGLVASYQVGQDLTVMAAIGLGFLTSSFRRHSWSSVAFNLFMLALGVQWAILLDGFLSQFPSGKVVITLFSIRLATMSALSVLISVDAVLGKVNLAQLVVMVLVEVTALGNLRMVISNIFNTDYHMNMMHIYVFAAYFGLSVAWCLPKPLPEGTEDKDQTATIPSLSAMLGALFLWMFWPSFNSALLRSPIERKNAVFNTYYAVAVSVVTAISGSSLAHPQGKISKTYVHSAVLAGGVAVGTSCHLIPSPWLAMVLGLVAGLISVGGAKYLPGCCNRVLGIPHSSIMGYNFSLLGLLGEIIYIVLLVLDTVGAGNGMIGFQVLLSIGELSLAIVIALMSGLLTGLLLNLKIWKAPHEAKYFDDQVFWKFPHLAVGF
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 47.9 kDa
Protein Length Full Length of Mature Protein
Tag Info C-terminal 10xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function May be part of an oligomeric complex which is likely to have a transport or channel function in the erythrocyte membrane.
Gene References into Functions
  1. 4 novel RHD alleles, each characterized by a single nucleotide substitution were identified. RHD*67T, RHD*173T, and RHD*579C give rise to a weak D phenotypical expression. Their corresponding amino acid changes are predicted to be located in the membrane-spanning or intracellular domains of the RhD protein. RHD*482G is the 4th substitution. PMID: 29052223
  2. Extensive studies show that the RHD*1227A is the most prevalent DEL allele in East Asian populations and may have confounded the initial molecular studies. PMID: 29214630
  3. The most prevalent DEL allele was RHD*DEL1 (c.1227G>A), which is proven to be immunogenic. A high frequency of RHD*Psi was detected in the donors with nondeleted RHD alleles (31%), far superior to the frequency of RHD variant alleles (15.5%). PMID: 29193119
  4. Absence of the whole RHD gene is common among RhD negative blood donors from Qingdao region, and there are rich genetic polymorphisms for this locus PMID: 29188626
  5. The RHD 1227G>A mutation contributes to the molecular basis of Del phenotype in the Taiwanese population. The point mutation results in aberrant frame shift or exon deletion transcripts and generates D protein with weak antigen presenting function. PMID: 26774048
  6. In this mixed Brazilian population, the most frequent weak D types were 1, 4, 3 and 2 (frequencies of 4.35%, 2.32%, 1.46% and 0.29%, respectively; total of 8.41%) and partial D was found in 2.90% of samples carrying the RHD gene. For samples with inconclusive RhD typing, 53.33% of them presented weak and partial RHD, and 43.75% had concomitantly more than one RHD variant PMID: 27184292
  7. sequence comparisons revealed high sequence similarity between Patr_RHbeta and Hosa_RHCE, while the chimpanzee Rh gene closest to Hosa_RHD was not Patr_RHa but rather Patr_RHy PMID: 26872772
  8. Six weak D types in the Russian Federation: the most common type 3 (49.2%) and type 1 (28.6%), type 2 (14.3), type 15 (4.8%), type 4.2 (DAR) (1.6%) and type 6 (1.6%). PMID: 27459619
  9. The frequency of RhD negative homozygosity in the Cypriot population was estimated to be 7.2%, while the frequencies of RHD hemizygosity and RhD positive homozygosity was calculated to be 39.2 and 53.6%, respectively. PMID: 27036548
  10. Occurrence of partial RhD alleles in the Tunisian population. PMID: 26482434
  11. Reduced expression of D antigen is caused not only by missense mutation of the RHD gene, but also by silent mutation that may affect splicing. PMID: 26340140
  12. Loss of heterozygosity of RhD gene on chromosome 1p in acute myeloid leukemia. PMID: 25495174
  13. The data indicate that partial DEL women appear at risk of alloimmunization to the D antigen. PMID: 26033335
  14. Weak D type 4.0 appears to be the most prevalent weak D in our population. However, all samples must be sequenced in order to determine the exact subtype of weak D type 4, since weak D type 4.2 has considerable clinical importance PMID: 25369614
  15. Paternal RHD zygosity determination in Tunisians: evaluation of three molecular tests. PMID: 24960665
  16. Serologic findings of RhD alleles in Egyptians and their clinical implications. PMID: 25219636
  17. Despite the enormous diversity of RHD alleles, first-line weak D genotyping was remarkably informative, allowing for rapid classification of most samples with conspicuous RhD phenotype in Flanders, Belgium. PMID: 25413499
  18. Splicing is altered in RHD*weak D Type 2 allele, a rare variant most commonly found in Caucasians; RHD including the full-length Exon 9 is transcribed in the presence of the c.1227G>A substitution frequently carried by Asians with DEL phenotype. PMID: 25808592
  19. Among all donors 89.00% and 10.86% were D-positive and D-negative, respectively, while 0.14% (n=55) of the donors were found to be weak D-positive. PMID: 24960662
  20. The frequency of D variants detected by IAT allele RHD(M295I) was 1:272 in D negative donors. Obviously, DEL phenotype is more common in some parts of European population than initially thought. PMID: 24556127
  21. New RHD variant alleles. PMID: 25179760
  22. Currently, it seems to be difficult to observe any new RHD alleles in the Han Chinese population. D prediction in this population is easier because popular alleles are dominant, accounting for about 99.80% of alleles in D-negative people. PMID: 24333088
  23. In Han Chinese people with weak D serotyping, 8 weak D and 4 partial D alleles were found. 3 new weak D alleles (RHD weak D 95A, 779G, and 670G) and one new partial D allele (RHD130-132 del TCT) were identified. PMID: 25070883
  24. DEL/weak D-associated RHD alleles were found in 2.17% of Australian D-, C+ and/or E+ blood donors. PMID: 24894016
  25. RHD alleles and D antigen density among serologically D- C+ Brazilian blood donors. PMID: 24267268
  26. In this study, D antigen density on the erythrocyte surface of DEL individuals carrying the RHD1227A allele was extremely low, there being only very few antigenic molecules per cell, but the D antigen epitopes were grossly complete. PMID: 24333082
  27. The prevalence of D-/RHD+ samples is higher than that observed in Europeans. More than 50% of the RHD alleles found were represented by RHDpsi and RHD-CE-D(s) showing the African contribution to the genetic pool of the admixed population analyzed. PMID: 24819281
  28. A method of genotyping has been developed in the laboratory. genotyping results of 200 pregnant women have been compared with RH1 phenotype at birth. PMID: 24559796
  29. We conclude that noninvasive fetal RHD genotyping from maternal blood provides accurate results and suggests its viability as a clinical tool for the management of RhD-negative pregnant women in an admixed population. PMID: 24615044
  30. Two molecular polymorphisms to detect the (C)ce(s) type 1 haplotype. PMID: 24333080
  31. This study analyzes the phenotype and frequency of RhD and tetanus toxoid specific memory B cells in limiting dilution culture. PMID: 24965774
  32. Data indicate that non-invasive prenatal testing of cell-free fetal DNA (cffDNA) in maternal plasma can predict the fetal RhD type in D negative pregnant women. PMID: 24204719
  33. DIV alleles arose from at least two independent evolutionary events. DIV Type 1.0 with DIVa phenotype belongs to the oldest extant human RHD alleles. DIV Type 2 to Type 5 with DIVb phenotype arose from more recent gene conversions. PMID: 23461862
  34. RHD*DARA and RHD*DAR2 are the same allele. Furthermore, the alleles RHD*DAR1.2 and RHD*DAR1.3 both exist; however, the silent mutation 957G>A (V319) showed no influence on the RhD phenotype. PMID: 23902153
  35. All novel weak D types expressed all tested D epitopes. PMID: 23550956
  36. Only 0.2% of D- Polish donors carry some fragments of the RHD gene; all of them were C or E+. Almost 60% of the detected RHD alleles may be potentially immunogenic when transfused to a D- recipient. PMID: 23634715
  37. This study is the first to describe weak D types caused by intronic variations near the splice sites in the RHD gene, which is supported by the genotyping results combined with serologic profiles and bioinformatics analysis. PMID: 23216299
  38. RHD variants were identified in 91.6% of the 430 studied samples. Two of the nine previously undescribed variants, c.335G>T and c.939G>A, were found to cause aberrant mRNA splicing by means of a splicing minigene assay. PMID: 23228153
  39. Hemizygous RHD subjects demonstrated significantly higher platelet increases and peak platelet counts than homozygous RHD subjects. PMID: 23712954
  40. The RHD*weak 4.3 allele with markedly reduced antigen D expression was shown to be associated with an altered RHCE gene formation leading to the expression of C(X) and VS. PMID: 22288371
  41. modulates the influence not only of latent toxoplasmosis, but also of at least two other potentially detrimental factors, age and smoking, on human behavior and physiology. PMID: 23209579
  42. RHD*DIVa and RHCE*ceTI almost always, but not invariably, travel together. This haplotype is found in people of African ancestry and the red blood cells can demonstrate aberrant reactivity with anti-C. PMID: 22804620
  43. RHD*DOL2, like RHD*DOL1, encodes a partial D antigen and the low-prevalence antigen DAK. PMID: 22738288
  44. The use of cell-free fetal DNA in prenatal noninvasive early detection of fetal RhD status and gender by real-time PCR is highly sensitive and accurate as early as the 11th week of gestation for RhD status and the 7th week of gestation for fetal sex. PMID: 21488716
  45. This deletion appears to represent not only the first large deletion associated with weak D but also the weakest of weak D alleles so far reported. PMID: 22420867
  46. Characterization of novel RHD alleles. PMID: 22320258
  47. The RHD genotyping proved to be a necessary tool to characterise RHD alleles in donors phenotyped as D- or weak D to increase the transfusion safety in highly racial mixed population. PMID: 22211984
  48. RHD homozygotes had nearly twice as many D antigen sites as hemizygotes. Expression of c or E antigens was associated with increased RBC D antigen expression, but presence of C or e antigens reduced expression. PMID: 22121029
  49. Anti-D investigations in individuals expressing weak D Type 1 or weak D Type 2 PMID: 21658048
  50. Distribution of weak D types in the Croatian population. PMID: 21269342
  51. next-generation sequencing offers a new development for high-throughput and clonal sequencing for molecular RHD genotyping. PMID: 21133932
  52. Molecular bases of unexpressed RHD alleles in Chinese D- persons. PMID: 19392776
  53. 4 novel RHD alleles were identified: RHD(S256P), RHD(L390L), RHD(F410V), and RHD(IVS4-2a>g). PMID: 20723165
  54. This QF-PCR method accurately determines RHD zygosity and will help predict the risk that a fetus will inherit RHD. PMID: 21072752
  55. These results indicate that RhD phenotype might influence not only the effect of toxoplasmosis but also the effect of aging on specific personality traits. PMID: 20608477
  56. combined haplotype of 1227G>A and IVS7 923C>T alleles was apparent in >95% DEL Chinese individuals. RHD1227A mutation significantly increased aberrant mRNA splicing, producing a hybrid RHD mRNA lacking exon PMID: 20188798
  57. four RhD protein varients possessed amino acid substititions at postions 114 and 122 PMID: 20233350
  58. RHD genes in population of Fujian Province are polymorphic. PMID: 18426681
  59. Epitope mapping of four monoclonal antibodies specific for the human RhD antigen PMID: 11716963
  60. characterization of 5 new RHD alleles, dubbed DAU-0 to DAU-4, that share a T379M substitution and occurr in a cDe haplotype. PMID: 12070041
  61. Molecular analysis of Hor+, Mol+ variants revealed a hybrid gene structure RHCe-D(5)-Ce, in which exon 5 of RHCE (RHCe allele) was replaced by exon 5 of RHD (the so-called RHCeVA allele resulting in several AA alterations in the external loop 4 CeVA. PMID: 12084172
  62. RHD maternal-fetal genotype incompatibility increases schizophrenia susceptibility PMID: 12439825
  63. evaluation of posttransplant sensitization in blood this group-negative renal transplantation recipients PMID: 12493401
  64. Fetal RHD was detected in all 23 mothers with RhD pos child. PMID: 15112469
  65. CD5+ CLL cells are the most effective cell type in processing and presenting purified Rh protein to autoreactive T helper cells. PMID: 15284121
  66. Five new RHD alleles were characterized carrying 399G > T, 680T > C, 833G > A, 851C > T, and 1015G > A, respectively. PMID: 15318849
  67. Review. The genetic, structural, and immunologic features of RHD are reviewed. PMID: 15373666
  68. 6 new alleles are described: A1226T, T667G,G697C,T1136C,G998A,and C674T. PMID: 16181204
  69. 2 new weak D types (31 & 32)were found, C17T & A1121T, coding for amino acid exchanges in predicted intracellular RhD polypeptide stretches, with antigen densities of approximately 130 & 50 D sites per red blood cell, respectively. PMID: 16181207
  70. The transcripts with exon 9, exons 8 and 9, exons 7 and 9, and exons 7-9 spliced out compared normal RhD mRNA. PMID: 16510313
  71. By describing the RHD(F223V) (602C>G) and RHD(T201R, F223V) (602C>G and 667T>G) alleles formal proof is given for the origin of the non-Eurasian cluster. PMID: 16584437
  72. A novel pattern of RhD exon amplification was detected, characterized, and named (DVI type 4). This novel structure is the most frequent cause of DVI in Spain. PMID: 16584438
  73. RHD 1227A allele is an important genetic marker of Rh DEL phenotype; RHD 1227A is recessive to normal RHD allele and dominant to RHd allele; RHD 1227A allele is an ancestral, but not a spontaneously mutated allele. PMID: 17029203
  74. the mRNA splicing mechanism of RHD gene was very complex, and nine novel alternative splicing isoforms were identified PMID: 17035176
  75. In this [Portuguese] population, 1.3% of the serologic RhD-negative women have an RHD-positive allele. PMID: 17497744
  76. DCS-1, DCS-2, and DFV carry amino acid substitutions at the extracellular vestibule, visualized by 3-dimensional modeling. PMID: 17900276
  77. The investigators analyzed the admixture genetic components of the Rh blood group in two socioeconomic groups in Venezuela. PMID: 18027815
  78. Anti-Rh(D) may have application for islet cell proliferation in diabetes mellitus treatment for Rh(D)-positive subjects. PMID: 18544617
  79. relatively high perioperative transfusion needs of D- orthotopic liver transplantation recipients can be safely met with D+ components with very low risk of inducing anti-D with the newer, mycophenolate mofetil-centered immune suppression regimen PMID: 19055536
  80. 74 blood donor samples carrying weak D and DEL phenotypes with the potential of causing secondary immunizations in recipients were reclassified as D+. PMID: 19170995
  81. variant sequences in Intron 7 of Rh blood group D antigen nucleotides AG-GT at both sides of the segment may be related to this molecular even as AG-GT may cut intron 7 with its normal splicing site (GT-AG) into two "new introns" PMID: 19364677
  82. The proximal RHD/RHCE promoter regions contain cis-regulatory binding motifs and an internal sequence-dependent region that together regulate transcription suggesting that this region may be relevant in the weak expression of RhD PMID: 19374729
  83. More precise determination of D antigen helps to reveal the Rhesus factor PMID: 19388479
  84. Molecular basis of D antigenic epitopes PMID: 10590042

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Subcellular Location Membrane, Multi-pass membrane protein
Protein Families Ammonium transporter (TC 2.A.49) family, Rh subfamily
Tissue Specificity Restricted to tissues or cell lines expressing erythroid characters.
Database Links

HGNC: 10009

OMIM: 111680

KEGG: hsa:6007

STRING: 9606.ENSP00000331871

UniGene: Hs.449968

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