Recombinant Human Fatty acid desaturase 1(FADS1)

Code CSB-CF007959HU
Size Pls inquire other sizes
Source in vitro E.coli expression system
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Product Details

Target Names FADS1
Uniprot No. O60427
Alternative Names D5D; Delta 5 desaturase; Delta 5 fatty acid desaturase; Delta(5) desaturase; Delta(5) fatty acid desaturase; Delta-5 desaturase; Fads1; FADS1_HUMAN; FADS6; FADSD5; Fatty acid desaturase 1; FLJ38956; Linoleoyl CoA desaturase (delta 6 desaturase) like 1; LLCDL1; TU12
Species Homo sapiens (Human)
Expression Region 1-501
Target Protein Sequence MGTRAARPAGLPCGAENPARRRLALGARQQIHSWSPRTPSTRLTAPAGPARGVARPAMAPDPVAAETAAQGPTPRYFTWDEVAQRSGCEERWLVIDRKVYNISEFTRRHPGGSRVISHYAGQDATDPFVAFHINKGLVKKYMNSLLIGELSPEQPSFEPTKNKELTDEFRELRATVERMGLMKANHVFFLLYLLHILLLDGAAWLTLWVFGTSFLPFLLCAVLLSAVQAQAGWLQHDFGHLSVFSTSKWNHLLHHFVIGHLKGAPASWWNHMHFQHHAKPNCFRKDPDINMHPFFFALGKILSVELGKQKKKYMPYNHQHKYFFLIGPPALLPLYFQWYIFYFVIQRKKWVDLAWMITFYVRFFLTYVPLLGLKAFLGLFFIVRFLESNWFVWVTQMNHIPMHIDHDRNMDWVSTQLQATCNVHKSAFNDWFSGHLNFQIEHHLFPTMPRHNYHKVAPLVQSLCAKHGIEYQSKPLLSAFADIIHSLKESGQLWLDAYLHQ
Protein Length full length protein
Tag Info The following tags are available.
N-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Isoform 2 does not exhibit any catalytic activity toward 20
Gene References into Functions
  1. FADS1 role in the oleic acid metabolism PMID: 29413358
  2. review identified that FADS polymorphism may alter plasma fatty acid composition and play a protective role in the development of type 2 diabetes PMID: 29899246
  3. FADS1/2 SNPs affect dietary fatty acid metabolism in centrally obese postmenopausal Polish women. PMID: 30103441
  4. The results of this population-based study provide evidence for a relationship between lipid regulatory gene polymorphisms including GCKR (rs780094), GCKR (rs1260333), FADS (rs174547), and MLXIPL (rs3812316) with dyslipidemia in an Iranian population. PMID: 29858861
  5. An association was found between the rs174550 FADS1 polymorphism and gestational diabetes mellitus risk. PMID: 28359772
  6. commonly overexpressed COX in cancer (~90% of colon cancer patients) can be taken advantage to suppress cell growth by knocking down delta-5-desaturase (D5D, a key enzyme that converts DGLA to arachidonic acid). In addition, D5D knockdown along with DGLA supplement may enhance the efficacy of chemotherapeutic drugs PMID: 27101738
  7. In minor allele carriers of FADS1 and FADS2, plasma arachidonic acid (ARA) content was elevated only at the highest level of ARA consumed in infant formula. ARA level in plasma is reduced by low ARA consumption and by minor alleles in FADS in infants fed ARA-containing formula. PMID: 27276431
  8. D5D knockdown in conjunction with dihomo-gamma-linolenic acid treatment can also be used to inhibit growth of pancreatic cancer cells via p53 independent pathway. PMID: 27368132
  9. Our results showed that knockdown of delta-5-desaturase along with DGLA supplement not only significantly inhibited cell migration, but also improved the efficacies of 5-flurouracil and gemcitabine, two frontline chemotherapy drugs currently used in the treatment of colon and pancreatic cancer. PMID: 28157665
  10. The majority of CpG sites (117 out of 136, 86%) exhibited high levels of methylation with the greatest variability observed at three key regulatory regions-the promoter regions for FADS1 and FADS2 and a putative enhancer site between the two genes. PMID: 28957329
  11. FADS1 rs174547 and FADS2 rs2727270 genotypes were significantly correlated with decreased HDL-C concentrations, and D5D /D6D activities as estimated as 20:4(n-6)/20:3 (n-6) and 18:3 (n-6)/18:2 (n-6) in a linear pattern in patients with type 2 diabetes PMID: 28555039
  12. Results indicate that genetic variation in the FADS1 gene, rs174546, influences blood pressure via arachidonic acid and body mass index. Thus, polymorphisms with an impact on the delta-5 desaturase activity may play a role for the blood pressure level mediated through polyunsaturated fatty acids and body mass index. PMID: 28732058
  13. FADS1 rs174549 polymorphism and fish consumption may be protective factors for oral cancer, with a gene-diet multiplicative interaction PMID: 28178666
  14. The derived allele of rs174557, which is the common variant in most populations, diminishes binding of PATZ1, a transcription factor conferring allele-specific downregulation of FADS1. PMID: 27932482
  15. a 2-locus constructed haplotype (rs174547-rs174553) affected gamma-linoleic acid and arachidonic acid concentration in breast milk of Chinese women PMID: 27269715
  16. This study showed associations between FADS 1/2 SNPs and cognitive performance. rs1535 minor allele homozygosity and rs174448 major allele carriage associated with improved cognitive performance in 8- to 11-y-old boys but not in girls, thereby counteracting existing sex differences. PMID: 28515069
  17. we determined rs66698963 genotype frequencies from 234 individuals of a primarily vegetarian Indian population and 311 individuals from the US. A much higher I/I genotype frequency was found in Indians (68%) than in the US (18%). A much higher I/I genotype frequency was found in Indians (68%) than in the US (18%). A global I/I genotype of 70% in South Asians, 53% in Africans, 29% in East Asians, and 17% in Europeans PMID: 27188529
  18. An association of FADS1 rs174547 with the fatty acid composition of plasma phospholipids was identified among this Chinese adult population. PMID: 28359317
  19. rs174549 polymorphism is favorable factor in predicting oral cancer progression-free survival in response to chemoradiotherapy PMID: 27542551
  20. the single nucleotide polymorphism rs174547 (T/C) of the fatty acid desaturase-1 gene, FADS1, is associated with changes in arachidonic acid compositions of the blood of elderly Japanese PMID: 26869086
  21. Our results demonstrate that FADS1-FADS2 are susceptibility genes for PCOS. PMID: 26879377
  22. FADS1 rs174546 single nucleotide polymorphism correlation with fatty acids profiles in blood PMID: 26346302
  23. Single nucleotide polymorphisms in FADS gene (particularly rs174537) associate with plasma fatty acids and desaturase levels in patients with both type 2 diabetes and coronary artery disease. PMID: 27004414
  24. FADS1 variants modulate the relationship between marine fatty acid intake and circulating levels of long-chain omega-3 fatty acids. PMID: 26501394
  25. No associations were observed between any single-nucleotide polymorphisms and major depressive disorder diagnosis or attempted suicide status. PMID: 26513616
  26. polymorphisms in the FADS gene cluster were nominally associated with reduced risk of developing atopic eczema PMID: 26633493
  27. genetic association study in population of pregnant women in Republic of Seychelles: SNPs in FADS1/FADS2 are associated with maternal arachidonic acid status; a subtle association exists with neurodevelopment in this high fish-eating population. [REVIEW] PMID: 26474818
  28. Suggest that the FADS1 rs174546 SNP and the FADS2 rs174601 SNP are associated with the risk of coronary artery disease and ischemic stroke, and are likely to influence serum lipid levels. PMID: 26261632
  29. genome-wide association study in population of mother/infant dyads in Denmark: Data suggest SNPs in maternal FADS1 are associated with (1) lower breast milk polyunsaturated fatty acid and (2) higher production of interleukins by infant T-lymphocytes. PMID: 26283408
  30. Dietary fatty acids modulate associations between FADS1 genetic variants and circulating fatty acids in plasma and erythrocyte membranes. PMID: 25626431
  31. Decreased Expression of FADS1 is associated with Esophageal Squamous Cell Carcinoma. PMID: 26163647
  32. High dietary n-3 LCPUFA intakes could negate the unfavorable effect of genetic variation in FADS1 on CAD in middle-aged and elderly Chinese population. PMID: 25849351
  33. Data suggest that down-regulation of FADS1 (delta-5 fatty acid desaturase) and up-regulation of FADS2 (delta-6 fatty acid desaturase) in obesity leads to altered long-chain polyunsaturated fatty acid nutritional status. [META-ANALYSIS, REVIEW] PMID: 25828602
  34. In northern Han Chinese people, the minor allele (T) of rs174616 in the FADS1-FADS2 gene cluster is associated with a decreased conversion rate of Linoleic acid to Arachidonic acid, which may contribute to decreased reduced risk of developing T2 diabetes. PMID: 25981324
  35. Study shows that docosahexaenoic acid (DHA) and arachidonic acid (ARA) levels were lower in pregnant women homozygous for FADS1 rs174533 but after DHA supplementation, ARA level decreases in women homozygous for both FADS1rs174533 and FADS2rs1747575. PMID: 25500337
  36. Fads1 and Fads2 gene expression was reduced by EPA and AA, but not ALA or LA PMID: 25755223
  37. rare alleles of SNPs significantly associated with the accumulation of multiple long-chain fatty acids in the liver PMID: 25123259
  38. examined the impact of a highly influential FADS SNP, rs174537 on leukotriene, HETE, prostaglandin, and thromboxane biosynthesis in stimulated whole blood PMID: 24962583
  39. genetic association study in population of children in Denmark: Data suggest that SNPs in FADS1 (rs1535, rs174448, rs174575) are associated with developmental psychomotor disorders at 30 months of age. PMID: 25080457
  40. Minor allele homozygotes and heterozygotes of rs174575 and rs174537 had lower PL 20:4 omega-6 levels in healthy individuals. PMID: 24985009
  41. maternal rs174556 FADS SNP modified the associations of maternal docosahexaenoic acid (DHA) intake in pregnancy with pregnancy duration and child birth weight PMID: 24991040
  42. These results suggest that genetic variation at the FADS locus can influence an individual's response to fish oil supplements. PMID: 24936800
  43. we found weak, but not convincing, evidence of effect modification by genetic variation in FADS1 on the associations between polyunsaturated fatty acid intakes and cardiovascular disease risk. PMID: 25008580
  44. Recent genome-wide association studies showed that FADS1/FADS2 genetic locus, beyond being the main determinant of PUFA, was strongly associated with plasma lipids and glucose metabolism. PMID: 25038994
  45. ALA intake was significantly more protective for IA in the presence of an increasing number of minor alleles at FADS1 PMID: 24240437
  46. Single-nucleotide polymorphisms in the FADS1 gene is associated with lower arachidonic acid concentrations in the colon. PMID: 24022589
  47. Changes in the mRNA-expression levels of FADS1 and 2 directly affect blood DGLA levels and D6D activity. This study suggests that lower mRNA-expressions of FADS2 and ELOVL5 are associated with higher risk of atopic eczema in young children. PMID: 24167612
  48. Weak evidence suggests that SNPs in FADS1/2 may influence high sensitivity C-reactive protein levels in young adults. PMID: 23867726
  49. We conclude that FADS2 genotype may play a role in peroxidation susceptibility of lipoproteins PMID: 23721366
  50. These data suggested that FADS1 genetic variant was associated with circulating PUFA and lipid levels and that its effect on HDL cholesterol might depend on PUFA status in the Han Chinese population. PMID: 23396965
  51. Single Nucleotide Polymorphism in FADS1 gene is associated with type 2 diabetes. PMID: 22992776
  52. FADS snps can affect age-associated changes in serum PUFAs, Delta5-desaturase and oxidative stress in middle-aged nonobese men. The rs174537T allele did not show increases in AA and Delta5-desaturase activity seen with the rs174537GG genotype. PMID: 23818766
  53. These findings suggest that the polymorphisms of rs174556 in the FADS1 gene are very likely to be associated with acute coronary syndrome in Chinese Han population, especially in subjects with hypertension PMID: 23555103
  54. study concludes different sites at the FADS1-FADS2 locus appear to influence Delta-5 and Delta-6 desaturase (D5D and D6D) activity, and rs174537 genotype interacts with dietary eicosapentaenoic acid and docosahexaenoic acid to modulate D5D PMID: 23160180
  55. genetic association study in maternal/child dyads in England: Data suggest that SNPs in FADS1 (and in FADS2/FADS3) influence fetal fatty acid metabolism; both maternal and child FADS genotypes/haplotypes influence cord plasma long-chain fatty acids. PMID: 22877655
  56. Data suggest that mRNA expression of delta-5 and delta-6 desaturases are down-regulated 40-50% in vitamin B-6-restricted hepatocytes. PMID: 23211517
  57. the minor haplotype variant of the FADS1 gene was associated with an increase in dihomo-gamma linolenic acid (DGLA) level in human brain tissue. PMID: 22900039
  58. Findings suggest that the 3 genes, FADS1, FADS2, and DCHS2, containing these SNPs might play dual roles influencing both CSI and ALM in males. PMID: 22960237
  59. In minor allele homozygotes, increased alpha-linolenic acid intake resulted in increased plasma eicosapentaenoic acid composition. PMID: 23221573
  60. The aim of this study was to investigate the relation between these ratios as well as genotypes of FADS1 rs174547 and coronary heart disease incidence. PMID: 22911844
  61. rs174556 in FADS1 gene and rs174617 in FADS2 gene may not be associated with paranoid schizophrenia. PMID: 21560298
  62. A single nucleotide polymorphism and a haplotype in intron 1 of FADS2 were significantly associated with FADS1 expression. PMID: 22748975
  63. Total cholesterol, HDL, LDL and triglyceride concentrations may be influenced by the FADS1 FADS2 genotype already in 10 year old children. PMID: 22629455
  64. discovery and function of a novel FADS1 splice variant PMID: 22619218
  65. rs174547 in FADS1 may contribute to the susceptibility of CHD by altering HDL-C and TG levels in Chinese individuals. PMID: 22490578
  66. The association between dietary intake of fatty acids and allergic diseases might be modulated by FADS gene variants in children. PMID: 21793953
  67. Report relationship of serum fatty acid composition and desaturase activity to C-reactive protein in Japanese men and women. PMID: 22153152
  68. genetic association studies in African Americans and European Americans: Seven SNP in FADS gene cluster are associated with plasma omega-6/omega-3 fatty acids; data suggest ethnic differences in synthesis of long-chain polyunsaturated fatty acids. PMID: 21733300
  69. rs174556 in the FADS1 gene is very likely to be associated with coronary artery disease in the Chinese Han population PMID: 21917437
  70. Correlation coefficients were estimated to describe fatty acid tracking over 4 years and to assess the influence of FADS variants on tracking. PMID: 21818279
  71. impact of FADS genetic variants on PUFA metabolism, specifically AA levels, is likely more pronounced in African Americans due to larger proportion of individuals carrying the genotype associated with increased FADS1 enzymatic conversion of DGLA to AA PMID: 21599946
  72. These results confirm associations of age-related macular degeneration with variants near the TIMP3 gene and at loci involved in HDL metabolism PMID: 21613373
  73. This study shows that genetic variation in the FADS gene cluster (in particular rs174547) can alter desaturase activity in subjects of Caucasians and Asian descent. PMID: 21414826
  74. Not being breastfed conferred an 8- to 9-point disadvantage in cognition among children GG homozygote for rs174468 (low FADS1 activity) but not among those with the A allele. PMID: 21383846
  75. increased expression of fatty acid Delta5- and Delta6-desaturases in cystic fibrosis correlated with selective abnormalities in essential fatty acid concentrations PMID: 21605700
  76. assessed whether dietary linoleic acid or alpha-linolenic acid modulate the association between the FADS1 rs174546 polymorphism and concentrations of PUFA, other lipids, and lipoproteins in adolescents PMID: 21593353
  77. The rs174537T polymorphism of FADS1 is associated with a lower proportion of arachidonic acid in serum phospholipids and reduced coronary artery disease risk, in association with reduced total- and LDL-cholesterol and lipid peroxides in Koreans PMID: 21040914
  78. PUFA-composition in young children's blood is under strong control of the FADS-gene-cluster PMID: 20948998
  79. stearoyl coenzyme A desaturase (SCD) and D6D activity, were directly related to diabetes risk in multivariable-adjusted models. PMID: 20980488
  80. Genetic variants of the fatty acid desaturase gene cluster predict amounts of red blood cell docosahexaenoic and other polyunsaturated fatty acids in pregnant women. PMID: 21106917
  81. potential role in disease onset and development (Review) PMID: 20565855
  82. Genetic variation in the FADS1 FADS2 gene cluster affects n-6 polyunsaturated fatty acid profiles in erythrocytes reflecting altered delta-5-desaturase activity. PMID: 20691134
  83. association between genetic variability in the FADS gene cluster and delt-5 and delta-6 desaturase activities PMID: 20427696
  84. Liver Delta-6D and Delta-5D activities in obese patients were 87% and 66% lower than controls (P < 0.001) PMID: 19875987
  85. impact of FADS1 genotype on LC-PUFA/lipid metabolism and influence on intellectual development in infants and chronic metabolic diseases [REVIEW] PMID: 19948371
  86. Genetic variation in the FADS1 gene potentially interacts with dietary polyunsaurated faatty acid intakes which in turn affects plasma cholesterol concentrations. PMID: 20484448
  87. Strong associations between variants in the human genes FADS1, and blood levels of polyunsaturated fatty acids have been reported--REVIEW PMID: 20045144
  88. A single nucleotide polymorphism in the FADS1/FADS2 gene is associated with plasma lipid profiles in two genetically similar Asian ethnic groups with distinctive differences in life style. PMID: 20364269
  89. Immunofluorescence confocal microscopy performed on BHK-21 and Caco-2 cell lines transiently expressing the two human isoforms, definitively confirmed that hFADS1, but not hFADS2, localizes in mitochondria. PMID: 20060505
  90. Lower proportions of docosahexenoic acid in milk from women homozygous for a minor allele in t4his gene could not be compensated for by increasing fish and fish-oil intake. PMID: 20335541
  91. This is the first report on characterization of human FADS, and the first cloning and over-expression of FADS from an organism higher than yeast. PMID: 16643857
  92. FADS1 FADS2 genetic varients and their reconstructed haplotypes are associated with the fatty acid composition in phospholipids PMID: 16670158
  93. support a significant role for reverse Delta5-desaturase as a natural antisense regulator of Delta5-desaturase PMID: 16846730
  94. SREBP-1 is involved in the early regulation of delta5 desaturase gene by simvastatin, in THP-1 cells. PMID: 17655842
  95. The results indicate that when the supply of FA to HL60 cells is limited, the intracellular content of n-3 and n-6 FA decreases and this leads to upregulation of the desaturases, particularly D5D and D6D. PMID: 17852835
  96. strong association of FADS1 gene polymorphisms with the levels of arachidonic acid, which is a precursor of molecules involved in inflammation and immunity processes, cardiovascular disease. PMID: 18320251
  97. In populations following a Western diet, subjects carrying FADS haplotypes that are associated with higher desaturase activity may be prone to a proinflammatory response favoring atherosclerotic vascular damage. PMID: 18842780
  98. This study showed that genetic variants of FADS1 and FADS2 influence blood lipid and breast milk essential fatty acids in pregnancy and lactation. PMID: 18936223
  99. Single nucleotide polymorphisms (SNPs) in the 2 desaturase encoding gene FADS1 is highly associated with the concentration of omega-6 and omega-3 fatty acids. PMID: 19776639

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Subcellular Location Isoform 1: Endoplasmic reticulum membrane, Multi-pass membrane protein, Mitochondrion, SUBCELLULAR LOCATION: Isoform 2: Endoplasmic reticulum membrane, Multi-pass membrane protein
Protein Families Fatty acid desaturase type 1 family
Tissue Specificity Widely expressed, with highest levels in liver, brain, adrenal gland and heart. Highly expressed in fetal liver and brain.
Database Links

HGNC: 3574

OMIM: 606148

KEGG: hsa:3992

STRING: 9606.ENSP00000322229

UniGene: Hs.503546

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