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| Code | CSB-CF012086HU |
| Size | US$3587 |
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| Description |
This recombinant HumanKCNN4 protein is an in vitro E.coli (cell-free) expressed Full Length protein. Its purity is 85%+ determined by SDS-PAGE. Cell-free protein expression is the in vitro synthesis of a protein using translation-compatible extracts of whole cells. In principle, whole-cell extracts contain all the macromolecules and components needed for transcription, translation, and even post-translational modification. These components include RNA polymerase, regulatory protein factors, transcription factors, ribosomes, and tRNA. When supplemented with cofactors, nucleotides, and the specific gene template, these extracts can synthesize proteins of interest in a few hours. The KCNN4 is the encoding gene for the KCa3.1/SK4, which plays a critical role in calcium-activated anion secretion in mice and humans. It is abundantly expressed in non-excitable cells such as erythrocytes, lymphocytes, and placenta cells. The KCa3.1 has been found to participate in the pacemaker activity of cardiomyocytes sourcing from human embryonic stem cells (hESC-CMs). It is involved in cancer progression, including cell proliferation, apoptosis, epithelial-mesenchymal transition (EMT), and metastasis. |
| Purity | Greater than 85% as determined by SDS-PAGE. |
| Target Names | KCNN4 |
| Uniprot No. | O15554 |
| Research Area | Cell Biology |
| Alternative Names |
KCNN4; IK1; IKCA1; KCA4; SK4; Intermediate conductance calcium-activated potassium channel protein 4; SKCa 4; SKCa4; IKCa1; KCa3.1; KCa4; Putative Gardos channel
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| Species | Homo sapiens (Human) |
| Source | in vitro E.coli expression system |
| Expression Region | 1-427 aa |
| Target Protein Sequence | MGGDLVLGLGALRRRKRLLEQEKSLAGWALVLAGTGIGLMVLHAEMLWFGGCSWALYLFLVKCTISISTFLLLCLIVAFHAKEVQLFMTDNGLRDWRVALTGRQAAQIVLELVVCGLHPAPVRGPPCVQDLGAPLTSPQPWPGFLGQGEALLSLAMLLRLYLVPRAVLLRSGVLLNASYRSIGALNQVRFRHWFVAKLYMNTHPGRLLLGLTLGLWLTTAWVLSVAERQAVNATGHLSDTLWLIPITFLTIGYGDVVPGTMWGKIVCLCTGVMGVCCTALLVAVVARKLEFNKAEKHVHNFMMDIQYTKEMKESAARVLQEAWMFYKHTRRKESHAARRHQRKLLAAINAFRQVRLKHRKLREQVNSMVDISKMHMILYDLQQNLSSSHRALEKQIDTLAGKLDALTELLSTALGPRQLPEPSQQSK Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. |
| Mol. Weight | 53.7 kDa |
| Protein Length | Full Length |
| Tag Info |
N-terminal 10xHis-tagged |
| Form |
Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. |
| Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Troubleshooting and FAQs |
Protein FAQs |
| Storage Condition | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Lead Time | Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time. |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Datasheet & COA | Please contact us to get it. |
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| Function |
Forms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T-cells. Plays a role in the late stages of EGF-induced macropinocytosis.
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| Gene References into Functions |
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| Involvement in disease | Dehydrated hereditary stomatocytosis 2 (DHS2) |
| Subcellular Location | Cell membrane; Multi-pass membrane protein. |
| Protein Families | Potassium channel KCNN family, KCa3.1/KCNN4 subfamily |
| Tissue Specificity | Widely expressed in non-excitable tissues. |
| Database Links |
HGNC: 6293 OMIM: 602754 KEGG: hsa:3783 STRING: 9606.ENSP00000262888 UniGene: Hs.10082 |
