Recombinant Mouse Long-chain-fatty-acid--CoA ligase 1(Acsl1)

Code CSB-CF001191MO
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names Acsl1
Uniprot No. P41216
Alternative Names Acsl1; Acsl2; Facl2; Long-chain-fatty-acid--CoA ligase 1; Arachidonate--CoA ligase; Long-chain acyl-CoA synthetase 1; LACS 1; Phytanate--CoA ligase
Species Mus musculus (Mouse)
Expression Region 1-699
Target Protein Sequence MEVHELFRYFRMPELIDIRQYVRTLPTNTLMGFGAFAALTTFWYATRPKALKPPCDLSMQSVEIAGTTDGIRRSAVLEDDKLLVYYYDDVRTMYDGFQRGIQVSNNGPCLGSRKPNQPYEWISYKEVAELAECIGSGLIQKGFKPCSEQFIGLFSQNRPEWVIVEQGCFSYSMVVVPLYDTLGADAITYIVNKAELSVIFADKPEKAKLLLEGVENKLTPCLKIIVIMDSYGSDLVERGKKCGVEIISLKALEDLGRVNRVKPKPPEPEDLAIICFTSGTTGNPKGAMITHQNIINDCSGFIKATESAFIASTDDVLISFLPLAHMFETVVECVMLCHGAKIGFFQGDIRLLMDDLKVLQPTIFPVVPRLLNRMFDRIFGQANTSLKRWLLDFASKRKEAELRSGIVRNNSLWDKLIFHKIQSSLGGKVRLMITGAAPVSATVLTFLRTALGCQFYEGYGQTECTAGCCLSLPGDWTAGHVGAPMPCNYVKLVDVEEMNYLASKGEGEVCVKGANVFKGYLKDPARTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAPEKIENIYLRSEAVAQVFVHGESLQAFLIAVVVPDVESLPSWAQKRGLQGSFEELCRNKDINKAILDDLLKLGKEAGLKPFEQVKGIAVHPELFSIDNGLLTPTLKAKRPELRNYFRSQIDELYATIKI
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitoleate, oleate and linoleate. Preferentially activates arachidonate than epoxyeicosatrienoic acids (EETs) or hydroxyeicosatrienoic acids (HETEs).
Gene References into Functions
  1. ACSL1-mediated metabolic trapping of exogenous LCFA accelerates LCFA uptake rates, albeit to a lesser extent in females, which distinctly affects LCFA trafficking to acyl intermediates but not triglyceride storage or mitochondrial oxidation and is affected by female sex hormones. PMID: 26995156
  2. Data (including data from studies in cell line from knockout mice) suggest high incorporation of long-chain fatty acids, partly mediated by Acsl1, plays role in supply of octanoic acid for ghrelin acylation/lipoylation in ghrelin-producing cells. PMID: 26991015
  3. Acyl-CoA synthetase 1 deficiency alters cardiolipin species and impairs mitochondrial function PMID: 26136511
  4. ACSL1 is a programmable mediator of insulin sensitivity and cellular lipid content. PMID: 25915184
  5. long-chain acyl-CoA synthetase isoform 1 (ACSL1) deficiency in the heart activated mTORC1, thereby inhibiting autophagy and increasing the number of damaged mitochondria. PMID: 26220174
  6. Acsl1(M-/-) mice were more insulin sensitive, and, during an overnight fast, their respiratory exchange ratio was higher, indicating greater glucose use and during endurance exercise, Acsl1(M-/-) mice ran only 48% as far as controls. PMID: 25071025
  7. These data indicate that Acsl1-deficiency causes diastolic dysfunction and that mTOR activation is linked to the development of cardiac hypertrophy in Acsl1(H-/-) mice. PMID: 24631848
  8. Data indicate that the expression levels of ACSL1 and its metabolite triglyceride levels were remarkably increased in hepatitis B virus X protein (HBx)-induced liver cancer tissues from the HBx transgenic mice model. PMID: 24462768
  9. Increases understanding of the role of ACSL1 in monocytes/macrophages in inflammation and diabetes-accelerated atherosclerosis offers hope for new treatment to combat diabetic vascular disease[review] PMID: 23153419
  10. Acyl-CoA synthetase 1 is induced by Gram-negative bacteria and lipopolysaccharide and is required for phospholipid turnover in stimulated macrophages PMID: 23426369
  11. Endothelial ACSL1 is not required for inflammatory and apoptotic effects of a saturated fatty acid-rich environment. PMID: 23241406
  12. the reduced ABCA1 and cholesterol efflux in macrophages subjected to conditions of diabetes and elevated fatty load may, at least in part, be mediated by ACSL1 PMID: 22020260
  13. Acsl1 knockdown stimulated expression of lipogenic genes. PMID: 22445754
  14. ACSL1 plays a critical role by promoting the inflammatory phenotype of macrophages associated with type 1 diabetes PMID: 22308341
  15. Acsl1 is required for heart fatty acid oxidation. Heart-specific Acsl1 deficiency causes cardiac hypertrophy. PMID: 21245374
  16. Acsl1(A-/-) adipocytes incorporated [(14)C]oleate into glycerolipids normally, but fatty acid oxidation rates were 50%-90% lower than in control adipocytes and mitochondria. PMID: 20620995
  17. O-GlcNAc interrupts a known interaction between Sp1 and sterol regulatory element binding protein 2 (SREBP2), thereby inhibiting expression of the gene encoding acetyl-CoA synthetase 1, which is involved in lipid synthesis. PMID: 20138838
  18. Normoleptinemic control ACS-transgenic mice developed severe dilated cardiomyopathy with thickened left ventricular walls and profound impairment of systolic function on echocardiogram PMID: 15347805
  19. Together, our findings suggest that a constitutive interaction between FATP1 and ACSL1 contributes to the efficient cellular uptake of LCFAs in adipocytes through vectorial acylation. PMID: 16357361
  20. primary role of ACSL1 in adipocytes not in control of lipid influx, as previously considered, but in lipid efflux and fatty acid-induced insulin resistance PMID: 19429676
  21. Acs as a cancer survival factor: its inhibition enhances the efficacy of etoposide. PMID: 19459852
  22. Results describe a knock-out model of acyl-CoA synthetase-1 (ACSL1) and its effects in liver and in whole body metabolism. PMID: 19648649

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Subcellular Location Mitochondrion outer membrane; Single-pass type III membrane protein. Peroxisome membrane; Single-pass type III membrane protein. Microsome membrane; Single-pass type III membrane protein. Endoplasmic reticulum membrane; Single-pass type III membrane protein.
Protein Families ATP-dependent AMP-binding enzyme family
Database Links

KEGG: mmu:14081

STRING: 10090.ENSMUSP00000034046

UniGene: Mm.210323

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