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Product Name:

Human Homocysteine(HCY)ELISA Kit

Product Type : ELISA Kit
Code : CSB-E13814h
Size : 96T,5×96T,10×96T
Abbreviation : Hcy
Alias : N/A
Species : Human
Sample Types : serum, plasma, urine and tissue homogenates
Detect Range : 0.78 nmol/ml -50 nmol/ml
Sensitivity : 0.195 nmol/ml
Assay Time : 1-5h
Sample Volume : 50-100ul
Detection Wavelengt : 450 nm
Availability : 3-5 working days
Protocol :
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Precision :
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity :
To assess the linearity of the assay, samples were spiked with high concentrations of human HCY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %85
Range %80-90
1:2Average %98
Range %91-105
1:4Average %89
Range %84-96
1:8Average %93
Range %86-98
Recovery :
The recovery of human HCY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9691-101
EDTA plasma (n=4)9289-94
Typical Data :
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
nmol/mlOD1OD2AverageCorrected
502.082 2.156 2.119 1.997
251.456 1.489 1.473 1.351
12.50.941 0.968 0.955 0.833
6.250.583 0.597 0.590 0.468
3.1250.342 0.367 0.355 0.233
1.560.248 0.261 0.255 0.133
0.780.192 0.198 0.195 0.073
00.121 0.123 0.122
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