Mouse Homocysteic acid,Hcy ELISA Kit

Mouse Homocysteic acid,Hcy ELISA Kit

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Mouse Homocysteic acid,Hcy ELISA Kit

Citations (1) Protocol

Code	CSB-E08897m
Size	96T,5×96T,10×96T
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Product Details
Citations (1)
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Description

This Mouse Hcy ELISA Kit was designed for the quantitative measurement of Mouse Hcy protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 15.6 pmol/mL-1000 pmol/mL and the sensitivity is 3.9 pmol/mL.

Target Name

Homocysteic acid,Hcy

Alternative Names

N/A

Abbreviation

Hcy

Species

Mus musculus (Mouse)

Sample Types

serum, plasma

Detection Range

15.6 pmol/mL-1000 pmol/mL

Sensitivity

3.9 pmol/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Hcy in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	98
	Range %	93-102
1:40	Average %	100
	Range %	95-105
1:80	Average %	97
	Range %	91-103
1:160	Average %	93
	Range %	88-99

Recovery

The recovery of mouse Hcy spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	82-95
EDTA plasma (n=4)	99	96-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pmol/ml	OD1	OD2	Average	Corrected
1000	2.749	2.800	2.775	2.578
500	2.032	2.113	2.073	1.876
250	1.365	1.386	1.376	1.179
125	0.848	0.904	0.876	0.679
62.5	0.543	0.563	0.553	0.356
31.2	0.372	0.403	0.388	0.191
15.6	0.278	0.302	0.290	0.093
0	0.198	0.195	0.197

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Folic acid prevents cardiac dysfunction and reduces myocardial fibrosis in a mouse model of high-fat diet-induced obesity Li W.et al,Nutr Metab (Lond),2017

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The Overview of Organelles ELISA Data Analysis How to Do The Pre-experiment before Your ELISA Experiment Four Types of ELISA 11 tips for choosing your right ELISA kit Do you Often Trouble in These Problems of ELISA? Problems and Solutions in ELISA Experiments

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.