Mouse Homocysteic acid,Hcy ELISA Kit

Code CSB-E08897m
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now

Product Details

Target Name
Homocysteic acid,Hcy
Alternative Names
N/A
Abbreviation
Hcy
Species
Mus musculus (Mouse)
Sample Types
serum, plasma
Detection Range
15.6 pmol/mL-1000 pmol/mL
Sensitivity
3.9 pmol/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse Hcy in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:20Average %98
Range %93-102
1:40Average %100
Range %95-105
1:80Average %97
Range %91-103
1:160Average %93
Range %88-99
Recovery
The recovery of mouse Hcy spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9082-95
EDTA plasma (n=4)9996-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pmol/mlOD1OD2AverageCorrected
10002.749 2.800 2.775 2.578
5002.032 2.113 2.073 1.876
2501.365 1.386 1.376 1.179
1250.848 0.904 0.876 0.679
62.50.543 0.563 0.553 0.356
31.20.372 0.403 0.388 0.191
15.60.278 0.302 0.290 0.093
00.198 0.195 0.197  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Mouse Hcy ELISA Kit was designed for the quantitative measurement of Mouse Hcy protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 15.6 pmol/mL-1000 pmol/mL and the sensitivity is 3.9 pmol/mL.

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