Mouse Homocysteic acid,Hcy ELISA Kit

Code CSB-E08897m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


This Mouse Hcy ELISA Kit was designed for the quantitative measurement of Mouse Hcy protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 15.6 pmol/mL-1000 pmol/mL and the sensitivity is 3.9 pmol/mL.

Target Name Homocysteic acid,Hcy
Alternative Names
Abbreviation Hcy
Species Mus musculus (Mouse)
Sample Types serum, plasma
Detection Range 15.6 pmol/mL-1000 pmol/mL
Sensitivity 3.9 pmol/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of mouse Hcy in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:20Average %98
Range %93-102
1:40Average %100
Range %95-105
1:80Average %97
Range %91-103
1:160Average %93
Range %88-99
The recovery of mouse Hcy spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9082-95
EDTA plasma (n=4)9996-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
10002.749 2.800 2.775 2.578
5002.032 2.113 2.073 1.876
2501.365 1.386 1.376 1.179
1250.848 0.904 0.876 0.679
62.50.543 0.563 0.553 0.356
31.20.372 0.403 0.388 0.191
15.60.278 0.302 0.290 0.093
00.198 0.195 0.197  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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