Mouse Homocysteine(HCY)ELISA Kit

Mouse Homocysteine(HCY)ELISA Kit

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Customer Reviews and Q&A

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Mouse Homocysteine(HCY)ELISA Kit

Name: Mouse Homocysteine(HCY)ELISA Kit
Brand: CUSABIO
SKU: CSB-E16551m

Citations (3) Protocol

Code	CSB-E16551m
Size	96T,5×96T,10×96T
Trial Size
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Product Details
Citations (3)
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Customer Reviews and Q&A

Description

This Mouse Hcy ELISA Kit was designed for the quantitative measurement of Mouse Hcy protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 4.7 nmol/mL-300 nmol/mL and the sensitivity is 1.1 nmol/mL.

Target Name

Homocysteine(HCY)

Alternative Names

N/A

Abbreviation

Hcy

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

4.7 nmol/mL-300 nmol/mL

Sensitivity

1.1 nmol/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Hcy in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-105
1:2	Average %	90
	Range %	86-96
1:4	Average %	94
	Range %	90-98
1:8	Average %	99
	Range %	95-107

Recovery

The recovery of mouse Hcy spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	80-88
EDTA plasma (n=4)	95	91-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

nmol/ml	OD1	OD2	Average	Corrected
300	2.437	2.352	2.395	2.222
150	2.075	2.036	2.056	1.883
75	1.674	1.604	1.639	1.466
37.5	1.079	1.132	1.106	0.933
18.75	0.621	0.597	0.609	0.436
9.4	0.416	0.459	0.438	0.265
4.7	0.297	0.289	0.293	0.120
0	0.179	0.167	0.173

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Epigallocatechin-3-gallate Enhances Cognitive and Memory Performance and Protects Against Brain Injury in Methionine-induced Hyperhomocysteinemia Through Interdependent Molecular Pathways MD Mostafa,Neurotoxicity research,2022
Nanoformulated ellagic acid ameliorates pentylenetetrazol-induced experimental epileptic seizures by modulating oxidative stress, inflammatory cytokines and apoptosis in the brains of male mice El-Missiry M A, et al,Metabolic Brain Disease,2019
Role of osteocytes in mediating bone mineralization during hyperhomocysteinemia Vijayan V.et al,J Endocrinol,2017

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■ Q&A

I want to know some information regarding the kit CSB-E16551M:
The kit is labeled as 4.7-300 nmol/ml, which I assume is nmole/ml. Units are notmally expressed in nM, that is nmoles per liter (nmole/L). Could you confirm the unit is correct?

Thanks for your inquiry.
This kit CSB-E16551m is specific to detect Homocysteine. Usually nM is nmol/L，but most literatures use nmol/mL(some literatures use nM, and it is due to habit) .
If you want to use nM, pls convert the unit. Pls let me know if you have any further questions. Thank you.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.