Rat Homocysteic acid,Hcy ELISA Kit

Code CSB-E08896r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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Product Details

Target Name
Homocysteic acid,Hcy
Alternative Names
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.78 nmol/mL-50 nmol/mL
0.195 nmol/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rat Hcy in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %104
Range %100-108
1:2Average %96
Range %92-100
1:4Average %94
Range %90-98
1:8Average %89
Range %84-93
The recovery of rat Hcy spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8985-93
EDTA plasma (n=4)9794-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
nmol/ml. OD1OD2AverageCorrected
502.331 2.368 2.350 2.218
251.626 1.637 1.632 1.500
12.51.089 1.074 1.082 0.950
6.250.679 0.678 0.679 0.547
3.120.467 0.445 0.456 0.324
1.560.341 0.368 0.355 0.223
0.780.285 0.268 0.277 0.145
00.131 0.133 0.132  
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Rat Hcy ELISA Kit was designed for the quantitative measurement of Rat Hcy protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 nmol/mL-50 nmol/mL and the sensitivity is 0.195 nmol/mL .

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