Human Odontogenic ameloblast-associated protein(ODAM) ELISA kit

Code CSB-EL016268HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
odontogenic, ameloblast asssociated
Alternative Names
ODAM ELISA Kit; APIN ELISA Kit; Odontogenic ameloblast-associated protein ELISA Kit; Apin ELISA Kit
Abbreviation
ODAM
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.156 ng/mL-10 ng/mL
Sensitivity
0.04 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ODAM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:5Average %104
Range %99-109
1:10Average %98
Range %95-102
1:20Average %84
Range %80-88
1:40Average %90
Range %85-94
Recovery
The recovery of human ODAM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9690-102
EDTA plasma (n=4)9389-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.682 2.790 2.736 2.564
52.079 2.005 2.042 1.870
2.51.310 1.254 1.282 1.110
1.250.773 0.729 0.751 0.579
0.6250.458 0.423 0.441 0.269
0.3120.374 0.367 0.371 0.199
0.1560.257 0.269 0.263 0.091
00.171 0.173 0.172
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human ODAM ELISA Kit was designed for the quantitative measurement of Human ODAM protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.04 ng/mL.

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Target Background

Function
(From Uniprot)
Tooth-associated epithelia protein that probably plays a role in odontogenesis, the complex process that results in the initiation and generation of the tooth. May be incorporated in the enamel matrix at the end of mineralization process. Involved in the induction of RHOA activity via interaction with ARHGEF and expression of downstream factors such as ROCK. Plays a role in attachment of the junctional epithelium to the tooth surface.
Gene References into Functions
  1. Risk of recurrence correlated with cytoplasmic expression of ODAM and pAKT, whereas nuclear expression did not predict recurrence of gastrointestinal stromal tumors. PMID: 27229116
  2. The inhibitory effects of ODAM on colorectal cancer cell growth were associated with PTEN elevation and PI3K/AKT signaling inactivation. PMID: 27694004
  3. ODAM induced RhoA activity. ODAM-mediated RhoA signalling resulted in actin filament rearrangement, increased cell adhesion and inhibited the migration/invasion of MCF7 cells. These results suggest that ODAM expression maintains their adhesion, resulting in the prevention of their metastasis via the regulation of RhoA signalling in breast cancer cells. PMID: 26358398
  4. We propose that ODAM has diverse functions that vary with protein location in various cell lines. Nuclear ODAM appears to be associated with MMP-20 regulation and tumorigenesis. Additional functions may take place in cytoplasm and extracellular matrix. PMID: 22387195
  5. Our studies demonstrate that ectopic ODAM expression in melanoma cell lines suppresses growth and migratory activity in these cells, while eliciting elevated PTEN expression and suppression of AKT activity. PMID: 23648148
  6. The expression of APIN at an early time-point following disruption of periodontal integrity suggests that this protein may be part of the cascade of events leading to the activation of ERM during periodontal healing and regeneration PMID: 20572917
  7. Calcifying epithelial odontogenic tumor-associated amyloid consists of a unique and previously undescribed protein that we provisionally designate APin. PMID: 14647039
  8. The protein sequences deduced from the cloned cDNA for rat, mouse, pig, and human were aligned together with those obtained from four other mammal genomes. Apin is highly conserved in mammals. PMID: 17647262
  9. Data suggest that ODAM is a developmental antigen that has an essential role in tooth maturation and in the pathogenesis of certain odontogenic and other epithelial neoplasms PMID: 18472969
  10. Confirmation of the amyloidogenic potential of ODAM has resulted from analyses of four other cases where we found, in addition, a 74-residue segment specified by exon 4. PMID: 18484335

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Subcellular Location
Secreted. Cytoplasm. Nucleus.
Protein Families
ODAM family
Tissue Specificity
Expressed in the junctional epithelium of healthy teeth. In periodontitis, absent in the pocket epithelium of the diseased periodontium but is detected in the gingival crevicular fluid.
Database Links

HGNC: 26043

OMIM: 614843

KEGG: hsa:54959

STRING: 9606.ENSP00000379401

UniGene: Hs.143811

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