Target Name
angiopoietin 1
Alternative Names
ANGPT1Angiopoietin-1 ELISA kit; ANG-1 ELISA kit
Sample Types
serum, plasma, tissue homogenates
Detection Range
6 ng/mL-200 ng/mL
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Precision
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of pig ANG-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
90-105 |
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| 1:2 |
Average % |
95 |
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| Range % |
85-100 |
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| 1:4 |
Average % |
89 |
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| Range % |
85-101 |
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| 1:8 |
Average % |
90 |
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| Range % |
80-99 |
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Recovery
| The recovery of pig ANG-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
92-105 |
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| EDTA plasma (n=4) |
96 |
90-102 |
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Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
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| 200 |
0.345 |
0.351 |
0.348 |
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| 100 |
0.478 |
0.486 |
0.482 |
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| 50 |
0.703 |
0.716 |
0.710 |
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| 17 |
1.068 |
1.103 |
1.086 |
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| 6 |
1.316 |
1.355 |
1.336 |
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| 0 |
1.925 |
1.968 |
1.947 |
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Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
