Product Details

Purity

>97% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human lymphocytes is in a concentration range of 1.0-10.0 ng/ml.

Target Names

CCL28

Uniprot No.

Q9NRJ3

Research Area

Immunology

Alternative Names

C C motif chemokine ligand 28; C-C motif chemokine 28; CC chemokine CCL28; CCK 1; CCK1; CCK1 protein; CCL 28; CCL28; CCL28_HUMAN; Chemokine (C-C motif) ligand 28; chemokine (C-C motif) ligand 28 splice variant chi; MEC; Mucosae associated epithelial chemokine; Mucosae-associated epithelial chemokine; Protein CCK1; SCYA28; Small inducible cytokine A28 [Precursor]; small inducible cytokine subfamily A (Cys-Cys); member 28; Small-inducible cytokine A28

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

20-127aa

Complete Sequence

SEAILPIASS CCTEVSHHIS RRLLERVNMC RIQRADGDCD LAAVILHVKR RRICVSPHNH TVKQWMKVQA AKKNGKGNVC HRKKHHGKRN SNRAHQGKHE TYGHKTPY

Mol. Weight

12.4 kDa

Protein Length

Full Length of Mature Protein

Tag Info

Tag-Free

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 µm filtered PBS, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

CCL28, also known as mucosae-associated epithelial chemokine, plays a critical role in directing lymphocyte homing to mucosal tissues, making it a key target in mucosal immunity and inflammatory disease research. This tag-free recombinant human CCL28 encompasses the full-length mature protein (residues 20–127) and demonstrates robust chemotactic activity on human lymphocytes at 1.0–10.0 ng/ml, providing a suitable basis for Transwell migration assays, immune cell recruitment studies, and in vivo models of mucosal inflammation or infection. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that can confound chemotaxis readouts and downstream signaling analyses involving pathways such as MAPK and NF-κB. Purity exceeding 97% by SDS-PAGE, combined with this stringent endotoxin control, supports use as a reference standard in chemokine detection assays and as a reliable coating antigen or positive control for antibody development and ELISA validation.

Target Background

Function

Chemotactic activity for resting CD4, CD8 T-cells and eosinophils. Binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner.

Gene References into Functions

this review discusses the role of CCL28 in innate and adaptive immunity PMID: 28843907
Results show that CCL28 expression was up-regulated under hypoxic condition in lung adenocarcinoma cells. Besides other effects on tumor biology such as immunosuppression, CCL28 could promote angiogenesis in lung adenocarcinoma by directly activating its receptor, CCR3, on microvascular endothelial cells. PMID: 27250766
our results show for the first time that CCL28 contributes to breast cancer progression through the ERK/MAPKmediated anti-apoptotic and metastatic signaling pathway. Antagonists of CCL28 and the MAPK signaling pathway may be used synergistically to treat breast cancer patients. PMID: 28713975
HCC recruits Tregs to promote angiogenesis under hypoxic condition by upregulating CCL28 expression. These findings establish a link between Tregs and hypoxia in HCC growth and may provide a new potential therapeutic target for treating HCC. PMID: 27716621
CCL28 was absent in saliva of primary Sjogren's syndrome patients. This finding did not correlate with salivary IgA levels. PMID: 25567740
disease severity of atopic dermatitis in children is not correlated to the level of CCL28, but rather related to that of total IgE PMID: 26642722
CCL28-CCR3 interactions are involved in the homeostatic trafficking of CD4(+) T cells to the upper airways. PMID: 24917456
High CCL28 gene methylation is associated with gastric tumor aggressiveness. PMID: 25740824
CCL28 is a potent growth-promoting factor with the ability to support the in vitro and in vivo functional properties of cultured human hematopoietic cells. PMID: 23509159
These results indicate a role for IL-17A in the human lung by enhancing the expression of CCL28 and hence driving the recruitment of IgE-secreting B cells. PMID: 21447959
tumour hypoxia promotes the recruitment of regulatory T (T(reg)) cells through induction of expression of the chemokine CC-chemokine ligand 28 (CCL28), which, in turn, promotes tumour tolerance and angiogenesis PMID: 21753853
CCL28 may play dual roles in mucosal immunity as a chemoattractant for cells expressing CCR10 and/or CCR3 such as plasma cells and also as a broad-spectrum antimicrobial protein secreted into low-salt body fluids. PMID: 12538707
CCR10 and its mucosal epithelial ligand CCL28 have roles in the migration of circulating IgA plasmablasts PMID: 12671049
CCL28 produced by keratinocytes is mediated by different signal pathways from CCL27, and both CCL27 and CCL28 are involved in the pathogenesis of inflammatory skin diseases. PMID: 16433680
CCL28 is one link between microbial insult and the exacerbation of pathologies such as asthma, through an NFkappaB-dependent mechanism PMID: 16581045
Expression of CCL28 by epithelial cells from chronically inflamed liver in response to microbial products or interleukin-1 provides a signal to localize CCR10-expressing regulatory T cells at mucosal surfaces. PMID: 16785557
CCL28 mediates mucosal immunity in HIV exposure and infection PMID: 17912348
These studies are the first to show increased CCL28 production during gastrointestinal infection in humans and provide an explanation for the large influx of IgA-secreting cells to the gastric mucosa in H. pylori-infected individuals. PMID: 18426876
There was a robust migration of specific IgA- and IgM-antibody-secreting cells induced by Salmonella vaccination toward the mucosal chemokines CCL25 and CCL28 PMID: 19003934
data reinforce concept that CCL28 may contribute to the pathogenesis of atopic dermatitis probably through selective migration & infiltration of effector/memory T-helper-2 cells in the skin; CCL28 may represent a prognostic marker for disease severity PMID: 19659860

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Subcellular Location

Secreted.

Protein Families

Intercrine beta (chemokine CC) family

Tissue Specificity

Preferentially expressed by epithelial cells of diverse tissues including normal and pathological colon, salivary gland, mammary gland, trachea and rectum. Also found in prostate, spleen, thyroid, psoriasis skin and in lower levels in peripheral blood leu

Database Links

HGNC: 17700

OMIM: 605240

KEGG: hsa:56477

STRING: 9606.ENSP00000354416

UniGene: Hs.656904

Code	CSB-AP001011HU
Abbreviation	Recombinant Human CCL28 protein (Active)
MSDS	MSDS Datasheet
Size	$142
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Recombinant Human C-C motif chemokine 28 protein (CCL28) (Active)

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Target Background

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