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Recombinant Mouse C-C motif chemokine 28 protein (Ccl28), partial (Active)

Recombinant Mouse C-C motif chemokine 28 protein (Ccl28), partial (Active)

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Code	CSB-AP001081MO
Abbreviation	Recombinant Mouse Ccl28 protein, partial (Active)
MSDS	MSDS Datasheet
Size	$142
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Product Details
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Target Background

Product Details

Purity

>97% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using murine lymphocytes is in a concentration range of 1.0-10.0 ng/ml.

Target Names

Ccl28

Uniprot No.

Q9JIL2

Research Area

Immunology

Alternative Names

Ccl28; Scya28; C-C motif chemokine 28; Small-inducible cytokine A28

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

20-130aa

Complete Sequence

SEAILPMASS CCTEVSHHVS GRLLERVSSC SIQRADGDCD LAAVILHVKR RRICISPHNR TLKQWMRASE VKKNGRENVC SGKKQPSRKD RKGHTTRKHR TRGTHRHEAS R

Mol. Weight

12.6 kDa

Protein Length

Partial

Tag Info

Tag-Free

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 µm filtered PBS, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

CCL28 plays a critical role in mucosal immunity by directing lymphocyte homing to epithelial tissues, making it a key target for studying gut- and lung-associated immune responses. This tag-free recombinant mouse CCL28 (residues 20–130) encompasses the mature protein sequence and demonstrates potent chemotactic activity toward murine lymphocytes at 1.0–10.0 ng/ml, providing a suitable basis for Transwell migration assays, mucosal inflammation models, and signaling pathway studies involving CCR3/CCR10 receptor activation. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts in sensitive immune cell assays, which satisfies the purity and endotoxin criteria typical for in vivo studies of mucosal trafficking and ex vivo lymphocyte functional assays. Produced in *E. coli* with greater than 97% purity by SDS-PAGE, this protein also supports use as a reference standard in chemokine detection assays or as a coating antigen for antibody validation by ELISA.

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Target Background

Function

Chemotactic for resting CD4, CD8 T-cells and eosinophils. Binds to CCR10 and induces calcium mobilization in a dose-dependent manner.

Gene References into Functions

Blockade of CCL28 or CCR10 during an acute severe respiratory paramyxoviral infection is sufficient to prevent the development of post-viral airway disease. However, the mechanism of action is unclear and requires further exploration. PMID: 28474501
These data suggest that CCL28 could be useful in enhancing the IgA immune response that will likely play a pivotal role in prophylactic HIV vaccines. PMID: 22066023
tumour hypoxia promotes the recruitment of regulatory T (T(reg)) cells through induction of expression of the chemokine CC-chemokine ligand 28 (CCL28), which, in turn, promotes tumour tolerance and angiogenesis PMID: 21753853
demonstrate that interactions between the antimicrobial C-terminus of CCL28 with an appropriate CC chemokine N-terminal domain is important for the full antimicrobial activity of CCL28. PMID: 19830739
CCL28 has dual functions in mucosal immunity as a chemokine attracting cells expressing CCR10 and/or CCR3 and also as an apically secreted molecule with a potent antimicrobial activity against a broad spectrum of microbes. PMID: 12538707
MEC attracts IgA- but not IgG- or IgM-producing plasmablasts from both intestinal and nonintestinal lymphoid and effector tissues, including the intestines, lungs, and lymph nodes draining the bronchopulmonary tree and oral cavity. PMID: 12646646
Epithelial cells and venular endothelium of both small intestine and colon are positive for CCL28; CCL28 plays a direct role in intestinal homing of IgA antibody-secreting cells by mediating their extravasation into intestinal lamina propria. PMID: 15356112
CCL28 is a key regulator of IgA ASC accumulation in the mammary gland and thus controls the passive transfer of IgA antibodies from mother to infant. PMID: 15381732
CCL28 appears to play a role in regulating eosinophil recruitment to peribronchial regions of the lung possibly by coordinated temporal production with eotaxin PMID: 15681819
CCL28 mediates mucosal immunity in HIV exposure and infection PMID: 17912348

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Subcellular Location

Secreted.

Protein Families

Intercrine beta (chemokine CC) family

Tissue Specificity

Mainly expressed in testis, epithelial cells of normal colon, kidney, Peyer patches, lymph nodes. Also found in lower levels in brain, spleen and lung.

Database Links

KEGG: mmu:56838

STRING: 10090.ENSMUSP00000096847

UniGene: Mm.143745

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