Pde9a Antibody

Code CSB-PA530347ZA01MO
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Product Details

Full Product Name
Rabbit anti-Mus musculus (Mouse) Pde9a Polyclonal antibody
Uniprot No.
Target Names
Pde9a
Alternative Names
High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A (EC 3.1.4.35), Pde9a, Pde8b
Raised in
Rabbit
Species Reactivity
Mus musculus
Immunogen
Recombinant Mus musculus Pde9a protein
Immunogen Species
Mus musculus (Mouse)
Conjugate
Non-conjugate
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (12-14 weeks)

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Target Background

Function
Specifically hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes. Highly specific: compared to other members of the cyclic nucleotide phosphodiesterase family, has the highest affinity and selectivity for cGMP. Specifically regulates natriuretic-peptide-dependent cGMP signaling in heart, acting as a regulator of cardiac hypertrophy in myocytes and muscle. Does not regulate nitric oxide-dependent cGMP in heart. Additional experiments are required to confirm whether its ability to hydrolyze natriuretic-peptide-dependent cGMP is specific to heart or is a general feature of the protein (Probable). In brain, involved in cognitive function, such as learning and long-term memory.
Gene References into Functions
  1. Brain PDE9 is localized to preferentially regulate nuclear- and membrane-proximal pools of cGMP, and its function likely changes across the life span. PMID: 29505961
  2. PDE9A can regulate cGMP signalling independent of the nitric oxide pathway, and its role in stress-induced heart disease suggests potential as a therapeutic target. PMID: 25799991
  3. Data suggest that increases in hippocampal and amygdalar cGMP levels via blockade of PDE9 have prominent roles in consolidation of extinction learning (here, prevention of learning disorder due to cocaine neurotoxicity). PMID: 22596207
  4. This paper describes the human Pde9 gene but discusses the comparisons between the mouse and human Pde9 genes. PMID: 9624146
Subcellular Location
Cell projection, ruffle membrane. Cytoplasm, perinuclear region. Golgi apparatus. Endoplasmic reticulum. Cell membrane, sarcolemma.
Protein Families
Cyclic nucleotide phosphodiesterase family, PDE9 subfamily
Tissue Specificity
Highly expressed in kidney. Lower levels in liver, lung and brain. Widely expressed in brain, with highest expression in cerebellar Purkinje cells. Present in heart (at protein level).
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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