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Recombinant Mouse High affinity cGMP-specific 3,5-cyclic phosphodiesterase 9A (Pde9a)

Recombinant Mouse High affinity cGMP-specific 3,5-cyclic phosphodiesterase 9A (Pde9a)

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Code	CSB-EP530347MO
Abbreviation	Recombinant Mouse Pde9a protein
MSDS	MSDS Datasheet
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Target Background

Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

Pde9a

Uniprot No.

O70628

Research Area

Signal Transduction

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

1-534aa

Target Protein Sequence

MGAGSSSYRPKAIYLDIDGRIQKVVFSKYCNSSDIMDLFCIATGLPRNTTISLLTTDDAMVSIDPTMPANSERTPYKVRPVAVKQVSEREELIQGVLAQVAEQFSRAFKINELKAEVANHLAVLEKRVELEGLKVVEIEKCKSDIKKMREELAARNSRTNCPCKYSFLDNKKLTPRRDVPTYPKYLLSPETIEALRKPTFDVWLWEPNEMLSCLEHMYHDLGLVRDFSINPITLRRWLLCVHDNYRNNPFHNFRHCFCVTQMMYSMVWLCGLQEKFSQMDILVLMTAAICHDLDHPGYNNTYQINARTELAVRYNDISPLENHHCAIAFQILARPECNIFASVPPEGFRQIRQGMITLILATDMARHAEIMDSFKEKMENFDYSNEEHLTLLKMILIKCCDISNEVRPMEVAEPWVDCLLEEYFMQSDREKSEGLPVAPFMDRDKVTKATAQIGFIKFVLIPMFETVTKLFPVVEETMLRPLWESREHYEELKQLDDAMKELQKKTESLTSGAPENTTEKNRDAKDSEGHSPPN
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

65.7 kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Enhance your signal transduction studies with our Recombinant Mouse Pde9a, also known as High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A. Produced in E.coli, this protein plays a crucial role in deciphering the intricate pathways of cellular signaling.

Provided as a full-length protein (1-534aa), our Pde9a ensures a complete understanding of its function and structure. The protein is expressed with a N-terminal 6xHis-tag, providing streamlined purification and reliable stability. Our Recombinant Mouse Pde9a, with a purity of over 85% as determined by SDS-PAGE, meets the rigorous demands of scientific research. Choose between the liquid form for immediate use or lyophilized powder for long-term storage, to suit your research workflow.

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Target Background

Function

Specifically hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes. Highly specific: compared to other members of the cyclic nucleotide phosphodiesterase family, has the highest affinity and selectivity for cGMP. Specifically regulates natriuretic-peptide-dependent cGMP signaling in heart, acting as a regulator of cardiac hypertrophy in myocytes and muscle. Does not regulate nitric oxide-dependent cGMP in heart. Additional experiments are required to confirm whether its ability to hydrolyze natriuretic-peptide-dependent cGMP is specific to heart or is a general feature of the protein (Probable). In brain, involved in cognitive function, such as learning and long-term memory.

Gene References into Functions

Brain PDE9 is localized to preferentially regulate nuclear- and membrane-proximal pools of cGMP, and its function likely changes across the life span. PMID: 29505961
PDE9A can regulate cGMP signalling independent of the nitric oxide pathway, and its role in stress-induced heart disease suggests potential as a therapeutic target. PMID: 25799991
Data suggest that increases in hippocampal and amygdalar cGMP levels via blockade of PDE9 have prominent roles in consolidation of extinction learning (here, prevention of learning disorder due to cocaine neurotoxicity). PMID: 22596207
This paper describes the human Pde9 gene but discusses the comparisons between the mouse and human Pde9 genes. PMID: 9624146

Subcellular Location

Cell projection, ruffle membrane. Cytoplasm, perinuclear region. Golgi apparatus. Endoplasmic reticulum. Cell membrane, sarcolemma.

Protein Families

Cyclic nucleotide phosphodiesterase family, PDE9 subfamily

Tissue Specificity

Highly expressed in kidney. Lower levels in liver, lung and brain. Widely expressed in brain, with highest expression in cerebellar Purkinje cells. Present in heart (at protein level).

Database Links

KEGG: mmu:18585

STRING: 10090.ENSMUSP00000038005

UniGene: Mm.10812

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