Chicken Heat Shock Protein 70,Hsp-70 ELISA Kit

Code CSB-E11196Ch
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Description

This Chicken HSP70 ELISA Kit was designed for the quantitative measurement of Chicken HSP70 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.25 ng/mL-50 ng/mL and the sensitivity is 0.125 ng/mL.

Target Name Heat Shock Protein 70,Hsp-70
Alternative Names Heat shock 70 kDa protein ELISA Kit; HSP70 ELISA Kit
Abbreviation HSP70
Uniprot No. P08106
Species Gallus gallus (Chicken)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.25 ng/mL-50 ng/mL
Sensitivity 0.125 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of chicken Hsp-70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 83-97  
1:2 Average % 91  
Range % 85-98  
1:4 Average % 95  
Range % 91-99  
1:8 Average % 93  
Range % 84-99  
Recovery
The recovery of chicken Hsp-70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 98 92-104  
EDTA plasma (n=4) 96 90-102  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
0 2.576 2.518 2.547    
0.25 1.415 1.388 1.402    
1 1.035 1.047 1.041    
4 0.610 0.598 0.604    
12.5 0.329 0.314 0.322    
50 0.173 0.175 0.174    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)
Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release.
Gene References into Functions
  1. data indicate that HSP70 is part of the receptor complex of Avian infectious bronchitis virus (IBV) and might help to understand the mechanism of S-mediated cell entry of IBV PMID: 28224252
  2. variation in chicken HSP70 was abundant between and within species PMID: 25867297
  3. The effects of transport time on HSP70 expression, stress, and meat quality are reported. PMID: 25403192
  4. Mn had a dosage-dependent effect on HSP27, HSP40, HSP60, HSP70, and HSP90 mRNA expression in chicken spleen lymphocytes in vitro. PMID: 24081778
  5. Results of this study indicated a long-term enhancement of Hsp70 gene expression associated with improved thermotolerance acquisition in treated chicks without adversely affecting performance. PMID: 23787299
  6. It was concluded that dietary supplementation of Z. officinale and Z. zerumbet powder may induce HSP70 reaction in broiler chickens exposed to heat stress. PMID: 22533311
  7. Data suggest that the expression of HSP70 is heat inducible and tissue dependent and that heat induction may correlate with DNA methylation pattern in the HSP70 promoter. PMID: 23546965
  8. The effects of human interaction on HSP70 expression and stress hormone blood leves in female broiler chickens are reported. PMID: 23243228
  9. It appears that neonatal feed restriction can enhance resistance to Salmonella enteritidis colonization in heat-stressed broiler chicks, and the underlying mechanism could be associated with the lower expression of Hsp70. PMID: 22252346
  10. HSP 70 level increased both in vivo and in vitro trials and may be used as potential molecular and toxicological biomarker. PMID: 19826909
  11. Spinal cord overexpression of Hsp70 enhances motor neuron survival in vivo during the period of naturally occurring cell death. PMID: 16237177
  12. A study evaluating the relationship between tissue or allele and the expression of chicken hsp70 under normal growth conditions and during acute heat stress is reported. PMID: 16905471
  13. The interaction of Meq and Hsp70 is significant in Marek's disease virus oncogenesis. PMID: 19494050
  14. The results suggest that the improved tolerance to transport stress in chicks could be associated with better HSP 70 expression. PMID: 19531704

Show More

Hide All

Protein Families Heat shock protein 70 family
Database Links

KEGG: gga:423504

STRING: 9031.ENSGALP00000019120

UniGene: Gga.4942

Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
Address
7505 Fannin St. Ste 610-312, Houston, TX 77054, USA
Join Us with

Subscribe newsletter

Leave a message

© 2007-2022 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1