Human Adropin(ENHO) ELISA kit

Instructions
Code CSB-EL007669HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name energy homeostasis associated
Alternative Names Adropin ELISA Kit; C9orf165 ELISA Kit; Energy homeostasis associated protein ELISA Kit; Energy homeostasis-associated protein ELISA Kit; ENHO ELISA Kit; ENHO_HUMAN ELISA Kit; PRO830 ELISA Kit; UNQ470 ELISA Kit
Abbreviation ENHO
Uniprot No. Q6UWT2
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 pg/mL-100 pg/mL
Sensitivity 0.39 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ENHO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 94  
Range % 88-99  
1:200 Average % 90  
Range % 84-95  
1:400 Average % 98  
Range % 91-99  
1:800 Average % 95  
Range % 90-100  
Recovery
The recovery of human ENHO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 87 80-94  
EDTA plasma (n=4) 100 94-104  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
100 2.074 2.099 2.087 1.923  
50 1.798 1.765 1.782 1.618  
25 1.397 1.384 1.391 1.227  
12.5 0.982 0.943 0.963 0.799  
6.25 0.583 0.596 0.590 0.426  
3.12 0.371 0.391 0.381 0.217  
1.56 0.296 0.281 0.289 0.125  
0 0.162 0.166 0.164    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Citations

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Target Background

Function
(From Uniprot)
Involved in the regulation of glucose homeostasis and lipid metabolism.
Gene References into Functions
  1. Serum adropin concentrations are decreased in Chinese T2DM patients, especially those overweight/obese. Adropin, associated with glucolipid homeostasis and insulin sensitivity, may implicate in the pathogenesis of T2DM. PMID: 29669965
  2. The highest adropin concentration was found in patients with P-Ch C (11.7+/-5.7 ng/ml) cirrhosis. PMID: 30260179
  3. Data suggest that serum and follicular fluid levels of adropin are down-regulated in women with PCOS (polycystic ovary syndrome) compared with control subjects; also, follicular fluid levels of adropin are lower than serum levels of adropin. PMID: 28937295
  4. significant association between maternal and umbilical adropin levels and the presence and severity of preeclampsia PMID: 28672759
  5. Adropin is coupled with biological clock activity. In mouse liver, ENHO expression has a diurnal rhythm that peaks at the end of maximal nutrient intake in the dark. The nuclear receptors ROR-alpha/gamma and Rev-erb may couple adropin synthesis with circadian rhythms in carbohydrate and lipid metabolism.In humans, associations between plasma adropin concentrations and LDL-C suggest a link with hepatic lipid metabolism. PMID: 29331507
  6. An increase in maternal serum adropin level was found in preeclampsia. PMID: 28501281
  7. Analysis of midluteal endometrial biopsies revealed an inverse correlation between endometrial EOGT and ENHO expression and body mass index. Obesity impairs the EOGT-adropin axis in decidual cells, which in turn points toward a mechanistic link between metabolic disorders and adverse pregnancy outcome. PMID: 29244071
  8. Serum adropin concentrations are negatively associated with renal function. PMID: 27546995
  9. High adropin expression is associated with polycystic ovary syndrome. PMID: 26969461
  10. There were no significant differences among the groups of systemic sclerosis (SSc) and controls in terms of ENHO gene expressions. There was no significant difference between the limited and diffuse cutaneous subtypes of SSc in terms of serum adropin level and ENHO gene expression. Moreover, serum adropin level and ENHO gene expression were not associated with the disease activity and severity indexes. PMID: 27079850
  11. Enho mutations plays a critical role in activating endothelial cells during neutrophil recruitment and neutrophil-endothelium cell interactions under IL-1 and TNF-alpha-induced vascular inflammation and increases susceptibility to MPOANCA associated lung injury. PMID: 27333037
  12. In HD patients, lower plasma adropin concentration is associated with dyslipidemia. Major homozygosity of RXRA seems to have an opposite effect on plasma adropin compared with that of ENHO rs2281997 PMID: 27449397
  13. Data suggest that serum adropin (ENHO) levels in normal, overweight, and obese adults negatively correlate with vascular stiffness (using common carotid artery) and adiposity (using abdominal visceral fat), and positively correlate with plasma nitric oxide levels (using nitrite/nitrate) and cardiorespiratory fitness; aerobic exercise training up-regulates serum adropin. PMID: 27897440
  14. The adropin levels of metabolic syndrome, obesity, and control groups also showed no difference PMID: 26226125
  15. Circulating adropin levels were determined lower in patients with endometrial cancer than in a control group. PMID: 26172926
  16. Lipids originating either from the diet or from endogenous production appear to positively affect plasma adropin concentrations in humans. PMID: 26435060
  17. Serum adropin levels were significantly lower in obese children; however, there was no correlation between serum adropin levels and blood pressure variables. PMID: 26030787
  18. Adropin as a novel energy factor likely has the ability to regulate blood pressure PMID: 25913544
  19. Serum adropin level was negatively correlated with carotid beta-stiffness and positively correlated with plasma NOx level and cardiorespiratory fitness PMID: 26371163
  20. Adropin levels are lower in patients with late saphenous vein graft occlusion and these reduced adropin levels, together with other factors, may lead to saphenous vein graft occlusion PMID: 25282140
  21. decreased serum adropin levels are associated with the presence of acute myocardial infarction in coronary artery disease patients PMID: 24731968
  22. Assessment of serum adropin concentrations may provide a reliable indicator of fatty liver disease in obese adolescents PMID: 24468600
  23. cord blood adropin levels were positively correlated with gestational age and placental weight but not with other fetal growth parameters. PMID: 24284417
  24. Plasma adropin levels were found to be a new marker for diagnosing endothelial dysfunction in type 2 diabetes mellitus. PMID: 24113736
  25. Release of adropin in the fed condition regulates fuel selection in skeletal muscle, promoting glucose oxidation over fat oxidation. The molecular mechanisms of adropin's effects involve acetylation (suggesting inhibition) of the transcriptional co-activator PGC1alpha, reducing PDK4 and CPT1B activity. Increased PGC1alpha acetylation by adropin may be mediated by inhibiting Sirtuin-1 (SIRT1), a PGC1alpha deacetylase. PMID: 24848071
  26. The mean maternal and cord serum adropin in a gestational diabetes mellitus group were significantly lower than those of control women (P=0.01 and P<0.001, respectively). PMID: 23314506
  27. Adropin is an independent risk factor for cardiac syndrome X (CSX). PMID: 23356444
  28. Although males exhibit higher adropin levels that are reduced by obesity, aging and markers of insulin resistance are associated with low plasma adropin irrespective of sex. PMID: 22872690
  29. Plasma adropin levels were examined in 45 men and 85 woman. Adropin levels are higher in men than woman. Obesity is associated with low adropin levels in men. Aging and metabolic risk factors are associated with low adropin levels, irrespective of sex. PMID: 22872690
  30. Plasma adropin levels are regulated dietary macronutrients increasing with dietary fat content. Fasting suppresses plasma adropin. Adropin's actions are required for preventing insulin resistance, dyslipidemia and impaired glucose tolerance. PMID: 22318315
  31. a potential endothelial protective role of adropin that is likely mediated via upregulation of endothelial NO synthase expression through the VEGFR2-phosphatidylinositol 3-kinase-Akt and VEGFR2-extracellular signal regulated kinase 1/2 pathways. PMID: 20837912
  32. Adropin has an endothelial protective function mediated via upregulation of eNOS expression through the VEGFR2-PI3K-Akt and VEGFR2-ERK1/2 pathways. Adropin therapy may thus be useful for limiting diseases characterized by endothelial dysfunction. PMID: 20837912
  33. Adropin is the name given to the secreted peptide encoded by the ORF in C9orf165. In mice, it is abundant in liver where it is regulated by dietary macronutrients. Adropin regulates expression of genes involved in lipogenesis and adipogenesis. PMID: 19041763

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Subcellular Location Secreted
Tissue Specificity Expressed in liver and brain.
Database Links

HGNC: 24838

KEGG: hsa:375704

STRING: 9606.ENSP00000382675

UniGene: Hs.522085

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