Human Adropin(ENHO) ELISA kit

Code CSB-EL007669HU
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q6UWT2
Lead Time 7-14 working days
Abbreviation ENHO
Target Name energy homeostasis associated
Alias C9orf165, UNQ470, GAAI470|adropin
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 pg/mL-100 pg/mL
Sensitivity 0.39 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 24838
MGI 1916888
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ENHO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 94  
Range % 88-99  
1:200 Average % 90  
Range % 84-95  
1:400 Average % 98  
Range % 91-99  
1:800 Average % 95  
Range % 90-100  
Recovery
The recovery of human ENHO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 87 80-94  
EDTA plasma (n=4) 100 94-104  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
100 2.074 2.099 2.087 1.923  
50 1.798 1.765 1.782 1.618  
25 1.397 1.384 1.391 1.227  
12.5 0.982 0.943 0.963 0.799  
6.25 0.583 0.596 0.590 0.426  
3.12 0.371 0.391 0.381 0.217  
1.56 0.296 0.281 0.289 0.125  
0 0.162 0.166 0.164    
References
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Function Involved in the regulation of glucose homeostasis and lipid metabolism.
Subcellular Location Secreted
Tissue Specificity Expressed in liver and brain.
Database Links

HGNC: 24838

KEGG: hsa:375704

STRING: 9606.ENSP00000382675

UniGene: Hs.522085

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