Code | CSB-EL011620HU |
Size | 96T,5×96T,10×96T |
Trial Size | ![]() |
Have Questions? | Leave a Message or Start an on-line Chat |
Description |
The human interleukin-36 gamma (IL36γ) ELISA kit is suitable for quantitatively determining the human IL36γ complex in multiple samples, including serum, plasma, tissue homogenates, or cell culture supernates. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify human IL36γ levels in the sample. The amount of synthesized colored product is positively related to the analyte of interest in the sample. IL36γ, also called IL1F9, is an IL-1 family cytokine involved in polarizing Type-1 immune responses. IL36G can be induced in keratinocytes, bronchial epithelia, brain tissues, and macrophages and is regarded as an "alarmin" in damaged tissue. Chenyang Dai et al. demonstrated that the IL-36γ/IL-36R axis plays a protective role in Candida albicans (CA) keratitis through upregulating AMPs and downregulating the inflammatory response and consequently improves the outcome of CA keratitis. |
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Target Name | interleukin 1 family, member 9 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Alternative Names | IL 1 epsilon ELISA Kit; IL 1 related protein 2 ELISA Kit; IL 1(EPSILON) ELISA Kit; IL 1F9 ELISA Kit; IL 1H1 ELISA Kit; IL 1RP2 ELISA Kit; IL-1 epsilon ELISA Kit; IL-1-related protein 2 ELISA Kit; IL-1F9 ELISA Kit; IL-1H1 ELISA Kit; IL-1RP2 ELISA Kit; IL1E ELISA Kit; Il1f9 ELISA Kit; IL1F9_HUMAN ELISA Kit; IL1H1 ELISA Kit; IL1RP2 ELISA Kit; IL36 gamma ELISA Kit; IL36G ELISA Kit; Interleukin 1 epsilon ELISA Kit; Interleukin 1 family member 9 ELISA Kit; Interleukin 1 homolog 1 ELISA Kit; Interleukin 1 related protein 2 ELISA Kit; Interleukin 36 gamma ELISA Kit; Interleukin-1 epsilon ELISA Kit; Interleukin-1 family member 9 ELISA Kit; Interleukin-1 homolog 1 ELISA Kit | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Abbreviation | IL1F9 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Uniprot No. | Q9NZH8 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Species | Homo sapiens (Human) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sample Types | serum, plasma, cell culture supernates, tissue homogenates | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detection Range | 78 pg/mL-5000 pg/mL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sensitivity | 19.5 pg/mL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Assay Time | 1-5h | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sample Volume | 50-100ul | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detection Wavelength | 450 nm | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Research Area | Immunology |
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Assay Principle | quantitative | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Measurement | Sandwich | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Precision |
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Linearity |
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Recovery |
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Typical Data |
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ELISA Data Analysis | Watch ELISA data processing video & download Curve Expert if needed | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Troubleshooting and FAQs |
ELISA kit FAQs | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Storage | Store at 2-8°C. Please refer to protocol. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Lead Time | 3-5 working days |
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Function (From Uniprot) |
Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T-cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1; also stimulates its own expression and that of the prototypic cutaneous proinflammatory parameters TNF-alpha, S100A7/psoriasin and inducible NOS. May play a role in proinflammatory responses during particular neutrophilic airway inflammation: activates mitogen-activated protein kinases and NF-kappa B in primary lung fibroblasts, and stimulates the expression of IL-8 and CXCL3 and Th17 chemokine CCL20 in lung fibroblasts. May be involved in the innate immune response to fungal pathogens, such as Aspergillus fumigatus.
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Gene References into Functions |
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Subcellular Location | Cytoplasm. Secreted. |
Protein Families | IL-1 family |
Tissue Specificity | Highly expressed in tissues containing epithelial cells: skin, lung, stomach and esophagus. Expressed in bronchial epithelial. In skin is expressed only in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. Up-regulated in lesional ps |
Database Links |
HGNC: 15741 OMIM: 605542 KEGG: hsa:56300 STRING: 9606.ENSP00000259205 UniGene: Hs.211238 |