Human Interleukin-36 gamma(IL1F9) ELISA kit

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Target Background

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Description

The human interleukin-36 gamma (IL36γ) ELISA kit is suitable for quantitatively determining the human IL36γ complex in multiple samples, including serum, plasma, tissue homogenates, or cell culture supernates. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify human IL36γ levels in the sample. The amount of synthesized colored product is positively related to the analyte of interest in the sample.

IL36γ, also called IL1F9, is an IL-1 family cytokine involved in polarizing Type-1 immune responses. IL36G can be induced in keratinocytes, bronchial epithelia, brain tissues, and macrophages and is regarded as an "alarmin" in damaged tissue. Chenyang Dai et al. demonstrated that the IL-36γ/IL-36R axis plays a protective role in Candida albicans (CA) keratitis through upregulating AMPs and downregulating the inflammatory response and consequently improves the outcome of CA keratitis.

Target Name

interleukin 1 family, member 9

Alternative Names

IL 1 epsilon ELISA Kit; IL 1 related protein 2 ELISA Kit; IL 1(EPSILON) ELISA Kit; IL 1F9 ELISA Kit; IL 1H1 ELISA Kit; IL 1RP2 ELISA Kit; IL-1 epsilon ELISA Kit; IL-1-related protein 2 ELISA Kit; IL-1F9 ELISA Kit; IL-1H1 ELISA Kit; IL-1RP2 ELISA Kit; IL1E ELISA Kit; Il1f9 ELISA Kit; IL1F9_HUMAN ELISA Kit; IL1H1 ELISA Kit; IL1RP2 ELISA Kit; IL36 gamma ELISA Kit; IL36G ELISA Kit; Interleukin 1 epsilon ELISA Kit; Interleukin 1 family member 9 ELISA Kit; Interleukin 1 homolog 1 ELISA Kit; Interleukin 1 related protein 2 ELISA Kit; Interleukin 36 gamma ELISA Kit; Interleukin-1 epsilon ELISA Kit; Interleukin-1 family member 9 ELISA Kit; Interleukin-1 homolog 1 ELISA Kit

Abbreviation

IL1F9

Uniprot No.

Q9NZH8

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

78 pg/mL-5000 pg/mL

Sensitivity

19.5 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IL1F9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	82-95
1:2	Average %	96
	Range %	90-101
1:4	Average %	97
	Range %	92-106
1:8	Average %	95
	Range %	85-99

Recovery

The recovery of human IL1F9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	86-98
EDTA plasma (n=4)	92	88-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
5000	2.052	1.952	2.002	1.898
2500	1.611	1.497	1.554	1.450
1250	1.242	1.204	1.223	1.119
625	0.814	0.757	0.786	0.682
312	0.583	0.538	0.561	0.457
156	0.313	0.289	0.301	0.197
78	0.201	0.199	0.200	0.096
0	0.107	0.101	0.104

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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Target Background

Function (From Uniprot)	Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T-cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1; also stimulates its own expression and that of the prototypic cutaneous proinflammatory parameters TNF-alpha, S100A7/psoriasin and inducible NOS. May play a role in proinflammatory responses during particular neutrophilic airway inflammation: activates mitogen-activated protein kinases and NF-kappa B in primary lung fibroblasts, and stimulates the expression of IL-8 and CXCL3 and Th17 chemokine CCL20 in lung fibroblasts. May be involved in the innate immune response to fungal pathogens, such as Aspergillus fumigatus.
Gene References into Functions	serum IL-36gamma levels were higher in active systemic lupus erythematosus patients and correlated with disease activity and arthritis PMID: 29571080 Cathepsin S was identified as the major IL-36gamma-activating protease expressed in epithelial cells. PMID: 28289191 enhanced expression of IL-36gamma was observed in plasma and bronchoalveolar lavage fluid of patients with acute respiratory distress syndrome because of bacterial pneumonia PMID: 28176791 IL-36-mediated IL-6 and CXCL8 production in human lung fibroblasts and bronchial epithelial cells may be involved in pulmonary inflammation especially caused by bacterial or viral infections. PMID: 28869889 With a focus on the skin as a target for microbial and viral invasion, the current knowledge of IL-36: IL-36alpha, IL-36beta and IL-36gamma, functions is reviewed. One physiological function of the IL-36smay be to counteract microbial immune evasion. [Review] PMID: 28811383 IL-36gamma inhibits differentiation and induces inflammation of keratinocyte via Wnt signaling pathway in psoriasis. PMID: 28924372 IL-36gamma-stimulated endothelial cells secrete the proinflammatory chemokines IL-8, CCL2 and CCL20. PMID: 27673278 skin injury increases IL-36gamma via the activation of TLR3-SLUG-VDR axis and IL-36gamma induces REG3A to promote wound healing PMID: 28774595 Autocrine and Paracrine Regulation of Keratinocyte Proliferation through a Novel Nrf2-IL-36gamma Pathway PMID: 27183581 Here the authors show that Mycobacterium tuberculosis infection of macrophages induces IL-36gamma production in a 2-stage-regulated fashion. PMID: 27389350 IL-36gamma, a member of the IL-1 superfamily, is involved in host defense and contributes to proinflammatory responses and development of inflammatory diseases. PMID: 27853811 IL-36gamma is significantly more strongly expressed in the epidermis of patients with psoriasis-based erythroderma than in other inflammatory skin diseases. PMID: 26524325 Study shows that plasma concentrations of IL-36alpha and IL-36gamma are overexpressed in active systemic lupus erythematosus patients and that IL-36alpha has a substantial pro-inflammatory effect through regulation of IL-6 and CXCL8 production. PMID: 26516833 IRF6 is likely to promote inflammation to P. gingivalis through its regulation of IL-36gamma. PMID: 26819203 Findings indicate that Interleukin (IL)-1beta-induced interleukin 36 gamma (IL-36gamma) expression is mediated by the activation of transcriptional factors, NF-kappaB p65 and AP-1 (c-jun). PMID: 26562662 IL36G was identified as strong regulators of skin pathology in both lesional and non-lesional skin samples. PMID: 25897967 Decreased Langerhans cell responses to IL36G: altered innate immunity in patients with recurrent respiratory papillomatosis PMID: 24950037 IL-36gamma expression inversely correlated with the progression of human melanoma and lung cancer. PMID: 26321222 IL-36gamma is a valuable biomarker in psoriasis patients, both for diagnostic purposes and measurement of disease activity during the clinical course PMID: 25525775 CAMP induces IL-36gamma expression leading to initiation of skin inflammation and occasional exacerbations of psoriasis. PMID: 25305315 IL-36 promotes myeloid cell infiltration, activation, and inflammatory activity in skin PMID: 24829417 This is the first report of extracellular release of endogenous IL-36gamma through pyroptosis suggesting a function of IL-36gamma as an alarmin. PMID: 22318382 Data presented herein shed further light on involvement of T-bet in innate immunity and suggest that IL-36gamma, besides IFNgamma, may contribute to functions of this transcription factor in immunopathology. PMID: 23095752 Interleukin-36 (IL-36) ligands require processing for full agonist (IL-36alpha, IL-36beta, and IL-36gamma) or antagonist (IL-36Ra) activity PMID: 21965679 Regulation and function of the IL-1 family cytokine IL-1F9 in human bronchial epithelial cells. PMID: 20870894 Expression of IL-1F9 is increased in human plaque psoriasis skin and is overexpressed in a transgenic mouse psoriasis model. PMID: 21242515 IL-1F6 and IL-1F8, in addition to IL-1F9, activate the pathway leading to NF-kappaB in an IL-1Rrp2-dependent manner in Jurkat cells PMID: 14734551 This is the first report of IL-1 genotype association with the inflammation of skeletal muscle following acute resistance exercise that may potentially affect the adaptations to chronic resistance exercise. PMID: 15331687 This report demonstrates expression of IL1F9 by bronchial epithelial cells induced by pro-inflammatory stimuli, suggesting a function of this molecule in airway inflammation. PMID: 15701729 Show More Hide All
Subcellular Location	Cytoplasm. Secreted.
Protein Families	IL-1 family
Tissue Specificity	Highly expressed in tissues containing epithelial cells: skin, lung, stomach and esophagus. Expressed in bronchial epithelial. In skin is expressed only in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. Up-regulated in lesional ps
Database Links	HGNC: 15741 OMIM: 605542 KEGG: hsa:56300 STRING: 9606.ENSP00000259205 UniGene: Hs.211238

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Check Code：

Code	CSB-EL011620HU
Size	96T,5×96T,10×96T
Trial Size
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.