Human Interleukin-36 gamma(IL1F9) ELISA kit

Code CSB-EL011620HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


The human interleukin-36 gamma (IL36γ) ELISA kit is suitable for quantitatively determining the human IL36γ complex in multiple samples, including serum, plasma, tissue homogenates, or cell culture supernates. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify human IL36γ levels in the sample. The amount of synthesized colored product is positively related to the analyte of interest in the sample.

IL36γ, also called IL1F9, is an IL-1 family cytokine involved in polarizing Type-1 immune responses. IL36G can be induced in keratinocytes, bronchial epithelia, brain tissues, and macrophages and is regarded as an "alarmin" in damaged tissue. Chenyang Dai et al. demonstrated that the IL-36γ/IL-36R axis plays a protective role in Candida albicans (CA) keratitis through upregulating AMPs and downregulating the inflammatory response and consequently improves the outcome of CA keratitis.

Target Name interleukin 1 family, member 9
Alternative Names IL 1 epsilon ELISA Kit; IL 1 related protein 2 ELISA Kit; IL 1(EPSILON) ELISA Kit; IL 1F9 ELISA Kit; IL 1H1 ELISA Kit; IL 1RP2 ELISA Kit; IL-1 epsilon ELISA Kit; IL-1-related protein 2 ELISA Kit; IL-1F9 ELISA Kit; IL-1H1 ELISA Kit; IL-1RP2 ELISA Kit; IL1E ELISA Kit; Il1f9 ELISA Kit; IL1F9_HUMAN ELISA Kit; IL1H1 ELISA Kit; IL1RP2 ELISA Kit; IL36 gamma ELISA Kit; IL36G ELISA Kit; Interleukin 1 epsilon ELISA Kit; Interleukin 1 family member 9 ELISA Kit; Interleukin 1 homolog 1 ELISA Kit; Interleukin 1 related protein 2 ELISA Kit; Interleukin 36 gamma ELISA Kit; Interleukin-1 epsilon ELISA Kit; Interleukin-1 family member 9 ELISA Kit; Interleukin-1 homolog 1 ELISA Kit
Abbreviation IL1F9
Uniprot No. Q9NZH8
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 19.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of human IL1F9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 89  
Range % 82-95  
1:2 Average % 96  
Range % 90-101  
1:4 Average % 97  
Range % 92-106  
1:8 Average % 95  
Range % 85-99  
The recovery of human IL1F9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 86-98  
EDTA plasma (n=4) 92 88-97  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
5000 2.052 1.952 2.002 1.898  
2500 1.611 1.497 1.554 1.450  
1250 1.242 1.204 1.223 1.119  
625 0.814 0.757 0.786 0.682  
312 0.583 0.538 0.561 0.457  
156 0.313 0.289 0.301 0.197  
78 0.201 0.199 0.200 0.096  
0 0.107 0.101 0.104    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

(From Uniprot)
Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T-cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1; also stimulates its own expression and that of the prototypic cutaneous proinflammatory parameters TNF-alpha, S100A7/psoriasin and inducible NOS. May play a role in proinflammatory responses during particular neutrophilic airway inflammation: activates mitogen-activated protein kinases and NF-kappa B in primary lung fibroblasts, and stimulates the expression of IL-8 and CXCL3 and Th17 chemokine CCL20 in lung fibroblasts. May be involved in the innate immune response to fungal pathogens, such as Aspergillus fumigatus.
Gene References into Functions
  1. serum IL-36gamma levels were higher in active systemic lupus erythematosus patients and correlated with disease activity and arthritis PMID: 29571080
  2. Cathepsin S was identified as the major IL-36gamma-activating protease expressed in epithelial cells. PMID: 28289191
  3. enhanced expression of IL-36gamma was observed in plasma and bronchoalveolar lavage fluid of patients with acute respiratory distress syndrome because of bacterial pneumonia PMID: 28176791
  4. IL-36-mediated IL-6 and CXCL8 production in human lung fibroblasts and bronchial epithelial cells may be involved in pulmonary inflammation especially caused by bacterial or viral infections. PMID: 28869889
  5. With a focus on the skin as a target for microbial and viral invasion, the current knowledge of IL-36: IL-36alpha, IL-36beta and IL-36gamma, functions is reviewed. One physiological function of the IL-36smay be to counteract microbial immune evasion. [Review] PMID: 28811383
  6. IL-36gamma inhibits differentiation and induces inflammation of keratinocyte via Wnt signaling pathway in psoriasis. PMID: 28924372
  7. IL-36gamma-stimulated endothelial cells secrete the proinflammatory chemokines IL-8, CCL2 and CCL20. PMID: 27673278
  8. skin injury increases IL-36gamma via the activation of TLR3-SLUG-VDR axis and IL-36gamma induces REG3A to promote wound healing PMID: 28774595
  9. Autocrine and Paracrine Regulation of Keratinocyte Proliferation through a Novel Nrf2-IL-36gamma Pathway PMID: 27183581
  10. Here the authors show that Mycobacterium tuberculosis infection of macrophages induces IL-36gamma production in a 2-stage-regulated fashion. PMID: 27389350
  11. IL-36gamma, a member of the IL-1 superfamily, is involved in host defense and contributes to proinflammatory responses and development of inflammatory diseases. PMID: 27853811
  12. IL-36gamma is significantly more strongly expressed in the epidermis of patients with psoriasis-based erythroderma than in other inflammatory skin diseases. PMID: 26524325
  13. Study shows that plasma concentrations of IL-36alpha and IL-36gamma are overexpressed in active systemic lupus erythematosus patients and that IL-36alpha has a substantial pro-inflammatory effect through regulation of IL-6 and CXCL8 production. PMID: 26516833
  14. IRF6 is likely to promote inflammation to P. gingivalis through its regulation of IL-36gamma. PMID: 26819203
  15. Findings indicate that Interleukin (IL)-1beta-induced interleukin 36 gamma (IL-36gamma) expression is mediated by the activation of transcriptional factors, NF-kappaB p65 and AP-1 (c-jun). PMID: 26562662
  16. IL36G was identified as strong regulators of skin pathology in both lesional and non-lesional skin samples. PMID: 25897967
  17. Decreased Langerhans cell responses to IL36G: altered innate immunity in patients with recurrent respiratory papillomatosis PMID: 24950037
  18. IL-36gamma expression inversely correlated with the progression of human melanoma and lung cancer. PMID: 26321222
  19. IL-36gamma is a valuable biomarker in psoriasis patients, both for diagnostic purposes and measurement of disease activity during the clinical course PMID: 25525775
  20. CAMP induces IL-36gamma expression leading to initiation of skin inflammation and occasional exacerbations of psoriasis. PMID: 25305315
  21. IL-36 promotes myeloid cell infiltration, activation, and inflammatory activity in skin PMID: 24829417
  22. This is the first report of extracellular release of endogenous IL-36gamma through pyroptosis suggesting a function of IL-36gamma as an alarmin. PMID: 22318382
  23. Data presented herein shed further light on involvement of T-bet in innate immunity and suggest that IL-36gamma, besides IFNgamma, may contribute to functions of this transcription factor in immunopathology. PMID: 23095752
  24. Interleukin-36 (IL-36) ligands require processing for full agonist (IL-36alpha, IL-36beta, and IL-36gamma) or antagonist (IL-36Ra) activity PMID: 21965679
  25. Regulation and function of the IL-1 family cytokine IL-1F9 in human bronchial epithelial cells. PMID: 20870894
  26. Expression of IL-1F9 is increased in human plaque psoriasis skin and is overexpressed in a transgenic mouse psoriasis model. PMID: 21242515
  27. IL-1F6 and IL-1F8, in addition to IL-1F9, activate the pathway leading to NF-kappaB in an IL-1Rrp2-dependent manner in Jurkat cells PMID: 14734551
  28. This is the first report of IL-1 genotype association with the inflammation of skeletal muscle following acute resistance exercise that may potentially affect the adaptations to chronic resistance exercise. PMID: 15331687
  29. This report demonstrates expression of IL1F9 by bronchial epithelial cells induced by pro-inflammatory stimuli, suggesting a function of this molecule in airway inflammation. PMID: 15701729

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Subcellular Location Cytoplasm. Secreted.
Protein Families IL-1 family
Tissue Specificity Highly expressed in tissues containing epithelial cells: skin, lung, stomach and esophagus. Expressed in bronchial epithelial. In skin is expressed only in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. Up-regulated in lesional ps
Database Links

HGNC: 15741

OMIM: 605542

KEGG: hsa:56300

STRING: 9606.ENSP00000259205

UniGene: Hs.211238

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