Human Low affinity immunoglobulin gamma Fc region receptor III-B(FCGR3B) ELISA kit

Code CSB-EL008544HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
Fc fragment of IgG, low affinity IIIb, receptor (CD16b)
Alternative Names
CD16 ELISA Kit; CD16b ELISA Kit; CD16b antigen ELISA Kit; Fc fragment of IgG low affinity IIIb receptor ELISA Kit; Fc gamma receptor IIIb ELISA Kit; Fc gamma RIII ELISA Kit; Fc gamma RIII beta ELISA Kit; Fc gamma RIIIb ELISA Kit; Fc-gamma RIII ELISA Kit; Fc-gamma RIII-beta ELISA Kit; Fc-gamma RIIIb ELISA Kit; FCG3 ELISA Kit; FCG3B_HUMAN ELISA Kit; FCGR3 ELISA Kit; FCGR3B ELISA Kit; FcR 10 ELISA Kit; FcR-10 ELISA Kit; FcR10 ELISA Kit; FcRIII ELISA Kit; FcRIIIb ELISA Kit; IGFR3 ELISA Kit; IgG Fc receptor III 1 ELISA Kit; IgG Fc receptor III-1 ELISA Kit; Low affinity immunoglobulin gamma Fc region receptor III B ELISA Kit; Low affinity immunoglobulin gamma Fc region receptor III-B ELISA Kit
Uniprot No.
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
1.56 ng/mL-100 ng/mL
0.39 ng/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of human FCGR3B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:20 Average % 92  
Range % 86-100  
1:40 Average % 97  
Range % 90-104  
1:80 Average % 95  
Range % 87-99  
1:160 Average % 93  
Range % 90-99  
The recovery of human FCGR3B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 87-98  
EDTA plasma (n=4) 93 90-96  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
100 2.988 2.875 2.932 2.714  
50 2.448 2.396 2.422 2.204  
25 1.678 1.716 1.697 1.479  
12.5 1.025 1.043 1.034 0.816  
6.25 0.670 0.651 0.661 0.443  
3.12 0.473 0.462 0.468 0.250  
1.56 0.321 0.318 0.320 0.102  
0 0.219 0.216 0.218    
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Human FCGR3B ELISA Kit was designed for the quantitative measurement of Human FCGR3B protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.

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Target Background

(From Uniprot)
Receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.
Gene References into Functions
  1. SHP-2 is activated by CD16b crosslinking in neutrophils. PMID: 29137913
  2. FCGR3B deficiency due to copy number variations may predispose to eosinophilic granulomatosis with polyangiitis. PMID: 26684293
  3. The FCGR3B NA1/NA1 genotype is associated with hypersensitivity reactions to adalimumab in Japanese patients with Rheumatoid Arthritis. PMID: 27830964
  4. Data suggest association of FCGR3B deletion with increased risk of rheumatoid arthritis (RA) in a large cohort. PMID: 27995740
  5. Association between Fc gamma Receptor IIIB genetic polymorphisms and susceptibility to severe malaria anemia in children in western Kenya. PMID: 28427365
  6. CD16 and CD35 are important for neutrophil internalization of M. tuberculosis, whereas HIV-1 infection adversely affects opsonophagocytosis. PMID: 27258232
  7. There was no aberration in FCGR3B allele/genotype frequencies in sarcoidosis patients compared to controls. PMID: 26801149
  8. Results showed that susceptibility to systemic lupus erythematosus was associated with the FCGR3B*01 allele, as well as with the FCGR3B*01/*01 and FCGR3B*01/*02 genotypes. PMID: 26946294
  9. FcgammaRIIIB allelic distribution was similar among pediatric Guillain-Barre syndrome patients and controls. PMID: 27064330
  10. data show that cross-linking FcgammaRIIIb is responsible for NET formation by the human neutrophil PMID: 27034964
  11. the results of this meta-analysis indicated that low FCGR3B CN increased susceptibility to autoimmune diseases--{REVIEW} PMID: 26407570
  12. FCGR3B copy number related to systemic lupus and lupus nephritis susceptibility. PMID: 24673810
  13. No relationship was found between the FCGR3B polymorphism and rheumatoid arthritis susceptibility. PMID: 26314337
  14. This study compared the FCGR3B gene frequencies among 230 unrelated healthy Eastern Thai blood donors in Rayong hospital with the previously published studies. PMID: 25626603
  15. FcgammaRIIIb polymorphism is not associated with susceptibility to systemic lupus erythematosus in Brazilian patients PMID: 24896836
  16. in the Indian population, FCGR3B gene copy number varied significantly when compared to other populations of the world. PMID: 25428402
  17. A high copy number of FCGR3B is associated with psoriasis vulgaris in Han Chinese. PMID: 25012234
  18. This suggests that FcgammaRIIIb signals in association with macrophage-1 Ag. PMID: 25024378
  19. The frequencies of FCGR3B*1, FCGR3B*2, and FCGR3B*3 alleles in central Thai blood donors were 0.548, 0.452, and 0.004. PMID: 24205492
  20. Copy number of polymorphisms in FCGR3B gene is associated with ulcerative colitis. PMID: 23917248
  21. The absolute risk of malaria decreased more rapidly with increasing antibody levels for Fc gamma receptor 3B 233AA/AC individuals compared with 233CC children. PMID: 23935200
  22. The FCGR3B NA1/NA2 polymorphism may be associated with susceptibility to aggressive periodontitis. PMID: 23649770
  23. study concludes gene copy number of FcgammaR2C and FcgammaR3B influences IVIG treatment response and predisposes individuals to Kawasaki disease, providing potential insights into understanding the mechanism of the FcgammaR gene family in the IVIG pathway PMID: 23778324
  24. Allele frequencies were determined in the blood donor population as follows: 0.318 for HNA-1a, 0.668 for HNA-1b, 0.014 for HNA-1c, 0.768 for HNA-3a, 0.232 for HNA-3b, 0.882 for HNA-4a, 0.118 for HNA-4b, 0.736 for HNA-5a and 0.264 for HNA-5b. PMID: 23398146
  25. Higher CD16+ cytotoxic NK+T lymphocyte levels predict superior survival outcomes in patients with metastatic carcinoma. PMID: 23281059
  26. Low copy number of the Fc-gamma receptor 3B gene FCGR3B is a risk factor for primary Sjogren's syndrome. PMID: 22942264
  27. Studies indicate beta-defensins (DEFB4, DEFB103, DEFB104), chemokine ligand 3 like 1 (CCL3L1), Fc gamma receptor 3B (FCGR3B), and complement component C4 (C4) for copy number variation in disease association. PMID: 22837109
  28. The FcgammaRIIIB-SH allotype (CTGAAA) containing the 233A-allele (in bold) was associated with protection from malaria. The FcgammaRIIIB-NA2*03 allotype (CTGCGA), a variant of the classical FcgammaRIIIB-NA2 (CTGCAA) was associated with susceptibility to malaria. PMID: 23049979
  29. suggest that impaired immune complex clearance arising from FCGR3B deficiency contributes to the pathology of systemic sclerosis, and FCGR3B copy number variation is a common risk factor for systemic autoimmunity PMID: 22551723
  30. CD14++CD16+ monocytes independently predicted cardiovascular events in subjects referred for elective coronary angiography. PMID: 22999728
  31. Study indicates that a low copy number of the FCGR3B gene is associated with susceptibility to rheumatoid arthritis (RA). PMID: 22309893
  32. The FcgammaRIIIB-NA1 variant, which confers higher affinity for IgG than the NA2 variant, is a determining factor for treatment response. PMID: 22565545
  33. FCGR3B*1, FCGR3B*2 and FCGR3B*3 allele frequencies were respectively 0.347, 0.573 and 0.080 among Tunisians of sub-Saharan origin, 0.379, 0.591 and 0.030 among Tunisian blood donors and 0.318, 0.546 and 0.136 among the group from sub-Saharan Africa. PMID: 22512913
  34. Both ADAM10 and ADAM17 could shed CD16b, but they possess differed preferences. ADAM10 is the main sheddase under stimulation of ionomycin, while ADAM17 is the main sheddase under stimulation of PMA. PMID: 22770404
  35. NA2/NA2 and/or NA2 allele may be risk factors for generalized aggressive periodontitis in the population of South India PMID: 22167032
  36. In this Brazilian FCGR3B*03(+) group we found that the inheritance of FCGR3B*03 took place by a linkage to FCGR3B*02 or to FCGR3B*01. PMID: 21895673
  37. FCGR3B DNA copy number variation is associated with systemic lupus erythematosus risk in afro-caribbeans. PMID: 21296850
  38. Monocyte subsets have specialized roles during inflammation: CD16-positive monocytes might undergo an immediate innate immune response, whereas CD16-negative monocytes could have a major role as antigen presenting cells. PMID: 21937707
  39. Our results suggest that FcgammaRIIIb might not be a susceptibility gene for SLE and lupus nephritis. PMID: 20300756
  40. copy number associated with susceptibility to idiopathic pulmonary fibrosis PMID: 21109729
  41. IL-21 stimulates the production of IL-10 by CD4(+) T-cells. Secreted Il-10 maintains the expression of CD16 on monocytes. PMID: 21227406
  42. the neutrophil responses to stimuli that engage FcGR3B and FcGR2A, namely the phagocytosis of human IgG-opsonized zymosan and the responses to heat-aggregated IgGs PMID: 21123174
  43. FcgammaRIIIb NA1/2 polymorphisms are associated with idiopathic pulmonary fibrosis disease susceptibility but not with disease progression. PMID: 20924590
  44. study highlights the complexity and poor characterization of the FCGR3B gene sequence, indicating that the design and interpretation of genotyping assays based on specific probe sequences must be performed with caution PMID: 20957197
  45. The FCGR3B NA1/NA1 genotype was found in 75% of the patients with infusion reactions and in only 37% of those without (p=0.01), whereas the FCGR3A 176F/V genotype was equally distributed in the patients with or without infusion reactions. PMID: 20980704
  46. FcgammRIIA and FcgammaRIIIB are required for neutrophil mediated dermal epidermal separation in bullous pemphigoid PMID: 20720565
  47. results, obtained by fitting logistic regression models, confirm the association of low copy number of FCGR3B with systemic lupus erythematosus PMID: 20442749
  48. Results of the present study suggest that subjects carrying at least one copy of the FcgammaRIIIb-NA2 allele might be associated with susceptibility to aggressive periodontitis. PMID: 20041976
  49. The primary involvement of Fc gamma R IIIB NA2 allele is more likely involved with disease susceptibility of systemic lupus erythematosus. PMID: 20536598
  50. Significant evidence for an association between copy number and rheumatoid arthritis was observed in the Dutch cohort but not in the two smaller cohorts (New Zealand and United Kingdom populations, respectively). PMID: 20472591

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Subcellular Location
Cell membrane; Lipid-anchor, GPI-anchor. Secreted. Note=Secreted after cleavage.
Tissue Specificity
Expressed specifically by polymorphonuclear leukocytes (neutrophils). Also expressed by stimulated eosinophils.
Database Links

HGNC: 3620

OMIM: 610665

KEGG: hsa:2215

STRING: 9606.ENSP00000294800

UniGene: Hs.372679

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