Code | CSB-E04686h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. |
To assess the linearity of the assay, samples were spiked with high concentrations of human NT-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | ||||
1:1 | Average % | 86 | ||||
Range % | 80-92 | |||||
1:2 | Average % | 95 | ||||
Range % | 91-99 | |||||
1:4 | Average % | 95 | ||||
Range % | 92-104 | |||||
1:8 | Average % | 94 | ||||
Range % | 87-98 |
The recovery of human NT-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 95 | 89-99 | ||||
EDTA plasma (n=4) | 94 | 90-98 |
| |||||||
ng/ml | OD1 | OD2 | Average | Corrected | |||
80 | 2.181 | 2.081 | 2.131 | 1.999 | |||
40 | 1.356 | 1.256 | 1.306 | 1.174 | |||
20 | 0.705 | 0.685 | 0.695 | 0.563 | |||
10 | 0.446 | 0.436 | 0.441 | 0.309 | |||
5 | 0.270 | 0.260 | 0.265 | 0.133 | |||
2.5 | 0.230 | 0.224 | 0.227 | 0.095 | |||
1.25 | 0.187 | 0.184 | 0.186 | 0.054 | |||
0 | 0.132 | 0.131 | 0.132 |
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The Human Neurotrophin-3 (NT-3) ELISA KIT is the perfect tool for accurate and reliable detection of Neurotrophin-3 protein levels in human serum, plasma, and cell culture supernates.
NT-3, a protein encoded by the NTF3 gene in humans, plays a crucial role in the development and maintenance of the nervous system, making it a key research target in the field of neuroscience. Our ELISA kit offers a sensitive and specific way to quantitatively measure NT-3 levels, providing researchers with valuable insights into the role of this protein in various biological processes.
With a detection range of 1.25 ng/mL-80 ng/mL and a sensitivity of 0.31 ng/mL, this kit delivers accurate and reproducible results in just 1-5 hours. The assay principle is based on a sandwich ELISA method, ensuring high specificity and minimal interference from other proteins in the sample.
The kit is easy to use, with a sample volume of 50-100ul, and a detection wavelength of 450 nm. Our Human Neurotrophin-3 (NT-3) ELISA KIT is perfect for researchers in the field of neuroscience looking to quantify Neurotrophin-3 levels in human samples accurately. With more than three citations, our kit has been proven to deliver reliable and consistent results, making it a trusted choice for researchers worldwide.
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