Human Proto-oncogene Wnt-3(WNT3) ELISA kit

Code CSB-EL026135HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human WNT3 ELISA Kit was designed for the quantitative measurement of Human WNT3 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

Target Name wingless-type MMTV integration site family, member 3
Alternative Names Int 4 ELISA Kit; INT4 ELISA Kit; MGC131950 ELISA Kit; MGC138321 ELISA Kit; MGC138323 ELISA Kit; Proto-oncogene Int-4 homolog ELISA Kit; Proto-oncogene Wnt-3 ELISA Kit; wingless type MMTV integration site family member 3 ELISA Kit; Wnt 3 proto oncogene protein ELISA Kit; WNT 3 proto oncogene protein precursor ELISA Kit; wnt3 ELISA Kit; WNT3_HUMAN ELISA Kit
Abbreviation WNT3
Uniprot No. P56703
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Stem Cells
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human WNT3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %93
Range %89-97
1:2Average %105
Range %100-110
1:4Average %90
Range %85-94
1:8Average %84
Range %80-88
Recovery
The recovery of human WNT3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8983-94
EDTA plasma (n=4)9893-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.246 2.301 2.274 2.058
51.705 1.642 1.674 1.458
2.51.244 1.320 1.282 1.066
1.250.905 0.873 0.889 0.673
0.6250.541 0.542 0.542 0.326
0.3120.435 0.467 0.451 0.235
0.1560.312 0.325 0.319 0.103
00.215 0.216 0.216
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Required for normal gastrulation, formation of the primitive streak, and for the formation of the mesoderm during early embryogenesis. Required for normal formation of the apical ectodermal ridge. Required for normal embryonic development, and especially for limb development.
Gene References into Functions
  1. Demonstrate a significant change in miRNA profile dependent on the assisted reproductive technology outcome affecting Wnt pathway. PMID: 28971890
  2. High expression of WNT3 is associated with hepatocellular carcinoma. PMID: 28586038
  3. Our data demonstrated an important interdependence between TGF-beta and Wnt/beta-catenin pathways inducing EMT in HER2-overexpressing breast cancer cells. Twist served as a linkage between the two pathways during TGF-beta-induced EMT. A83-01 could inhibit the TGF-beta-initiated pathway interactions and enhance HER2-cells response to trastuzumab treatment PMID: 28337662
  4. YAP maintains human embryonic stem cells pluripotency by preventing WNT3 expression in response to Activin, thereby blocking a direct route to embryonic cardiac mesoderm formation. PMID: 29269485
  5. Wnt3 is associated with cholesterol-dependent domains PMID: 27463143
  6. analysis of WNT3 expression in CLL patients demonstrated that (i) untreated patients with more aggressive disease (with a notable exception of patients with 11q deletion) express less WNT3, (ii) WNT3 declines with disease progression in a significant proportion of patients and (iii) low WNT3 was identified as a strong independent marker indicating shorter treatment-free survival in CLL patients with IGHV mutation. PMID: 27651098
  7. PRKX, WNT3 and WNT16 genes, belonging to the WNT signaling pathway, are involved in the tumorigenic process of nodular basal cell carcinoma PMID: 27630294
  8. Two SNPs (rs3809857 and rs9890413) in the WNT3 gene were subjected to case-control and case-parent analysis PMID: 26505415
  9. WNT3 and membrane-associated beta-catenin regulate trophectoderm lineage differentiation in human blastocysts. PMID: 26108805
  10. WNT3 involvement in human bladder exstrophy and cloaca development in zebrafish PMID: 26105184
  11. Genome association study shows a highly conserved 32 kb intergenic region containing regulatory elements between WNT3 and WNT9B in patients with classic bladder exstrophy. PMID: 24852367
  12. mRNA level of WNT3 in hESCs correlates with their definitive ectoderm differentiation efficiency. PMID: 24052941
  13. Data show that the WNT/beta-CATENIN signaling cascade components, including WNT ligands WNT3 and WNT5A, is crucial for early odontogenesis. PMID: 24647585
  14. WNT3 inhibits cerebellar granule neuron progenitor proliferation and medulloblastoma formation via MAPK activation. PMID: 24303070
  15. Colorectal tumors express elevated levels of Wnt3 and GPR177. PMID: 24162018
  16. WNT3 and WNT5B are critical factors, secreted from mesenchymal cancer cells, for instigating the epithelial cancer cell invasion. PMID: 24344732
  17. Knockdown of Wnt3 by siRNA restored cytoplasmic expression of beta-catenin. PMID: 23071104
  18. The apoptotic index was significantly lower in high-Wnt3 tumors than in low-Wnt3 tumors (P=0.0245). PMID: 22070884
  19. study confirmed the involvement of polymorphisms in the WNT3 gene in NCL/P aetiology in the tested population. PMID: 22288914
  20. The findings of a significant association between lip and/or palate clefts and two markers in the WNT3 and COL11A2 genes were the most consistent and were observed in all groups analysed and stratified. PMID: 22112025
  21. SNP rs415430 in the WNT3 gene was not associated with the risk of development of Parkinson disease PMID: 21789800
  22. Extracellular domain of FZD7 (sFZD7) was tested for its functional activity to interact with Wnt3, its ability to inhibit Wnt3-mediated signaling. PMID: 21314951
  23. Abnormalities of the Wnt3/3a pathway (located in the apical ectodermal ridge) include tetra-amelia and loss of the distal phalanges/nails. PMID: 20709709
  24. Individuals carrying variant alleles in WNT3 presented an increased risk for cleft lip/palate (p = 0.0003; OR, 1.61; 95% CI, 1.29-2.02) in the Brazilian population studied. PMID: 20890934
  25. Results demonstrated significant up-regulation of WNT-3, WNT-4, WNT-5B, WNT-7B, WNT-9A, WNT-10A, and WNT-16B in patients with CLL compared to normal subjects. PMID: 19863181
  26. Regulation of WNT3 and WNT3A mRNAs in human cancer cell lines NT2, MCF-7, and MKN45 PMID: 11788904
  27. Wnt3 plays an important role in regulating characteristics and activity of stromal cells. PMID: 15588944
  28. The Wnt-3a triggers the interaction of LRP6 with caveolin and promotes recruitment of Axin to LRP6 phosphorylated by glycogen synthase kinase-3beta and that caveolin thereby inhibits the binding of beta-catenin to Axin. PMID: 16890161
  29. an internally truncated LRP5 receptor is strongly implicated in deregulated activation of the WNT/beta-catenin signaling pathway in hyperparathyroid tumors PMID: 18044981
  30. The results indicated that HBx induction in the CCL13-HBx stable cell line downregulated Wnt-3/beta-catenin expression and suppressed cell growth by repressing cell proliferation or triggering cell apoptosis. PMID: 18309246
  31. a functional interaction between Wnt3 and FZD7 leading to activation of the Wnt/beta-catenin signaling pathway in HCC cells and may play a role during hepatocarcinogenesis. PMID: 18313787
  32. CTLA-4 is a direct target of Wnt/beta-catenin signaling and is expressed in human melanoma tumors PMID: 18563180
  33. WNT3 regulates distinct internalization pathways of LRP6 to tune the activation of beta catenin signaling. PMID: 18606139
  34. mantle cell lymphoma highly and consistently expressed Wnt3 and Wnt10. PMID: 18787224
  35. WNT activity regulates the self-renewal of prostate cancer cells with stem cell characteristics independently of androgen receptor activity. PMID: 19365403

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Involvement in disease Tetraamelia syndrome, autosomal recessive (TETAMS)
Subcellular Location Secreted, extracellular space, extracellular matrix. Secreted.
Protein Families Wnt family
Database Links

HGNC: 12782

OMIM: 165330

KEGG: hsa:7473

STRING: 9606.ENSP00000225512

UniGene: Hs.445884

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