Recombinant Human Proto-oncogene Wnt-3(WNT3)

Code CSB-YP026135HU
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Source Yeast
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Code CSB-EP026135HU
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Source E.coli
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Code CSB-EP026135HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP026135HU
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Source Baculovirus
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Code CSB-MP026135HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names WNT3
Uniprot No. P56703
Alternative Names Int 4; INT4; MGC131950; MGC138321; MGC138323; Proto-oncogene Int-4 homolog; Proto-oncogene Wnt-3; wingless type MMTV integration site family member 3; Wnt 3 proto oncogene protein; WNT 3 proto oncogene protein precursor; wnt3; WNT3_HUMAN
Species Homo sapiens (Human)
Expression Region 22-355
Target Protein Sequence GYPIWWSLA LGQQYTSLGS QPLLCGSIPG LVPKQLRFCR NYIEIMPSVA EGVKLGIQEC QHQFRGRRWN CTTIDDSLAI FGPVLDKATR ESAFVHAIAS AGVAFAVTRS CAEGTSTICG CDSHHKGPPG EGWKWGGCSE DADFGVLVSR EFADARENRP DARSAMNKHN NEAGRTTILD HMHLKCKCHG LSGSCEVKTC WWAQPDFRAI GDFLKDKYDS ASEMVVEKHR ESRGWVETLR AKYSLFKPPT ERDLVYYENS PNFCEPNPET GSFGTRDRTC NVTSHGIDGC DLLCCGRGHN TRTEKRKEKC HCIFHWCCYV SCQECIRIYD VHTCK
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Required for normal gastrulation, formation of the primitive streak, and for the formation of the mesoderm during early embryogenesis. Required for normal formation of the apical ectodermal ridge. Required for normal embryonic development, and especially for limb development.
Gene References into Functions
  1. Demonstrate a significant change in miRNA profile dependent on the assisted reproductive technology outcome affecting Wnt pathway. PMID: 28971890
  2. High expression of WNT3 is associated with hepatocellular carcinoma. PMID: 28586038
  3. Our data demonstrated an important interdependence between TGF-beta and Wnt/beta-catenin pathways inducing EMT in HER2-overexpressing breast cancer cells. Twist served as a linkage between the two pathways during TGF-beta-induced EMT. A83-01 could inhibit the TGF-beta-initiated pathway interactions and enhance HER2-cells response to trastuzumab treatment PMID: 28337662
  4. YAP maintains human embryonic stem cells pluripotency by preventing WNT3 expression in response to Activin, thereby blocking a direct route to embryonic cardiac mesoderm formation. PMID: 29269485
  5. Wnt3 is associated with cholesterol-dependent domains PMID: 27463143
  6. analysis of WNT3 expression in CLL patients demonstrated that (i) untreated patients with more aggressive disease (with a notable exception of patients with 11q deletion) express less WNT3, (ii) WNT3 declines with disease progression in a significant proportion of patients and (iii) low WNT3 was identified as a strong independent marker indicating shorter treatment-free survival in CLL patients with IGHV mutation. PMID: 27651098
  7. PRKX, WNT3 and WNT16 genes, belonging to the WNT signaling pathway, are involved in the tumorigenic process of nodular basal cell carcinoma PMID: 27630294
  8. Two SNPs (rs3809857 and rs9890413) in the WNT3 gene were subjected to case-control and case-parent analysis PMID: 26505415
  9. WNT3 and membrane-associated beta-catenin regulate trophectoderm lineage differentiation in human blastocysts. PMID: 26108805
  10. WNT3 involvement in human bladder exstrophy and cloaca development in zebrafish PMID: 26105184
  11. Genome association study shows a highly conserved 32 kb intergenic region containing regulatory elements between WNT3 and WNT9B in patients with classic bladder exstrophy. PMID: 24852367
  12. mRNA level of WNT3 in hESCs correlates with their definitive ectoderm differentiation efficiency. PMID: 24052941
  13. Data show that the WNT/beta-CATENIN signaling cascade components, including WNT ligands WNT3 and WNT5A, is crucial for early odontogenesis. PMID: 24647585
  14. WNT3 inhibits cerebellar granule neuron progenitor proliferation and medulloblastoma formation via MAPK activation. PMID: 24303070
  15. Colorectal tumors express elevated levels of Wnt3 and GPR177. PMID: 24162018
  16. WNT3 and WNT5B are critical factors, secreted from mesenchymal cancer cells, for instigating the epithelial cancer cell invasion. PMID: 24344732
  17. Knockdown of Wnt3 by siRNA restored cytoplasmic expression of beta-catenin. PMID: 23071104
  18. The apoptotic index was significantly lower in high-Wnt3 tumors than in low-Wnt3 tumors (P=0.0245). PMID: 22070884
  19. study confirmed the involvement of polymorphisms in the WNT3 gene in NCL/P aetiology in the tested population. PMID: 22288914
  20. The findings of a significant association between lip and/or palate clefts and two markers in the WNT3 and COL11A2 genes were the most consistent and were observed in all groups analysed and stratified. PMID: 22112025
  21. SNP rs415430 in the WNT3 gene was not associated with the risk of development of Parkinson disease PMID: 21789800
  22. Extracellular domain of FZD7 (sFZD7) was tested for its functional activity to interact with Wnt3, its ability to inhibit Wnt3-mediated signaling. PMID: 21314951
  23. Abnormalities of the Wnt3/3a pathway (located in the apical ectodermal ridge) include tetra-amelia and loss of the distal phalanges/nails. PMID: 20709709
  24. Individuals carrying variant alleles in WNT3 presented an increased risk for cleft lip/palate (p = 0.0003; OR, 1.61; 95% CI, 1.29-2.02) in the Brazilian population studied. PMID: 20890934
  25. Results demonstrated significant up-regulation of WNT-3, WNT-4, WNT-5B, WNT-7B, WNT-9A, WNT-10A, and WNT-16B in patients with CLL compared to normal subjects. PMID: 19863181
  26. Regulation of WNT3 and WNT3A mRNAs in human cancer cell lines NT2, MCF-7, and MKN45 PMID: 11788904
  27. Wnt3 plays an important role in regulating characteristics and activity of stromal cells. PMID: 15588944
  28. The Wnt-3a triggers the interaction of LRP6 with caveolin and promotes recruitment of Axin to LRP6 phosphorylated by glycogen synthase kinase-3beta and that caveolin thereby inhibits the binding of beta-catenin to Axin. PMID: 16890161
  29. an internally truncated LRP5 receptor is strongly implicated in deregulated activation of the WNT/beta-catenin signaling pathway in hyperparathyroid tumors PMID: 18044981
  30. The results indicated that HBx induction in the CCL13-HBx stable cell line downregulated Wnt-3/beta-catenin expression and suppressed cell growth by repressing cell proliferation or triggering cell apoptosis. PMID: 18309246
  31. a functional interaction between Wnt3 and FZD7 leading to activation of the Wnt/beta-catenin signaling pathway in HCC cells and may play a role during hepatocarcinogenesis. PMID: 18313787
  32. CTLA-4 is a direct target of Wnt/beta-catenin signaling and is expressed in human melanoma tumors PMID: 18563180
  33. WNT3 regulates distinct internalization pathways of LRP6 to tune the activation of beta catenin signaling. PMID: 18606139
  34. mantle cell lymphoma highly and consistently expressed Wnt3 and Wnt10. PMID: 18787224
  35. WNT activity regulates the self-renewal of prostate cancer cells with stem cell characteristics independently of androgen receptor activity. PMID: 19365403

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Involvement in disease Tetraamelia syndrome, autosomal recessive (TETAMS)
Subcellular Location Secreted, extracellular space, extracellular matrix. Secreted.
Protein Families Wnt family
Database Links

HGNC: 12782

OMIM: 165330

KEGG: hsa:7473

STRING: 9606.ENSP00000225512

UniGene: Hs.445884

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