Human fibroblast growth factor-23,FGF-23 ELISA Kit

Code CSB-E10113h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q9GZV9
Lead Time 3-5 working days
Abbreviation FGF23
Protein Biological Process 1 Developmental Protein
Target Name fibroblast growth factor 23
Alias ADHR, HPDR2, HYPF, PHPTC, tumor-derived hypophosphatemia inducing factor
Species Homo sapiens (Human)
Protein Biological Process 3 Differentiation
Sample Types serum, cell culture supernates, urine, tissue homogenates
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The product of this gene inhibits renal tubular phosphate transport. This gene was identified by its mutations associated with autosomal dominant hypophosphatemic rickets (ADHR), an inherited phosphate wasting disorder. Abnormally high level expression of this gene was found in oncogenic hypophosphatemic osteomalacia (OHO), a phenotypically similar disease caused by abnormal phosphate metabolism. Mutations in this gene have also been shown to cause familial tumoral calcinosis with hyperphosphatemia.
HGNC 3680
RGD 620178
MGI 1891427
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human FGF-23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 99  
Range % 80-115  
1:2 Average % 100  
Range % 91-110  
1:4 Average % 97  
Range % 92-115  
1:8 Average % 93  
Range % 86-100  
Recovery
The recovery of human FGF-23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
200 2.958 2.986 2.972 2.783  
100 2.461 2.475 2.468 2.279  
50 1.852 1.889 1.871 1.682  
25 1.234 1.286 1.260 1.071  
12.5 0.753 0.786 0.770 0.581  
6.25 0.531 0.556 0.544 0.355  
3.12 0.396 0.416 0.406 0.217  
0 0.192 0.186 0.189    
References
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Function Regulator of phosphate homeostasis. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels. Upregulates EGR1 expression in the presence of KL (By similarity). Acts directly on the parathyroid to decrease PTH secretion (By similarity). Regulator of vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization.
Involvement in disease Hypophosphatemic rickets, autosomal dominant (ADHR); Tumoral calcinosis, hyperphosphatemic, familial (HFTC)
Subcellular Location Secreted
Protein Families Heparin-binding growth factors family
Tissue Specificity Expressed in osteogenic cells particularly during phases of active bone remodeling. In adult trabecular bone, expressed in osteocytes and flattened bone-lining cells (inactive osteoblasts).
Database Links

HGNC: 3680

OMIM: 193100

KEGG: hsa:8074

STRING: 9606.ENSP00000237837

UniGene: Hs.287370

Pathway MAPK signaling pathway
PI3K-Akt signaling pathway
Regulation of actin cytoskeleton
Ras signaling pathway
Rap1 signaling pathway

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