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24T ELISA Kit Trial Size (Only USD$150/ kit)
|Intra-assay Precision (Precision within an assay): CV%<8%
|Three samples of known concentration were tested twenty times on one plate to assess.
|Inter-assay Precision (Precision between assays): CV%<10%
|Three samples of known concentration were tested in twenty assays to assess.
|To assess the linearity of the assay, samples were spiked with high concentrations of human FGF-23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
|The recovery of human FGF-23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
|Average % Recovery
|These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
The human FGF23 ELISA Kit allows for the in vitro quantitative determination of FGF23 concentrations in serum, cell culture supernates, urine, or tissue homogenates. This kit exclusively recognizes human FGF23. FGF23 is a bone-derived hormone that inhibits phosphate reabsorption and vitamin D hormone synthesis in the kidney. FGF23 signals through α-Klotho (KLA) and FGF receptor (FGFR) leading to kinase activation, modulation of phosphate homeostasis, vitamin D levels, and calcium metabolism. Increased FGF23 abundance is responsible for several hereditary and acquired hypophosphatemic rickets disorders or tumor-induced osteomalacia. Deficiency of FGF23 results in elevated phosphate levels, such as in familiar tumor calcinosis.
The detection mechanism of this kit is based on the Sandwich-ELISA technique. FGF23 in the sample is bound to the capture antibody immobilized on the microtiter plate and then sandwiched with a Biotin-labeled FGF23 antibody. The solution color develops into blue after the ordinal addition of HRP-avidin and TMB. The color development is terminated after adding the stop solution, and the color turns from blue to yellow. The color intensity is positively correlated with FGF23 content in the sample. The kit has been quality-controlled with high sensitivity, strong specificity, good linearity, high precision, high recovery, and high lot-to-lot consistency.
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I'd like to know if is possible performed the FGF 23 assay on plasma samples. Only on serum?
I'd like to know if the kit CSB-E10113h recognizes C-terminal fragments or Intact FGF 23