Human fibroblast growth factor-23,FGF-23 ELISA Kit

Instructions
Code CSB-E10113h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name fibroblast growth factor 23
Alternative Names ADHR, HPDR2, HYPF, PHPTC, tumor-derived hypophosphatemia inducing factor
Abbreviation FGF23
Uniprot No. Q9GZV9
Species Homo sapiens (Human)
Sample Types serum, cell culture supernates, urine, tissue homogenates
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human FGF-23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 99  
Range % 80-115  
1:2 Average % 100  
Range % 91-110  
1:4 Average % 97  
Range % 92-115  
1:8 Average % 93  
Range % 86-100  
Recovery
The recovery of human FGF-23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
200 2.958 2.986 2.972 2.783  
100 2.461 2.475 2.468 2.279  
50 1.852 1.889 1.871 1.682  
25 1.234 1.286 1.260 1.071  
12.5 0.753 0.786 0.770 0.581  
6.25 0.531 0.556 0.544 0.355  
3.12 0.396 0.416 0.406 0.217  
0 0.192 0.186 0.189    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Citations

Q&A and Customer Reviews

 Q&A
Q:

I'd like to know if is possible performed the FGF 23 assay on plasma samples. Only on serum?

A:
Thanks for your inquiry.
CSB-E10113h It is recommended to test the serum sample.
Plasma tests are not very effective. It is estimated that the presence of fibrinogen interferes with the test or the content of the plasma samples we collected is too low.Pls let me know if you have any further questions. Thank you.
Q:

I'd like to know if the kit CSB-E10113h recognizes C-terminal fragments or Intact FGF 23

A:
Thanks for your inquiry.
The kit is designed for full-length, pls check the detailed information from this link: https://www.uniprot.org/uniprot/Q9GZV9 Pls let me know if you have any further questions. Thank you.
 Customer Reviews

Target Data

Function Regulator of phosphate homeostasis. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels. Upregulates EGR1 expression in the presence of KL (By similarity). Acts directly on the parathyroid to decrease PTH secretion (By similarity). Regulator of vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization.
Involvement in disease Hypophosphatemic rickets, autosomal dominant (ADHR); Tumoral calcinosis, hyperphosphatemic, familial (HFTC)
Subcellular Location Secreted
Protein Families Heparin-binding growth factors family
Tissue Specificity Expressed in osteogenic cells particularly during phases of active bone remodeling. In adult trabecular bone, expressed in osteocytes and flattened bone-lining cells (inactive osteoblasts).
Database Links

HGNC: 3680

OMIM: 193100

KEGG: hsa:8074

STRING: 9606.ENSP00000237837

UniGene: Hs.287370

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