Mouse Protein CYR61(CYR61) ELISA kit

Instructions
Code CSB-EL006463MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name cysteine-rich, angiogenic inducer, 61
Alternative Names Ccn1 ELISA Kit; Cyr61 ELISA Kit; Igfbp10 ELISA Kit; CCN family member 1 ELISA Kit; 3CH61 ELISA Kit; Cellular communication network factor 1 ELISA Kit; Cysteine-rich angiogenic inducer 61 ELISA Kit; Insulin-like growth factor-binding protein 10 ELISA Kit; IBP-10 ELISA Kit; IGF-binding protein 10 ELISA Kit; IGFBP-10 ELISA Kit; Protein CYR61 ELISA Kit
Abbreviation CYR61
Uniprot No. P18406
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 18.75 pg/mL-1200 pg/mL
Sensitivity 4.68 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CYR61 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:100Average %91
Range %86-99
1:200Average %97
Range %92-104
1:400Average %93
Range %88-98
1:800Average %88
Range %83-94
Recovery
The recovery of mouse CYR61 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9388-98
EDTA plasma (n=4)9692-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
12002.584 2.652 2.618 2.498
6002.175 2.075 2.125 2.005
3001.607 1.603 1.605 1.485
1501.016 1.090 1.053 0.933
750.503 0.524 0.514 0.394
37.50.328 0.355 0.342 0.222
18.750.232 0.229 0.231 0.111
00.120 0.119 0.120  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Background

Function
(From Uniprot)
Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5 (By similarity). CYR61-mediated gene regulation is dependent on heparin-binding (By similarity). Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1 (By similarity). Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-1/beta-5 and cell proliferation through integrin alpha-v/beta-3 (By similarity).
Gene References into Functions
  1. These findings reveal a critical role for CCN1 in restoring mucosal homeostasis after intestinal injury PMID: 25807183
  2. CYR61 is upregulated in acute lung injury (ALI) and CYR61 overexpression exacerbates ALI in mice. PMID: 25713320
  3. CYR61 silencing can blunt the malignant behavior of osteosarcoma tumor cells by limiting primary tumor growth and dissemination process. PMID: 25065593
  4. Knock-in mice expressing a CCN1 mutant that is unable to bind alphavbeta5/alphavbeta3 were impaired in ductular reaction. CCN1, acting through its alphav beta3/alphav beta5 binding site, is critical for survival after bile duct ligation. PMID: 25822023
  5. Findings reveal that CCN1 plays a novel role in pathogen clearance; increasing macrophage infiltration and activation resulting in increased virus clearance in tumors. PMID: 24895995
  6. Extracellular Cyr61 convergence with growth factor signaling and integrin/FAK signaling is a new concept of growth factor-induced cell migration. PMID: 25623072
  7. These findings implicate CCN1 as a mediator of cardioprotection induced by G-protein coupled receptor agonists that activate RhoA/MRTF-A signaling. PMID: 25106095
  8. Our results here reveal a novel role of Cyr61 in the pathogenesis of rheumatoid arthritis PMID: 24517278
  9. Findings suggest that that cysteine-rich 61 protein (cyr61) is expressed in airway epithelial cells and has a positive correlation with eotaxin and number of airway infiltrating eosinophils. PMID: 24142710
  10. It provides evidence that CCN1 is released from activated platelets, suggesting that platelets constitute a novel source for CCN1 release to the circulation during sepsis. PMID: 24430538
  11. Binding of CYR61 to LFA-1, ICAM-1 and integrin alpha6 supports the adhesion of thymic epithelial cells and T-cell. PMID: 24280864
  12. these data reveal that de novo Cyr61 in the extracellular matrix bridges LPA and integrin pathways, which in turn, activate FAK, leading to cell migration. PMID: 24371135
  13. CCN1 protein expression is increased in aging skeletal muscle. PMID: 24196529
  14. The aim of the study was to evaluate CCN1 protein abundance and localization in the kidney of IL-6-deficient C57BL/6J (IL-6 KO) mice. PMID: 23690222
  15. Degradome products of the matricellular protein CCN1 as modulators of pathological angiogenesis in the retina. PMID: 23798676
  16. role of Cyr61 in obstructive kidney fibrosis PMID: 23457573
  17. the potential roles of CCN1 in the inflammation and macrophage infiltration of nonalcoholic fatty liver disease ( PMID: 23071295
  18. the matricellular protein CCN1/Cyr61 is a critical regulator of Sonic Hedgehog in pancreatic carcinogenesis PMID: 23027863
  19. The expressions of Cyr61, MMP-2, and VEGF at the levels of mRNA and protein were decreased in the QYHJ groups. PMID: 21382954
  20. a novel, VEGF-independent, clinically relevant, pro-angiogenic factor, CYR61, that is a transcriptional target of Hh-GLI signaling PMID: 22056874
  21. Cyr61 expression increases with tumor grade in human osteosarcoma and demonstrate that cyr61 silencing inhibits in vitro osteosarcoma cell invasion and migration as well as in vivo lung metastases in mice. PMID: 21312266
  22. newly identified functions of CCN1 suggest its possible therapeutic utility in ischemic retinopathy. PMID: 21212276
  23. extracellular matrix protein CCN1 dictates TNFalpha and FasL cytotoxicity PMID: 21153366
  24. Expression of Cyr61 is induced in fibroblasts through plasmin-induced activation of the protease-activated receptor-1 signaling pathway. PMID: 12231560
  25. These findings establish CYR61 as a novel and essential regulator of vascular development. PMID: 12446788
  26. Pressure overload, ischemia, & neurohormonal factors induce myocardial expression of CCN1, a potent proangiogenic factor. CCN1 may play an important role in the adaptation of the heart to cardiovascular stress. PMID: 15117851
  27. results identify a novel and functionally important binding site for integrin alphavbeta3 and provide a new approach for dissecting alphavbeta3-specific CCN1 functions both in cultured cells and in the organism PMID: 15308622
  28. CCN1 acts through integrin alpha6beta1 and heparan sulfate proteoglycans (HSPGs) to promote specific functions in fibroblasts. PMID: 15322081
  29. Ccn1-null mice are impaired in cardiac valvuloseptal morphogenesis, resulting in severe atrioventricular septal defects. PMID: 17023674
  30. Constitutively active rhFOXO3a gene transduction suppressed rmCYR61 expression in rat VSMC. Suppression of CYR61 is among several mechanisms by which FOXO3a inhibits VSMC proliferation and neointimal hyperplasia. PMID: 17234971
  31. Intracolonic overexpression of CCN1 induced angiogenesis in mouse colon. PMID: 18599605
  32. Caveolin-1 (cav-1)/Cyr61 interaction via integrins represents a novel pathway of Cyr61 signaling involving cav-1-dependent processes, which play a critical role in regulating hyperoxia-induced cell death PMID: 18801924
  33. Fas-dependent hepatic apoptosis induced by an agonistic monoclonal anti-Fas antibody or intragastric administration of alcohol is severely blunted in knock-in mice expressing an apoptosis-defective Ccn1 allele. PMID: 19364818
  34. Data suggest that signal-controlled activation of SRF, MRTF-A, and CBP provides a novel connection between mechanical stimuli and CCN1 gene expression. PMID: 19542562
  35. The Cyr61 protein, a product of a growth factor-inducible immediate-early gene, regulates chondrogenesis in mouse limb bud mesenchymal cells. PMID: 9441684
  36. Study shows that the interplay between CYR61-CTGF-NOV1 (CCN1) and Wnt5a in endothelial cells and pericytes determines the angiogenic outcome in a model of ischemic retinopathy. Data highlight the significance of CCN1-EC and CCN1-pericyte communication signals in driving physiological and pathological angiogenesis. PMID: 28469167
  37. Mechanical cyclic stretch led to increased Cyr61 mRNA and protein expression, mobilized NF-kappaB from the cytoplasm to the nucleus and increased IL-8 secretion in A549 cells. PMID: 30128877
  38. Study describes how cysteine-rich 61 (CCN1) promotes monocyte migration by upregulating monocyte chemoattractant protein-1 (CCL2) expression in osteoblasts in rheumatoid arthritis (RA) disease. CCN1 could serve as a potential target for RA treatment. PMID: 28341837
  39. Blocking or knockdown CCN1 expression ameliorated skin inflammation and reduced the expression of CCL20 in both imiquimod and IL-23-induced psoriasis-like mouse models. PMID: 28602508
  40. refine the leukocyte recruitment cascade model by introducing endothelium-bound CCN1 as an inflammation mediator and by demonstrating a role for platelets and patrolling Ly6C(low) monocytes in acute vascular inflammation. PMID: 27482114
  41. This study is the first to describe how CCN1 promotes VEGF expression in osteoblasts and increased endothelial progenitor cells angiogenesis in rheumatoid arthritis disease. PMID: 27465842
  42. Data show that cysteine rich protein 61 protein (CCN1) critically mediates doxorubicin (DOX)-induced cardiotoxicity. PMID: 27167338
  43. Our results not only reveal a novel mechanism illustrating the role of Cyr61 in epidermis pathogenesis but also suggest that therapies targeting Cyr61 may represent a novel strategy in the treatment of psoriasis vulgaris. PMID: 27856305
  44. The LPA1-CCN1 axis may be the central control for SMC migration. PMID: 27760754
  45. these data suggest that the WNT/beta-catenin signaling pathway enhances pancreatic cancer development and malignancy in part via up-regulation of CYR61. PMID: 27889647
  46. Lysophosphatidic acid induced time-dependent phosphorylation of serum response factor and CRE-binding protein in in smooth muscle cells, inducing transcription of CCN1. PMID: 28157379
  47. Data show that Cysteine-rich 61 (CYR61) acts as a tumor-promoting gene in pancreatic neuroendocrine tumors. PMID: 26625209
  48. CCN1 is an important regulator of chondrocyte maturation during cartilage development and homeostasis. PMID: 26363286
  49. CCN1 exacerbates tracheal obliterative bronchiolitis by enhancing fibroproliferation via an alphavbeta3-integrin-independent pathway. PMID: 26174800
  50. Data show that RNA silencing of CCN family member 1 protein (CCN1) inhibits retinal neovascularization (RNV) by inhibiting phosphoinositide 3-kinase (PI3K)/AKT protein signaling in mouse pup model of oxygen-induced retinopathy (OIR). PMID: 26459773

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Subcellular Location Secreted
Protein Families CCN family
Tissue Specificity Low in kidney, adrenal gland, testes, brain, and ovary, moderate in heart, uterus, and skeletal muscle, highest in lung.
Database Links

KEGG: mmu:16007

STRING: 10090.ENSMUSP00000029846

UniGene: Mm.1231

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