Mouse Protein Wnt-5a(WNT5A) ELISA kit

Code CSB-EL026138MO
Size 96T,5×96T,10×96T
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Product Details

Target Name
wingless-type MMTV integration site family, member 5A
Alternative Names
Wnt5a ELISA Kit; Wnt-5a ELISA Kit; Protein Wnt-5a ELISA Kit
Uniprot No.
Mus musculus (Mouse)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
3.12 pg/mL-200 pg/mL
0.78 pg/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Stem Cells
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of mouse WNT5A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %92
Range %86-98
1:2Average %98
Range %92-106
1:4Average %94
Range %88-98
1:8Average %88
Range %83-94
The recovery of mouse WNT5A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9587-98
EDTA plasma (n=4)9895-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
2002.755 2.652 2.704 2.536
1002.324 2.376 2.350 2.182
501.639 1.752 1.696 1.528
250.954 1.035 0.995 0.827
12.50.565 0.524 0.545 0.377
6.250.372 0.359 0.366 0.198
3.120.257 0.259 0.258 0.090
00.171 0.165 0.168  
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Mouse WNT5A ELISA Kit was designed for the quantitative measurement of Mouse WNT5A protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL .


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Does this Elisa kit CSB-EL026139MO, does this only show reactivity with mouse and not humans?

Thanks for your inquiry! Protein Wnt-6a is protein macromolecule. The protein level AA sequence of different species (human and house) is different, and the structure is also various. Generally it does not make the protein cross-analysis with inter-specie

Target Background

(From Uniprot)
Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes.
Gene References into Functions
  1. Study shows that the interplay between CYR61-CTGF-NOV1 (CCN1) and Wnt5a in endothelial cells and pericytes determines the angiogenic outcome in a model of ischemic retinopathy. Data highlight the significance of CCN1-EC and CCN1-pericyte communication signals in driving physiological and pathological angiogenesis. PMID: 28469167
  2. Activating effect of WNT5A regulated bone formation in response to hindlimb unloading in mice, and pretreatment with WNT5A partly rescued the osteoporosis caused by mechanical unloading. PMID: 30007964
  3. calcineurin is a key regulator of Wnt5a-induced AQP2 activation without affecting intracellular cAMP level and PKA activity. PMID: 27892464
  4. The findings demonstrate that induction of Vangl protein phosphorylation plays an essential role in transducing Wnt5a signaling to establish Planar cell polarity (PCP) in mammalian development, suggesting a phosphorylation-regulated "Vangl activity gradient" model in addition to the well-documented "Fz activity gradient" model in Wnt/PCP signaling. PMID: 29056748
  5. Post-mitotic filopodial "pathfinding" is guided by mesenchymal WNT5A. Without WNT5A, some cells fail to tether basally and undergo apoptosis, leading to a shortened midgut. PMID: 30016620
  6. We demonstrate with genetic evidence that the Wnt5a gradient acts as a global cue that is instructive in establishing planar cell polarity (PCP) in the limb mesenchyme, and that Wnt5a also plays a permissive role to allow Fgf4 and Fgf8 signaling to orient PCP. PMID: 29615464
  7. Butyrate and bioactive proteolytic form of Wnt-5a regulate colonic epithelial proliferation and spatial development. PMID: 27561676
  8. These data suggest that Wnt5a modulates the development of arthritis by promoting inflammation and osteoclast fusion, and provide the first mouse genetic evidence of a role for endogenous Wnt5a in autoimmune disease. PMID: 28724439
  9. Here, we show that deletion of a single Wnt family member, Wnt5a, is sufficient to elicit profound disruptions in synaptic plasticity, structural maintenance, and learning and memory in adult mice, identifying the importance of this particular noncanonical Wnt in later-life functions. PMID: 28069946
  10. Wnt5a promotes IL-6 and TIMP-1 release from mouse cardiac fibroblasts. PMID: 28357477
  11. bivalent histone codes during odontogenic differentiation PMID: 28880717
  12. WNT5A promoted spermatogonial stem cells activity both in vitro and in vivo. PMID: 27509137
  13. results indicate that Wnt5a signaling restricts infection by antimony drug-sensitive and -resistant Leishmania donovani strains, at least in part by prohibiting parasite niche formation within host macrophages PMID: 28659356
  14. During mouse embryonic stem cell (ESC) in vitro osteogenesis, the noncanonical WNT5a is expressed early on. PMID: 26956615
  15. This study shows that Wnt5a is not required for development of the renal medulla and that loss of the renal medullary region in the Wnt5a-deleted kidney is caused by an abnormal ureter-bladder connection. PMID: 27923152
  16. WNT5A in the bone marrow niche is required to regenerate hematopoietic stem cells and leukemic cells with functional ability to rearrange the actin cytoskeleton and engraft successfully. PMID: 27998927
  17. This study describes the localization and functional role of WNT-5A in human and mouse fibrotic livers. Hepatic WNT-5A expression parallels collagen type I expression. In vivo and in vitro, the myofibroblasts were identified as the key hepatic cells producing WNT-5A. WNT-5A is under control of TGF-beta and its activities are primarily profibrotic. PMID: 28057611
  18. we confirmed the requirement of Wnt5a in the deferoxamine-mediated osteoblast-promoting effects by analyzing the matrix mineralization of Wnt5a-deficient cells. The promoting effect of deferoxamine on matrix mineralization in wild-type cells was completely abolished in Wnt5a(-/-) cells. PMID: 27540134
  19. opposing gradients of Wnt5a and Wnt5b and of their Sfrp inhibitors, together with intercellular signaling via planar cell polarity proteins, polarize node cells along the anterior-posterior axis for breaking of left-right symmetry. PMID: 28292423
  20. Wnt5a is responsible for maintaining effective communication between the stromal and epithelial compartments partly through the action of luminal and stromal secreted factors, Fgf10 and Ihh. PMID: 27965056
  21. findings reveal that FZD9 and heterotrimeric G proteins regulate Wnt-5a signaling and dendritic spines in cultured hippocampal neurons. PMID: 27402827
  22. Here we show that an evolutionarily conserved genomic region named AS3_9 comprises three TEs (AmnSINE1, X6b_DNA and MER117), inserted side-by-side, and functions as a distal enhancer for wnt5a expression during morphogenesis of the mammalian secondary palate. PMID: 27741242
  23. we here reported that Wnt5a mediated activation of canonical Wnt signaling pathway might contribute to the orthodontic force induced bone remodeling. PMID: 27456852
  24. Wnt5a deficiency can regulate inflammatory cytokine secretion, polarization, and apoptosis in MTB-infected macrophage PMID: 27828711
  25. These results showed that Wnt5a could promote the maturation of mast cells via the canonical Wnt signaling pathway and provide important insights into the molecular mechanisms underlying the differentiation of mast cells PMID: 27843938
  26. the CaMKK-CaMKIalpha cascade is required for the axonal growth effect of Wnt5a during neuronal polarization. PMID: 26772170
  27. indicate that restricted expression of Wnt5a in the caudal SpM is essential for normal OFT morphogenesis, and uncover a novel function of spatially regulated cell cohesion by Wnt5a in driving the deployment of SHF cells from the SpM into the OFT PMID: 26916252
  28. Findings indicate wingless-related MMTV integration site 5A protein (WNT5a) as a key regulator of follicle development and gonadotropin responsiveness. PMID: 26667040
  29. The results in a potent increase in odontoblastic cell differentiation, indicating the unique involvement of Atg5, autophagy and Wnt5 signaling in CS/BMP-4-induced differentiation of ES cells into odontoblast-like cells, at a relatively early stage. PMID: 26806855
  30. the Wnt5a/Frizzled-2 axis suppresses beta-catenin signaling in hepatocytes in an autocrine manner, thereby contributing to timely conclusion of the liver regeneration process PMID: 26100214
  31. effects on hypoxic pulmonary hypertension by regulating pulmonary vascular remodeling and right ventricular hypertrophy via reduction in beta-catenin/cyclin D1 signaling PMID: 25956683
  32. Wnt5a regulates the pathogenesis of gp120-induced pain. PMID: 25840108
  33. Study identified a novel Wnt5a/Fzd4 signaling pathway that contributes to the regulation of dendrite morphogenesis PMID: 25424568
  34. The Wnt5a-Ror2 axis promotes the signaling circuit between interleukin-12 and interferon-gamma in colitis PMID: 26030277
  35. study presents the finding that activation of the NF-kappaB pathway by Porphyromonas endodontalis LPS is important for Wnt5a expression in osteoblasts, and that TRIB3 may modulate the sustained expression of Wnt5a in osteoblasts stimulated by P. endodontalis PMID: 25601649
  36. Wnt5a and its receptors are sorted to their correct destination by different mechanisms and that the basolateral secretion of Wnt5a is necessary for apical lumen formation in the epithelial cyst. PMID: 25593127
  37. In Wnt5a null mice, second heart field (SHF) progenitors are trapped in the SHF and fail to be deployed to the outflow tract (OFT) efficiently, resulting in a reduction in the inferior OFT myocardial wall and subpulmonary myocardium. PMID: 25410658
  38. Wnt5a abrogated the inhibitory effects of Wnt16 on Rankl-induced osteoclastogenesis PMID: 26093292
  39. Therefore, Wnt5a-induced noncanonical signaling cooperates with Wnt/beta-catenin signaling to achieve proper bone formation. PMID: 24670389
  40. findings demonstrate, for the first time, that Wnt5a reduces the accumulation of cholesterol in lipid-loaded cells by regulating the mRNA expression of Caveolin-1 and ABCA1, which are involved in reverse cholesterol transport. PMID: 24827906
  41. There is an important role of Wnt5a in kidney development. Disrupted Wnt5a results in kidney cysts in zebrafish and pleiotropic abnormal kidney development in mice. PMID: 25412793
  42. Skin of Wnt5a TG mice was not psoriatic, but characterized with normal proliferation and homeostasis of epidermis. Instead, the TG mice displayed impaired hair follicle transition from telogen to anagen, likely due to impaired canonical Wnt signalling. PMID: 25219536
  43. Non-canonical Wnt5a/Ror2 signaling regulates kidney morphogenesis by controlling intermediate mesoderm extension. PMID: 25082826
  44. Adenovirus-mediated Wnt5a silencing in vivo attenuates the development of atherosclerotic disease. PMID: 25050997
  45. Wnt5a through Wnt/JNK signaling alone or both Wnt/JNK and Wnt/PKC signaling promoted the differentiation of mMSCs into type II alveolar epithelial cells and the migration of mMSCs. PMID: 24658098
  46. Wnt5a regulates HSC quiescence and hematopoietic repopulation through the Ryk receptor and that this process is mediated by suppression of reactive oxygen species. PMID: 23939973
  47. The activation of Wnt5a-Ror2 signaling in epithelial cells undergoing epithelial-to-mesenchymal transition (EMT) may play an important role in disrupting TBM via MMP-2 induction during renal fibrosis. PMID: 23755735
  48. The results indicate an important role of Wnt5a-Ror2 signaling in morphogenesis of the metanephric mesenchyme to ensure proper epithelial tubular formation of the ureteric bud required for kidney development. PMID: 24891614
  49. Wnt5a-mediated Ryk activation triggers divergent responses in callosal axons and dendrites in the in vitro context. PMID: 24471468
  50. Studied expression of Wnt family member 5A in murine hair cycle and its inhibitory effects on follicle in vivo. PMID: 24507677

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Subcellular Location
Secreted, extracellular space, extracellular matrix. Secreted.
Protein Families
Wnt family
Tissue Specificity
Expressed in a gradient at the caudal end of the embryo during gastrulation and later in the distal-most aspect of several structures that extend from the body such as the limbs and genital tubercle.
Database Links
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