Mouse Protein Wnt-5a(WNT5A) ELISA kit

Code CSB-EL026138MO
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P22725
Lead Time 5-7 working days
Abbreviation WNT5A
Protein Biological Process 1 Developmental Protein
Target Name wingless-type MMTV integration site family, member 5A
Alias hWNT5A, WNT-5A protein
Species Mus musculus (Mouse)
Protein Biological Process 3 Chondrogenesis
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Stem Cells
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse WNT5A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %92
Range %86-98
1:2Average %98
Range %92-106
1:4Average %94
Range %88-98
1:8Average %88
Range %83-94
Recovery
The recovery of mouse WNT5A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9587-98
EDTA plasma (n=4)9895-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
2002.755 2.652 2.704 2.536
1002.324 2.376 2.350 2.182
501.639 1.752 1.696 1.528
250.954 1.035 0.995 0.827
12.50.565 0.524 0.545 0.377
6.250.372 0.359 0.366 0.198
3.120.257 0.259 0.258 0.090
00.171 0.165 0.168  
References
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Function Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes.
Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families Wnt family
Tissue Specificity Expressed in a gradient at the caudal end of the embryo during gastrulation and later in the distal-most aspect of several structures that extend from the body such as the limbs and genital tubercle.
Database Links

KEGG: mmu:22418

STRING: 10090.ENSMUSP00000064878

UniGene: Mm.287544

Pathway Hippo signaling pathway
Melanogenesis
mTOR signaling pathway
Wnt signaling pathway
Signaling pathways regulating pluripotency of stem cells

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