Mouse Proto-oncogene Wnt-3(WNT3) ELISA kit

Code CSB-EL026135MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Mouse WNT3 ELISA Kit was designed for the quantitative measurement of Mouse WNT3 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 39.07 pg/mL-2500 pg/mL and the sensitivity is 9.77 pg/mL.

Target Name wingless-type MMTV integration site family, member 3
Alternative Names Wnt3 ELISA Kit; Int-4 ELISA Kit; Wnt-3 ELISA Kit; Proto-oncogene Wnt-3 ELISA Kit; Proto-oncogene Int-4 ELISA Kit
Abbreviation WNT3
Uniprot No. P17553
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 39.07 pg/mL-2500 pg/mL
Sensitivity 9.77 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Stem Cells
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse WNT3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 86    
Range % 82-91    
1:2 Average % 101    
Range % 98-105    
1:4 Average % 87    
Range % 84-91    
1:8 Average % 100    
Range % 95-105    
Recovery
The recovery of mouse WNT3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 89 85-94    
EDTA plasma (n=4) 107 103-110    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
2500 2.547 2.601 2.574 2.411    
1250 2.291 2.200 2.246 2.083    
625 1.543 1.601 1.572 1.409    
312.5 0.971 0.968 0.970 0.807    
156.25 0.572 0.601 0.587 0.424    
78.13 0.414 0.400 0.407 0.244    
39.07 0.295 0.290 0.293 0.130    
0 0.161 0.164 0.163      
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Required for normal gastrulation, formation of the primitive streak, and for the formation of the mesoderm during early embryogenesis. Required for normal formation of the apical ectodermal ridge and for normal embryonic limb development.
Gene References into Functions
  1. Downregulation of Gata4 could restore the phenotype exhibited by Wnt3 downregulation in dorsal root ganglion neurons. PMID: 29567480
  2. Here, we describe the culture protocol using Wnt-3a for successful maintenance of skin epithelial stem cells . PMID: 27032938
  3. stem-cell membranes constitute a reservoir for Wnt proteins, while Frizzled receptor turnover and 'plasma membrane dilution' through cell division shape the epithelial Wnt3 gradient PMID: 26863187
  4. timed secretion of wnt3 by ingrowing axons from the thalamus regulates the combinatorial composition of ribosomal proteins in developing neocortex. WNT3 further regulates the specificity of mRNA translation and development of neurons and oligodendrocytes. PMID: 26245956
  5. this article shows that absence of Wnt3 in the posterior visceral endoderm leads to delayed formation of the primitive streak and that interplay between anterior and posterior visceral endoderm restricts the position of the primitive streak PMID: 25907228
  6. Data indicate that functional Wnt3/beta-Catenin signaling is localized to the dorsal subventricular zone (SVZ) microdomain. PMID: 24449255
  7. Wnt3 is part of a signaling loop that affects homeostasis of intestinal stem and Paneth cells in mice. PMID: 22922422
  8. Wnt3 function in the epiblast is required for the maintenance but not the initiation of gastrulation in mice PMID: 23085236
  9. These results suggest that the regulation of paracrine factors plays a critical role in hippocampal aging and neurogenesis. PMID: 21746862
  10. Wnt-3a and Wnt-3 factors signal through the canonical Wnt/beta-catenin pathway to regulate different aspects of spinal cord neural progenitor development. PMID: 20722074
  11. The mRNA levels of Wnt3, 6, and 9a were significantly upregulated in the intestinal epithelial cells by Salmonella. PMID: 20083111
  12. Different permissive Wnt3/beta-catenin signaling thresholds is required for the embryonic development of head structures and adult intestinal polyposis. PMID: 20084116
  13. WNT-3, expressed by motor neurons, acts as a retrograde signal that controls terminal arborization of muscle afferents. PMID: 12354395
  14. Expression is regulated by beta-catenin during formation of the anteroposterior axis and the mesoderm PMID: 14623818
  15. effects of Wnt3a are exerted through activation of the beta-catenin signal transduction pathway and do not require alternative Wnt transduction cascades PMID: 15009701
  16. Data suggest that recruitment of Axin and Frat1 to the membrane by LRP5-triggered Wnt3 signaling leads to both Axin degradation and Frat1-mediated inhibition of glycogen synthase kinase-3. PMID: 15699046
  17. Wnt3a and SB216763 promoted the proliferation of retinal stem cells, partly dependent on FGF2 signaling.A combination of Wnt and FGF signaling may provide a therapeutic strategy for in vitro expansion or in vivo activation of adult retinal stem cells. PMID: 16223856
  18. Wnt3 repulsion is mediated by Ryk, expressed in a ventral-to-dorsal decreasing gradient PMID: 16280981
  19. We further delineate the role of Wnt signaling pathway as evidenced by induction of Wnt3 and Wnt8b, stimulation of FRP-1, inhibition of GSK-B, accumulation of cytosolic beta-catenin, and transcription of target genes, including c-myc and cyclin-D1. PMID: 16522160
  20. This study findings reveal the rich contributions of the Wnt3a lineage to a variety of brain structures and show that functionally integrated nuclei can share a molecular identity. PMID: 17701978
  21. Wnt3 expression in the epiblast is required for induction of the primitive streak and mesoderm whereas activity in the posterior visceral endoderm is dispensable. PMID: 18028899
  22. an internally truncated LRP5 receptor is strongly implicated in deregulated activation of the WNT/beta-catenin signaling pathway in hyperparathyroid tumors PMID: 18044981
  23. head development is sensitive to the level of WNT3 signalling and that DKK1 is the key antagonist that modulates WNT3 activity during anterior morphogenesis. PMID: 18403408
  24. Wnt-3a signaling regulates hemopoiesis through stromal cells with selectivity and different degrees of the effect, depending on the hemopoietic lineage in the hemopoietic microenvironment. PMID: 11441081

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Subcellular Location Secreted, extracellular space, extracellular matrix. Secreted.
Protein Families Wnt family
Tissue Specificity Detected at low levels in adult brain. Dorsal portion of the neural tube, dorsal ectoderm, the branchial arches, and the limb buds.
Database Links

KEGG: mmu:22415

STRING: 10090.ENSMUSP00000000127

UniGene: Mm.159091

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