Mouse monocyte chemotactic protein 2,MCP-2 ELISA kit

Code CSB-E07428m
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Mouse CCL8 ELISA Kit was designed for the quantitative measurement of Mouse CCL8 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.

Target Name chemokine (C-C motif) ligand 8
Alternative Names Ccl8 ELISA Kit; Mcp2 ELISA Kit; Scya8C-C motif chemokine 8 ELISA Kit; Monocyte chemoattractant protein 2 ELISA Kit; Monocyte chemotactic protein 2 ELISA Kit; MCP-2 ELISA Kit; Small-inducible cytokine A8 ELISA Kit
Abbreviation CCL8
Uniprot No. Q9Z121
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 6.25 pg/mL-400 pg/mL
Sensitivity 1.56 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse MCP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %87
Range %85-88
1:2Average %102
Range %98-106
1:4Average %98
Range %96-101
1:8Average %92
Range %89-95
Recovery
The recovery of mouse MCP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8884-92
EDTA plasma (n=4)9592-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
4002.955 2.781 2.868 2.720
2002.138 2.156 2.147 1.999
1001.445 1.532 1.489 1.341
500.921 0.903 0.912 0.764
250.584 0.561 0.573 0.425
12.50.333 0.324 0.329 0.181
6.250.241 0.253 0.247 0.099
00.142 0.153 0.148
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Chemotactic factor that attracts monocytes. This protein can bind heparin.
Gene References into Functions
  1. Findings exemplify how gradients of chemoattractive factors such as CCL8, drive metastasis and suggest that interference with their operation may provide means for breast cancer management. PMID: 27181207
  2. these data reveal an important role for BLIMP1 in modulating host defenses by suppressing expression of the chemokine CCL8. PMID: 24477914
  3. Results indicate that the induction of MCP-2/CCL8 by mycobacteria is dependent on the activation of TLR2/PI3K/Akt signaling pathway. PMID: 23418602
  4. Vitamin B6 supplementation downregulates Cd8a and Ccl8 mRNA expression, so these candidate genes may play a protective role against colonic aberrant crypt foci development. PMID: 22495988
  5. early and preclinical expression of CCL8 in plasma predicts overall survival of graft-vs.-host disease (GVHD) mice. Together with an involvement of allo-recognition in CCL8 expression, it suggests that CCL8 plays an important role in GVHD pathology. PMID: 21782767
  6. MCP/CCL8 was induced via STAT3 and exhibited anti-tumor metastatic effect PMID: 21624767
  7. promotes atopic dermatitis by recruiting IL-5+ Th2 cells PMID: 21217759
  8. CCL8 proteins were present in the vast majority of tissues investigated. mRNA for these proteins was also expressed in most of these tissues suggesting local production and the ability to respond in situ to inflammatory stimuli. PMID: 20931267
  9. Data show that that the stromal cell-derived chemokines CXCL12 and CCL8 cooperate to attract hematopoietic progenitors with the potential to differentiate into regulatory DCs. PMID: 20624948
  10. CCL8 is a promising specific serum marker for the early and accurate diagnosis of graft-versus-host disease. PMID: 18256320
  11. An elevated plasma CCL8 concentration may be a promising plasma marker for GVHD in mouse models PMID: 19302923

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Subcellular Location Secreted.
Protein Families Intercrine beta (chemokine CC) family
Database Links

KEGG: mmu:20307

STRING: 10090.ENSMUSP00000009329

UniGene: Mm.42029

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