Mouse monocyte chemotactic protein 3,MCP-3 ELISA kit

Code CSB-E07426m
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
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Product Details

Target Name
chemokine (C-C motif) ligand 7
Alternative Names
Ccl7 ELISA kit; Fic ELISA kit; Mcp3 ELISA kit; Scya7C-C motif chemokine 7 ELISA kit; Intercrine/chemokine MARC ELISA kit; Monocyte chemoattractant protein 3 ELISA kit; Monocyte chemotactic protein 3 ELISA kit; MCP-3 ELISA kit; Protein FIC ELISA kit; Small-inducible cytokine A7 ELISA kit
Abbreviation
CCL7
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
1.56 pg/mL-100 pg/mL
Sensitivity
0.39 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse MCP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 86-99  
1:2 Average % 97  
Range % 90-101  
1:4 Average % 93  
Range % 85-96  
1:8 Average % 97  
Range % 92-101  
Recovery
The recovery of mouse MCP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 88 84-92  
EDTA plasma (n=4) 100 91-104  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
100 2.610 2.500 2.555 2.448  
50 2.197 2.125 2.161 2.054  
25 1.451 1.466 1.459 1.352  
12.5 0.750 0.776 0.763 0.656  
6.25 0.346 0.340 0.343 0.236  
3.12 0.240 0.233 0.237 0.130  
1.56 0.168 0.159 0.164 0.057  
0 0.106 0.108 0.107    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

CUSABIO's mouse MCP-3 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MCP-3 in serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of MCP-3 in samples. The color intensity is measured at 450 nm via a microplate reader.

MCP-3, also called CCL7, is a chemotactic factor and attractant for various kinds of leukocytes, including monocytes and neutrophils. CCL7 is involved in anti-inflammatory responses through binding to its chemokine receptors CCR1, CCR2, CCR3, and CCR5 to mediate the recruitment of immune cells. Abnormal CCL7 expression is related to certain immune diseases. Furthermore, CCL7 plays a pivotal role in tumorigenesis. CCL7 promotes tumor progression by supporting the formation of the tumor microenvironment (TME) and facilitating tumor invasion and metastasis.

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Target Background

Function
(From Uniprot)
Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity.
Gene References into Functions
  1. The suppression of nasal inflammation due of IL-17A deficiency in allergic rhinitis is partly responsible for the regulation of CCL7 secretion and eosinophil infiltration, which may be regulated via the CCL7/CCR3 pathway. PMID: 28046055
  2. Our results demonstrate that CCL7 is required for maximal ovalbumin-induced ocular anaphylaxis, mast cell recruitment in vivo, and maximal FcepsilonRI-mediated mast cell activation in vitro PMID: 27956527
  3. Periprostatic adipocytes drive prostate cancer progression in obesity via CCL7 secretion which stimulates CCR3 expressing tumor cells. PMID: 26756352
  4. TFPI has an anti-proliferative role in TNF-alpha stimulated-VSMCs at least partly by interfering with the MCP-3/CCR2 pathway and then via suppression of the ERK1/2 and PI3K/AKT signaling pathways PMID: 26302185
  5. Our data highlight a pivotal role of CCL7 and IRF-7 in rhinovirus-induced inflammation PMID: 25847975
  6. CCL7 has a dual role in the development of renal tubular interstitial fibrosis, deleterious in early stages but beneficial during later stages PMID: 23872063
  7. These findings suggest that aortic MCP-3 overexpression may contribute to the development of atherosclerosis and hepatic steatosis under atherogenic conditions. PMID: 23462015
  8. An increase in the expression of MCP-3 along with IL-6 histone modification at the MCP-3 promoter promotes pain sensation associated with enhanced interaction between astrocytes and microglia in the spinal cord PMID: 23364351
  9. Electrical stimulation for 1 h significantly upregulates SDF-1 and MCP-3 expression that persists for 24 hours. PMID: 22006493
  10. Direct anal sphincter injury results in higher levels of SDF-1 and MCP-3 expression soon after injury, whereas denervation via pudendal nerve crush results in greater SDF-1 and MCP-3 expression 10 days after injury. PMID: 21706136
  11. Matrix metalloproteinase-2-mediated chemokine cleavage of MCP3 has an important role in cardiac inflammation as a negative feedback mechanism. PMID: 21986287
  12. expression of the chemokine, stromal cell-derived factor-1 (SDF-1) or monocyte chemotactic protein-3 (MCP-3) may enhance homing of osteogenic cells into sites of fracture repair PMID: 21567452
  13. CCL7 proteins were present in the vast majority of tissues investigated. mRNA for these proteins was also expressed in most of these tissues suggesting local production and the ability to respond in situ to inflammatory stimuli. PMID: 20931267
  14. MCP-3 is significantly over-expressed in the urethral tissues of both wild-type and obese mice immediately after any urethral manipulation. Underlying obesity resulted in alterations in response to tissue injury, paralleling the degree of injury. PMID: 20970834
  15. Monocyte chemoattractant protein-3 plays a critical role in mediating oxidative stress-induced neutrophilic airway inflammation and may have relevance in induction of neutrophilia in severe asthma. PMID: 11777981
  16. in study of relevance of chemokine expression to selective migration of t-cells and the disease localization in murine graft-versus-host disease, mcp3 was found to be predominantly expressed in skin and heart, and not spleen and liver. PMID: 12098066
  17. overexpression of MCP-3 in tight-skin mouse skin suggests a novel role for this protein as a fibrotic mediator activating extracellular matrix gene expression in addition to promoting leukocyte trafficking. PMID: 12847692
  18. These results indicate that CCR7-mediated cortex-to-medulla migration of thymocytes is essential for establishing central tolerance rather than for supporting the maturation or export of thymocytes. PMID: 16473829
  19. a novel mechanism for the recruitment of CCR10-positive T cells to skin-draining LN following the rapid release of preformed CCL27 from the epidermis. PMID: 18453562
  20. Cross-talk between MCP-3 and TGFbeta may be critical in the development of fibrosis. PMID: 19038247
  21. Up-regulated MCP-3 production does not compensate for loss of MCP-1; MCP-3 appears to be a less effective mediator of monocyte recruitment than MCP-1. PMID: 19641140

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Subcellular Location
Secreted.
Protein Families
Intercrine beta (chemokine CC) family
Database Links
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