Rat Bone morphogenetic protein 2,BMP-2 ELISA Kit

Code CSB-E04508r
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P49001
Lead Time 3-5 working days
Abbreviation BMP2
Protein Biological Process 1 Developmental Protein
Target Name bone morphogenetic protein 2
Alias BMP2A,
Species Rattus norvegicus (Rat)
Protein Biological Process 3 Chondrogenesis
Sample Types serum, plasma, tissue homogenates
Detection Range 7.8 pg/mL-500 pg/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein belongs to the transforming growth factor-beta (TGFB) superfamily. The encoded protein acts as a disulfide-linked homodimer and induces bone and cartilage formation.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat BMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %95
Range %95-110
1:2Average %84
Range %80-88
1:4Average %100
Range %95-106
1:8Average %98
Range %93-104
Recovery
The recovery of rat BMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8984-93
EDTA plasma (n=4)9388-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1OD2AverageCorrected
5002.714 2.596 2.655 2.534
2502.212 2.142 2.177 2.056
1251.455 1.524 1.490 1.369
62.50.800 0.789 0.795 0.674
31.20.448 0.421 0.435 0.314
15.60.253 0.243 0.248 0.127
7.80.172 0.185 0.179 0.058
00.123 0.118 0.121
References
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Function Induces cartilage and bone formation. Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A-ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4.
Subcellular Location Secreted
Protein Families TGF-beta family
Tissue Specificity Expressed in femur, calvaria, trachea, lung and ovary.
Database Links

KEGG: rno:29373

STRING: 10116.ENSRNOP00000028904

UniGene: Rn.90931

Pathway Hippo signaling pathway
TGF-beta signaling pathway

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