24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of Rat Kim-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
CUSABIO's rat KIM-1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative determination of KIM-1 concentrations in serum, plasma, and urine. This assay exclusively recognizes rat KIM-1. The quantitative sandwich ELISA technique of this kit is based on KIM-1 antibody-KIM-1 antigen interactions and an HRP colorimetric detection system to detect the levels of KIM-1 in samples. The intensity of the color is positively proportional to the amount of bound KIM-1 in the initial step.
KIM-1 is a phosphatidylserine receptor that mediates the phagocytosis of apoptotic bodies and oxidized lipids. It is involved in HAV infections, autoimmunity, immune tolerance, and atopic diseases. In normal conditions, KIM-1 is expressed at low levels in the kidney and other organs, but its expression is remarkably increased following renal injury, especially after ischemia-reperfusion injury, enabling them to engulf apoptotic and necrotic cells during acute kidney injury (AKI). The urinary KIM-1 level is tightly linked to its tissue level and correspondingly related to kidney tissue damage. KIM-1 is a possible long-term renal outcome predictor in addition to being an early biomarker of AKI.
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