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| Code | CSB-E08808r |
| Size | 96T,5×96T,10×96T How to order? |
| Trial Size |  |
| Have Questions? | Leave a Message or Start an on-line Chat |
| Description |
CUSABIO's rat KIM-1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative determination of KIM-1 concentrations in serum, plasma, and urine. This assay exclusively recognizes rat KIM-1. The quantitative sandwich ELISA technique of this kit is based on KIM-1 antibody-KIM-1 antigen interactions and an HRP colorimetric detection system to detect the levels of KIM-1 in samples. The intensity of the color is positively proportional to the amount of bound KIM-1 in the initial step. KIM-1 is a phosphatidylserine receptor that mediates the phagocytosis of apoptotic bodies and oxidized lipids. It is involved in HAV infections, autoimmunity, immune tolerance, and atopic diseases. In normal conditions, KIM-1 is expressed at low levels in the kidney and other organs, but its expression is remarkably increased following renal injury, especially after ischemia-reperfusion injury, enabling them to engulf apoptotic and necrotic cells during acute kidney injury (AKI). The urinary KIM-1 level is tightly linked to its tissue level and correspondingly related to kidney tissue damage. KIM-1 is a possible long-term renal outcome predictor in addition to being an early biomarker of AKI. |
| Target Name | hepatitis A virus cellular receptor 1 |
| Alternative Names | Havcr1; Kim1; Hepatitis A virus cellular receptor 1 homolog; HAVcr-1; Kidney injury molecule 1; KIM-1; T cell immunoglobulin and mucin domain-containing protein 1; TIMD-1; CD antigen CD365 |
| Abbreviation | Kim-1 |
| Uniprot No. | O54947 |
| Species | Rattus norvegicus (Rat) |
| Sample Types | serum, plasma, urine |
| Detection Range | 0.313 ng/mL-20 ng/mL |
| Sensitivity | 0.228 ng/mL |
| Assay Time | 1-5h |
| Sample Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | Intra-assay Precision (Precision within an assay): CV%<8%
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| Linearity | To assess the linearity of the assay, samples were spiked with high concentrations of Rat Kim-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
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| Typical Data | These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
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| ELISA Data Analysis | Watch ELISA data processing video & download Curve Expert if needed |
| Troubleshooting and FAQs |
ELISA kit FAQs |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Lead Time | 3-5 working days |
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| Function (From Uniprot) |
May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4. May play a role in kidney injury and repair.
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| Gene References into Functions |
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| Subcellular Location | Membrane; Single-pass type I membrane protein. |
| Protein Families | Immunoglobulin superfamily, TIM family |
| Tissue Specificity | Expressed at a low level in normal kidney but are increased dramatically in postischemic kidney. Expressed in proliferating bromodeoxyuridine-positive and dedifferentiated vimentin-positive epithelial cells in regenerating proximal tubules. |
| Database Links |
KEGG: rno:286934 STRING: 10116.ENSRNOP00000009573 UniGene: Rn.11154 |
