Rat Kidney injury molecule 1,Kim-1 ELISA Kit

Instructions
Code CSB-E08808r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name hepatitis A virus cellular receptor 1
Alternative Names Havcr1 ELISA Kit; Kim1 ELISA Kit; Hepatitis A virus cellular receptor 1 homolog ELISA Kit; HAVcr-1 ELISA Kit; Kidney injury molecule 1 ELISA Kit; KIM-1 ELISA Kit; T cell immunoglobulin and mucin domain-containing protein 1 ELISA Kit; TIMD-1 ELISA Kit; CD antigen CD365 ELISA Kit
Abbreviation Kim-1
Uniprot No. O54947
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, urine
Detection Range 0.313 ng/mL-20 ng/mL
Sensitivity 0.228 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of Rat Kim-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Target Data

Function May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4. May play a role in kidney injury and repair (By similarity).
Gene References into Functions
  1. High KIM1 expression is associated with decreased proximal tubular epithelial cell function. PMID: 29045706
  2. urinary KIM1 may be a valuable biomarker for the early diagnosis of obstructive AKI, and the use of a colloidal gold immunochromatographic strip may be a promising method for the rapid detection of urinary KIM1. PMID: 28075469
  3. Results show that in contrast to other known molecules indicating renal injury and/or repair mechanisms, the chronic renal expression of Kim-1 is strictly confined to proximal cysts. PMID: 27231899
  4. blood biomarker that specifically reflects acute and chronic kidney injury PMID: 24904085
  5. Ischemic postconditioning can attenuate the renal ischemia reperfusion injury and downregulate the expression of Kim-1. PMID: 22898836
  6. These studies are the first direct demonstration of regulation of KIM-1 in response to oxalate exposure in renal epithelial cells in vitro and in vivo. PMID: 22984472
  7. The most sensitive, noninvasive biomarkers of HCBD-induced renal toxicity in Hanover Wistar rats were urinary alpha-GST and KIM-1. PMID: 21905055
  8. The Kim1 gene is regulated in an epigenetic fashion under conditions of nephrotoxicity. PMID: 20660082
  9. Results show Kim-1 outperforms SCr, BUN and urinary NAG in multiple rat models of kidney injury. PMID: 20458318
  10. KIM-1 as a sensitive molecular marker of different levels of tubular injury PMID: 20305092
  11. Urinary Kim-1 serve as a noninvasive, rapid, sensitive, reproducible method to detect early kidney injury in pathophysiological studies and in preclinical drug development studies for risk-benefit profiling of pharmaceutical agents. PMID: 16174863
  12. These results implicate involvement of kidney injury molecule 1 (Kim-1) in the pathogenesis of proteinuria-induced renal damage/repair. Urinary Kim-1 levels may serve as a marker of proteinuria-induced renal damage. PMID: 16467126
  13. Kim-1 is associated with development of renin-angiotensin system-mediated renal damage PMID: 16896183
  14. Urinary Kim-1 and kidney Kim-1/Havcr1 expression appear to be sensitive and tissue-specific biomarkers that will improve detection of early acute kidney injury PMID: 17934191
  15. Data demonstrate that injured kidney epithelial cells assumed attributes of endogenous phagocytes, and that KIM-1 was directly responsible for phagocytosis in cultured primary rat tubule epithelial cells and also porcine and canine epithelial cell lines. PMID: 18414680
  16. on longitudinal follow-up during antiproteinuric treatment increased renal Kim-1 expression is reversible in proportion to proteinuria reduction, likely reflecting reversibility of early tubular injury PMID: 19225054
  17. Cadmium-induced increase in Kim-1 expression occurs before the onset of necrosis and at a point where there is only a modest level of apoptosis in the proximal tubule. PMID: 19371613
  18. Kim-1 is a sensitive biomarker of the early stages of Cadmium-induced proximal tubule injury. PMID: 19371616
  19. Dipsticks can provide a sensitive and accurate detection of Havcr1. PMID: 19387469
  20. Kim-1 is a promising novel marker for the early, organ specific and noninvasive detection of brain death-induced donor kidney damage. PMID: 19522876

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Subcellular Location Membrane, Single-pass type I membrane protein
Protein Families Immunoglobulin superfamily, TIM family
Tissue Specificity Expressed at a low level in normal kidney but are increased dramatically in postischemic kidney. Expressed in proliferating bromodeoxyuridine-positive and dedifferentiated vimentin-positive epithelial cells in regenerating proximal tubules.
Database Links

KEGG: rno:286934

STRING: 10116.ENSRNOP00000009573

UniGene: Rn.11154

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