Recombinant Human Fibroblast growth factor 8 (FGF8) (Active)

FGF8 isoform B plays a critical role in embryonic patterning, limb development, and tumor progression, making it a key target in both developmental biology and cancer research. This tag-free recombinant human FGF8 spans residues 23–215, covering the full mature sequence of isoform FGF-8B, and demonstrates an ED50 of 21.87 ng/mL in BALB/c 3T3 cell proliferation assays—confirming robust mitogenic potency suitable for cell proliferation studies, angiogenesis models, and in vivo tumor biology experiments. The E. coli expression system produces a non-glycosylated protein that provides a consistent, defined baseline for receptor-ligand binding assays via SPR or ELISA, as well as for neuronal or osteogenic differentiation protocols where glycosylation-independent signaling is under investigation. Purity exceeding 95% by SDS-PAGE combined with endotoxin levels below 1.0 EU/μg satisfies the criteria typically required for sensitive cell-based assays and antibody characterization workflows.