Product Details

Purity

> 95 % as determined by SDS-PAGE

Endotoxin

Less than 0.1 EU/ug as determined by LAL method.

Activity

Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 2.0 μg/ml, corresponding to a specific activity of > 500 IU/mg.

Target Names

GDF6

Uniprot No.

Q6KF10

Research Area

Signal Transduction

Alternative Names

GDF6; BMP13; GDF16Growth/differentiation factor 6; GDF-6; Bone morphogenetic protein 13; BMP-13; Growth/differentiation factor 16

Species

Homo sapiens (Human)

Source

E.Coli

Expression Region

336-455aa

Complete Sequence

TAFASRHGKR HGKKSRLRCS KKPLHVNFKE LGWDDWIIAP LEYEAYHCEG VCDFPLRSHL EPTNHAIIQT LMNSMDPGST PPSCCVPTKL TPISILYIDA GNNVVYKQYE DMVVESCGCR

Mol. Weight

13.6 kDa

Protein Length

Full Length of Mature Protein

Tag Info

Tag-Free

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 um filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Datasheet & COA

Please contact us to get it.

Description

GDF6 (BMP-13) plays a critical role in joint, tendon, and cartilage development, making it a key tool for investigating chondrogenic and osteogenic differentiation pathways. This tag-free recombinant human GDF6, encompassing the full mature protein (aa 336–455), demonstrates robust biological activity with an ED50 below 2.0 μg/ml in ATDC5 alkaline phosphatase induction assays, corresponding to a specific activity exceeding 500 IU/mg—data that supports use in stem cell differentiation studies, receptor-ligand binding assays via SPR or ELISA, and in vivo cartilage regeneration models. The E. coli expression system produces a non-glycosylated homodimer that enables precise structure-activity analyses without glycan heterogeneity, while purity greater than 95% by SDS-PAGE combined with endotoxin levels below 0.1 EU/μg satisfies the criteria typical for cell-based differentiation assays and sensitive in vivo applications.

Target Background

Function

Growth factor that controls proliferation and cellular differentiation in the retina and bone formation. Plays a key role in regulating apoptosis during retinal development. Establishes dorsal-ventral positional information in the retina and controls the formation of the retinotectal map. Required for normal formation of bones and joints in the limbs, skull, digits and axial skeleton. Plays a key role in establishing boundaries between skeletal elements during development. Regulation of GDF6 expression seems to be a mechanism for evolving species-specific changes in skeletal structures. Seems to positively regulate differentiation of chondrogenic tissue through the growth factor receptors subunits BMPR1A, BMPR1B, BMPR2 and ACVR2A, leading to the activation of SMAD1-SMAD5-SMAD8 complex. The regulation of chondrogenic differentiation is inhibited by NOG. Also involved in the induction of adipogenesis from mesenchymal stem cells. This mechanism acts through the growth factor receptors subunits BMPR1A, BMPR2 and ACVR2A and the activation of SMAD1-SMAD5-SMAD8 complex and MAPK14/p38.

Gene References into Functions

findings indicate that increased BMP signaling owing to a GDF6 gain-of-function mutation is responsible for loss of joint formation and profound functional impairment in patients with Multiple Synostoses Syndrome 4. PMID: 26643732
As fetal age increased, the expression of growth differentiation factor 6 was decreased correspondingly with the progress of ossification in vertebral bodies and restricted to cartilaginous regions. PMID: 26184900
BMP13 has a role in enhancing extracellular matrix accumulation and inducing cell migration in certain intervertebral disc cells PMID: 26134557
GDF6 is overexpressed in Leri's pleonosteosis. PMID: 24442880
There was a possible weak association between the rs6982567 near GDF6 and polypoidal choroidal vasculopathy in this replication study with an independent Han Chinese cohort. PMID: 25416513
Deficiency of gdf6 results in photoreceptor degeneration, so demonstrating a connection between Gdf6 signaling and photoreceptor survival. PMID: 23307924
a critical role of HTRA1 in the regulation of angiogenesis via TGF-beta signaling and identified GDF6 as a novel disease gene for AMD. PMID: 22049084
studies show that even though tenogenic (BMP 12 and BMP 13) and osteogenic (BMP2) BMPs bind the same receptors with high affinity they signal much differently and result in differential activation of osteogenic and tenogenic markers PMID: 21702718
induces ligamentogenic differentiation in mesenchymal progenitors PMID: 20334610
Observational study of gene-disease association. (HuGE Navigator) PMID: 20734064
These data suggest a potential role for BMP-13 (the human homologue to GDF-6) in tendon matrix modeling and/or remodeling. PMID: 19492402
Observational study of gene-disease association. (HuGE Navigator) PMID: 20494911
The spectrum of disorders generated by morpholino inhibition and the more severe defects (microphthalmia and anophthalmia) observed at higher doses illustrate the key role of GDF6 in ocular development. PMID: 17236135
Mutation screening of a large and clinically diverse Klippel-Feil syndrome (KFS) cohort has identified GDF6 missense mutations in both familial and sporadic KFS patients. PMID: 18425797
These data establish the important role of growth differentiation factor 6 in ocular and vertebral development. PMID: 19129173
BMP-13 inhibited osteogenic differentiation of bone marrow multipotent mesenchymal stromal cells, implying that functional mutations or deficiency of BMP-13 may allow excess bone formation PMID: 19240811
Observational study of gene-disease association. (HuGE Navigator) PMID: 20057906
Observational study of gene-disease association. (HuGE Navigator) PMID: 18716610

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Involvement in disease

Klippel-Feil syndrome 1, autosomal dominant (KFS1); Microphthalmia, isolated, 4 (MCOP4); Leber congenital amaurosis 17 (LCA17)

Subcellular Location

Secreted.

Protein Families

TGF-beta family

Database Links

HGNC: 4221

OMIM: 118100

KEGG: hsa:392255

STRING: 9606.ENSP00000287020

UniGene: Hs.492277

Code	CSB-AP005971HU
Abbreviation	Recombinant Human GDF6 protein (Active)
MSDS	MSDS Datasheet
Size	$142
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Growth/differentiation factor 6 (GDF6) (Active)

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