CD146 Monoclonal Antibody

Datasheet
Code CSB-MA013563A0m
See More Details Free Antibody trial simple
Product Type Monoclonal Antibody
Size US$350
Uniprot No. P43121
Image
  • Western Blot
    Positive WB detected in: MCF-7 whole cell lysate
    All lanes: CD146 antibody at 1:1250
    Secondary
    Goat polyclonal to Mouse IgG at 1/50000 dilution
    Predicted band size: 72, 58 kDa
    Observed band size: 120 kDa

  • Western Blot
    Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate
    All lanes: CD146 antibody at 1:500
    Secondary
    Goat polyclonal to Mouse IgG at 1/50000 dilution
    Predicted band size: 72, 58 kDa
    Observed band size: 120 kDa

  • Immunofluorescence staining of A375 cells with CSB-MA013563A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence staining of Hela cells with CSB-MA013563A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence staining of MCF-7 cells with CSB-MA013563A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence staining of THP-1 cells with CSB-MA013563A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Overlay histogram showing MCF-7 cells stained with CSB-MA013563A0m (red line) at 1:400. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol
Immunogen Recombinant Human Cell surface glycoprotein MUC18 protein (50-646AA)
Raised in Mouse
Species Reactivity Human
Tested Applications ELISA, WB, IF, FC; Recommended dilution: WB:1:500-1:2000, IF:1:50-1:200
Relevance Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%, Protein G purified
Isotype IgG2a
Clonality Monoclonal
Alias Cell surface glycoprotein MUC18 (Cell surface glycoprotein P1H12) (Melanoma cell adhesion molecule) (Melanoma-associated antigen A32) (Melanoma-associated antigen MUC18) (S-endo 1 endothelial-associated antigen) (CD antigen CD146), MCAM, MUC18
Immunogen Species Human
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Clone No. 4D10C7
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Function Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
Subcellular Location Membrane, Single-pass type I membrane protein
Tissue Specificity Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progress
Database Links

HGNC: 6934

OMIM: 155735

KEGG: hsa:4162

STRING: 9606.ENSP00000264036

UniGene: Hs.599039

Q&A and Customer Reviews

Customer Reviews
  • Tested application: WB
    Test sample: Human aorta
    Sample volume: 10μg
    Primary antibody dilution ratio: 1:500
    Review: Normal use of antibodies.
  • Tested application: IHC
    Test sample: Breast cancer tissue section
    Primary antibody dilution ratio: 1:100
    Review: CD146 and GAPDH had good effect in this experiment, with uniform staining and obvious effect.

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