Product Details

Purity

Greater than 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Human CD74 at 2 μg/mL can bind Anti-CD74 recombinant antibody (CSB-RA004956A1HU), the EC₅₀ is 1.317-1.646 ng/mL.

Target Names

CD74

Uniprot No.

P04233

Alternative Names

(HLA-DR antigens-associated invariant chain)(Ia antigen-associated invariant chain)(Ii)(CD antigen CD74)

Molecular Characterization

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

73-232aa

Target Protein Sequence

QQQGRLDKLTVTSQNLQLENLRMKLPKPPKPVSKMRMATPLLMQALPMGALPQGPMQNATKYGNMTEDHVMHLLQNADPLKVYPPLKGSFPENLRHLKNTMETIDWKVFESWMHHWLLFEMSRHSLEQKPTDAPPKESLELEDPSSGLGVTKQDLGPVPM

Mol. Weight

21.0 kDa

Protein Length

Partial of Isoform 2

Tag Info

N-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant CD74 protein gene sequence Gln73-Met232 fused with an N-terminal 10xHis-tag was inserted into the prokaryotic expression vector to obtain the recombinant plasmid, which was transferred into mammalian cells for further expression. The final product is the recombinant human CD74 protein. Its purity reaches up to 95%. Its endotoxin and activity have been measured.

CD74 is the receptor for the cytokine MIF. CD74-MIF interaction activates CD74/CD44 complex further inducing the activation of ERK, PI3K-Akt, NF-κB, and AMPK pathways involved in cell proliferation and survival. Additionally, CD74-MIF signaling plays a vital role in protecting against injury and promoting healing in different body parts. CD74 also participates in the modulation of T cell and B cell development, dendritic cell (DC) motility, macrophage inflammation, and thymic selection. Many studies have shown that CD74 is complicated in the pathogenesis of many inflammatory diseases, such as Alzheimer's disease, systemic lupus erythematosus, and type I diabetes. CD74 expression has been proposed as a prognostic factor in numerous malignancies, with higher relative expression acting as a sign of tumor progression.

Target Background

Function

Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.; Binds to the peptide-binding site of MHC class II alpha/beta heterodimers forming an alpha-beta-CLIP complex, thereby preventing the loading of antigenic peptides to the MHC class II complex until its release by HLA-DM in the endosome.; Stabilizes the conformation of mature CTSL by binding to its active site and serving as a chaperone to help maintain a pool of mature enzyme in endocytic compartments and extracellular space of antigen-presenting cells (APCs). Has antiviral activity by stymieing the endosomal entry of Ebola virus and coronaviruses, including SARS-CoV-2. Disrupts cathepsin-mediated Ebola virus glycoprotein processing, which prevents viral fusion and entry. This antiviral activity is specific to p41 isoform.

Gene References into Functions

High CD74 expression is associated with progressive multiple sclerosis. PMID: 28923927
These findings predict the binding mode of Hu-MIF-1 and orthologs with CD74. PMID: 28513076
results suggest that two immune biomarkers, CD74 and IL10, could be relevant tools for the identification of Intensive care unit-acquired infections risk in Intensive care unit patients. PMID: 28477143
Increased CD74 expression levels predicted worse stroke severity and outcomes in subjects with ischemic stroke. PMID: 27884769
The data validate CD74 as a useful prognostic tumor cell protein marker associated with favorable recurrence-free survival and overall survival in stage III melanoma. PMID: 26783288
These results have implications for the manner in which D-DT and MIF compete with each other for binding to the CD74 receptor and for the relative potency of DRa1-MOG-35-55 and RTL1000 for competitive inhibition of D-DT and MIF binding and activation through CD74. PMID: 27573366
CLIP expression and activated gamma-delta T cells are responsible for the development of preeclampsia. PMID: 28642294
these observations are consistent with the view that CD74 expression in tumor cells promotes the intratumoral immune response and is associated with a better prognosis in basal-like breast cancer PMID: 27058619
MIF-CD74 signaling inhibits interferon (IFN)-gamma secretion in microglia through phosphorylation of microglial ERK1/2 (extracellular signal-regulated protein kinases 1 and 2). The inhibition of MIF signaling or its receptor CD74 promotes IFN-gamma release and amplifies tumor death either through pharmacological inhibition or through siRNA-mediated knockdown. PMID: 27157615
High CD74 expression is associated with colorectal cancer. PMID: 27599658
the roles of CD74 and MIF in the immune surveillance escape process PMID: 26866879
CD74 downregulation in placental macrophages is present in preeclampsia. PMID: 27199465
CD74 was increased in burn patients. PMID: 27209369
predicted binding of one CD74 trimer to a single RTL1000 antagonist utilized the same two 5 residue determinants PMID: 26851955
an inverse correlation with the tumor size for stromal MIF and a positive correlation with the triple receptor negative tumor status for stromal CD74 seem to be showed PMID: 26412712
Studies indicate that the invariant chain (CD74) is critical for antigen presentation. PMID: 27033518
FOXP1 represents a novel regulator of genes targeted by the class II MHC transactivator CIITA and CD74. PMID: 26500140
Interferon gamma promotes melanoma cell survival regulating CD74-MIF interaction. PMID: 26039541
The results of this stusy suggested that the CD74 represents a positive prognostic marker most probably because of its association with an M1-polarized immune milieu in high-grade gliomas. PMID: 25175718
Invariant chain is a new chaperone for TLR7 in B cells. (Review) PMID: 26198699
CD74 expression and its therapeutic potential in thyroid carcinoma PMID: 25600560
Specific targeting of the CD74 on the lymphoma cell surface will sensitize CD74-expressing cancer cells to Fas-mediated apoptosis. PMID: 25304249
A conserved WW domain-like motif regulates CD74 antigen-dependent cell-surface transport of the NKG2D ligand ULBP2. PMID: 25983110
these results demonstrate that the subunit stoichiometry of oligomeric Ii/MHCII complexes is influenced by p35. PMID: 24638068
Findings showed an increased extent of MIF expression in cancer cells and in stromal fibroblasts of Breast Cancer tumor, in contrast to a less uniform increase of CD74 expression. PMID: 24939415
In human B cells, SPPL2a is indispensable for turnover of CD74 N-terminal fragment. PMID: 25035924
Results show that CD74-NRG1 gene fusions are activating genomic alterations in invasive mucinous lung adenocarcinomas. PMID: 24469108
In TPA-induced skin inflammation, MIF is released from damaged keratinocytes and then triggers the chemotaxis of CD74(+)CXCR2(+) NKT cells for IFN-gamma production. PMID: 25172501
Data show that clear cell renal cell carcinoma (ccRCC) tissue and malignant cell lines expressed higher levels of CD74 and hypoxia inducible factor 1alpha (HIF-1alpha) than adjacent normal renal tissue and normal cell HK-2. PMID: 23273913
Polymorphisms in CD74 is associated with hematologic toxicity in patients with non-small-cell lung cancer after platinum-based chemotherapy. PMID: 24220096
MIF/CD74 pathway may represent a crucial target for treating disc degeneration since inhibiting the function of MIF with its antagonist ISO-1 can reduce MIF-induced inflammation and exert potent therapeutic effects. PMID: 24569872
demonstrate that CLIP expression on leukemia-associated phenotype (LAP)-positive cells during follow-up is significantly correlated with a shortened relapse-free survival PMID: 24731748
High CD74 expression is associated with head and neck squamous cell carcinomas. PMID: 24663824
High expression of CD74 is an independent prognostic factor for prolonged overall survival in mesothelioma patients PMID: 24594996
These results demonstrate natural antagonist activity of DRalpha1 for macrophage migration inhibitory factor PMID: 24683185
Recombinant vaccinia virus vaccines encoding CD74 may be useful tools to improve CD4 T-cell responses to viral and tumour antigens. PMID: 24205828
when CD74 is overexpressed in human cancer and noncancerous epithelial cells, it interacts and interferes with the function of Scribble PMID: 23730214
Evaluated expression of CD74 in chronic lymphocytic leukemia patients. CD74 expression was significantly higher in CLL group than in controls. There was positive correlation between CD74 and ZAP70 expression. PMID: 23572149
Our data do not replicate prior reports of LN-2 as a sensitive and specific marker for undifferentiated pleomorphic sarcoma PMID: 23000905
CD74 antigen expression is increased in high grade, invasive urothelial bladder cancer. PMID: 22905972
Ii regulates the repertoire of tumor peptides presented by major histocompatibility complex class II+ breast cancer cells. PMID: 22942358
Data show that human Iip35 isoform (CD74 antigen) is expressed in mouse antigen presenting cells. PMID: 22689013
Data indicate that 25 +/- 1.3% of CD74 and 17 +/- 0.3% of HLA-DR are colocalized. PMID: 22889831
a novel regulatory mechanism governing cell migration during intervertebral disc degeneration PMID: 22952837
High Cd74 expression is associated with lymph node metastasis and triple-negative breast cancer. PMID: 22935920
Hepatitis C virus-mediated inhibition of cathepsin S increases invariant-chain expression on hepatocyte surface. PMID: 22761382
Expression of CD74-ROS in noninvasive NSCLC cell lines readily conferred invasive properties that paralleled the acquisition of E-Syt1 phosphorylation. PMID: 22659450
Stat1 and CD74 overexpression is co-dependent and linked to increased invasion and lymph node metastasis in triple-negative breast cancer. PMID: 22178447
Upregulation of MIF, CD74 and TLR4 are associated with increasing clinical stage and provide an opportunity as novel gastric cancer chemoprevention and/or treatment strategy. PMID: 22611320
This is the first study reporting that Ii chain up-regulation occurs on naturally infected antigen presenting cells obtained directly from HIV(+) subjects. PMID: 21945129

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Involvement in disease

A chromosomal aberration involving CD74 is found in a non-small cell lung tumor. Results in the formation of a CD74-ROS1 chimeric protein.

Subcellular Location

Cell membrane; Single-pass type II membrane protein. Endoplasmic reticulum membrane. Golgi apparatus, trans-Golgi network. Endosome. Lysosome. Note=Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane.; [Isoform p41]: Late endosome. Lysosome.

Tissue Specificity

[Isoform p41]: In B cells, represents 10% of total CD74 expression.; [Isoform p33]: In B cells, represents 70% of total CD74 expression.

Database Links

HGNC: 1697

OMIM: 142790

KEGG: hsa:972

STRING: 9606.ENSP00000009530

UniGene: Hs.436568

Code	CSB-MP004956HU1(F2)
Abbreviation	Recombinant Human CD74 protein, partial (Active)
MSDS	MSDS Datasheet
Size	$118
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized Human CD74 at 2 μg/ml can bind Anti-CD74 recombinant antibody (CSB-RA004956A1HU), the EC₅₀ is 1.317-1.646 ng/mL.
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Recombinant Human HLA class II histocompatibility antigen gamma chain (CD74), partial (Active)

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