CD74 is a membrane protein that exists both on the cell surface and within the intracellular endomembrane system. The CD74 molecule consists of an extracellular domain, a transmembrane domain, and a cytoplasmic domain. Inside the cell, CD74 is also present in the endoplasmic reticulum. CD74 is a key molecule on antigen-presenting cells such as antigen-presenting cells and B cells. It interacts with MHC class II molecules, helping to stabilize these MHC class II molecules and facilitating the processing and presentation of antigens. CD74 also plays a regulatory role in the immune system. It can modulate the activation of T cells and the direction of immune responses. Additionally, CD74 is associated with B cell activation and antibody production. It holds significant importance in the research of immune-related diseases, autoimmune diseases, and cancer immunotherapy. In disease research, CD74 is sometimes used as a potential therapeutic target to interfere with immune responses or regulate the activation of immune cells.
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CD74 at 2 μg/ml can bind Anti-CD74 recombinant antibody (CSB-RA004956A1HU), the EC50 is 1.317-1.646 ng/mL.
● CD74 Recombinant Monoclonal Antibody CSB-RA004956A1HU
Validated Data
The Binding Activity of Human CD74 with Anti-CD74 recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CD74 (CSB-MP004956HU1(F2)) at 2 μg/mL can bind Anti-CD74 recombinant antibody, the EC50 is 1.317-1.646 ng/mL.
● CD74 Recombinant Monoclonal Antibody CSB-RA004956A0HU
Validated Data
IHC image of CSB-RA004956A0HU diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-RA004956A0HU diluted at 1:100 and staining in paraffin-embedded human endometrial tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Overlay histogram showing Raji cells stained with CSB-RA004956A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
The following CD74 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
CD74 Antibodies for Mus musculus (Mouse)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA004956LD01MO | Cd74 Antibody, Biotin conjugated |
Mouse | ELISA |
CSB-PA004956LA01MO | Cd74 Antibody |
Mouse | ELISA, WB |
CD74 Antibodies for Rattus norvegicus (Rat)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA004956LA01RA | Cd74 Antibody |
Rat, Mouse | ELISA, WB |
CD74 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA004956DSR1HU | CD74 Antibody |
Human | ELISA, IHC, IP |
CSB-PA005842 | CD74 Antibody |
Human | WB, ELISA |
CSB-RA004956A0HU | CD74 Recombinant Monoclonal Antibody |
Human | ELISA, IHC, FC |
CSB-RA004956A1HU | CD74 Recombinant Monoclonal Antibody |
Human | ELISA |
CD74 Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-YP004956RA CSB-BP004956RA CSB-MP004956RA CSB-EP004956RA-B |
Recombinant Rat H-2 class II histocompatibility antigen gamma chain (Cd74), partial |
Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP004956RA | Recombinant Rat H-2 class II histocompatibility antigen gamma chain (Cd74), partial |
E.coli |
CSB-CF004956RA | Recombinant Rat H-2 class II histocompatibility antigen gamma chain (Cd74) |
in vitro E.coli expression system |
CD74 Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP004956MO CSB-BP004956MO CSB-MP004956MO CSB-EP004956MO-B |
Recombinant Mouse H-2 class II histocompatibility antigen gamma chain (Cd74), partial |
Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP004956MO | Recombinant Mouse H-2 class II histocompatibility antigen gamma chain (Cd74), partial |
E.coli |
CSB-CF004956MO | Recombinant Mouse H-2 class II histocompatibility antigen gamma chain (Cd74) |
in vitro E.coli expression system |
CD74 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-CF004956HU | Recombinant Human HLA class II histocompatibility antigen gamma chain (CD74) |
in vitro E.coli expression system |
CSB-YP004956HU1 CSB-BP004956HU1 CSB-MP004956HU1 CSB-EP004956HU1-B |
Recombinant Human HLA class II histocompatibility antigen gamma chain (CD74), partial |
Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP004956HU1 | Recombinant Human HLA class II histocompatibility antigen gamma chain (CD74), partial |
E.coli |
CSB-MP004956HU1(F2) | Recombinant Human HLA class II histocompatibility antigen gamma chain (CD74), partial (Active) |
Mammalian cell |
CD74 ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-EL004956HU | Human HLA class II histocompatibility antigen gamma chain(CD74) ELISA kit |
serum, plasma, tissue homogenates | 0.225 ng/mL |