Recombinant Human Sonic hedgehog protein(SHH),partial (Active)

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Code CSB-AP000371HU
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Product Details

Purity >98% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine C3H/10T1/2 cells is less than 1 μg/ml, corresponding to a specific activity of >1.0x103 IU/mg.
Target Names SHH
Uniprot No. Q15465
Research Area Cancer
Alternative Names HHG 1; HHG-1; HHG1; HLP 3; HLP3; Holoprosencephaly 3; HPE 3; HPE3; MCOPCB5; shh; SHH_HUMAN; SMMC I; SMMCI; Sonic Hedgehog (Drosophila) homolog; sonic hedgehog homolog (Drosophila); Sonic hedgehog homolog; Sonic hedgehog protein; Sonic hedgehog protein C-product; TPT; TPTPS
Species Homo sapiens (Human)
Source E.coli
Expression Region 22-197aa
Complete Sequence IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG
Mol. Weight 19.8 kDa
Protein Length Partial(22-24:LAC→IVI)
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 µm filtered 20 mM PB, pH 7.4, 150mM NaCl
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Sonic hedgehog protein
Gene References into Functions
  1. The elevated levels of both serum Shh and IL-6 were mainly observed in BC patients who had a significantly higher risk of early recurrence and bone metastasis, and associated with a worse survival for patients with progressive metastatic BC. PMID: 28496132
  2. Disturbed SHH link between KIF7 and C5orf42 contributes to neurodevelopmental features characteristic of C5orf42-related ciliopathies. PMID: 29321670
  3. Histone deacetylase 6 (HDAC6) inhibition enhanced glioma stem cells (GSCs) radiosensitivity via inactivating sonic hedgehog protein (SHH)/glioma-associated oncogene homolog 1 (Gli1) pathway. PMID: 29222038
  4. Blockade of the Shh signaling pathway could reduce cell proliferation and migration only in MDA-MB-231 cells. Hh pathway inhibitor-1 (HPI-1) increased the percentages of late apoptotic cells in MDA-MB-231 cells and early apoptotic cells in T2 cells PMID: 29734730
  5. Structure-guided mutational analysis shows that interaction between ShhN and Ptch1 is steroid-dependent. PMID: 29954986
  6. Protease nexin-1 prevents growth of human B cell lymphoma via inhibition of sonic hedgehog signaling. PMID: 29483508
  7. SHH-related signaling pathway affects antineoplastic drug resistance in cultured glioma cells. PMID: 29313231
  8. SHH is expressed in cilia in the airway epithelial cells.SHH may mediate noncanonical hedgehog signaling through motile cilia to dampen respiratory defenses. PMID: 29358407
  9. High SHH expression is associated with radioresistance in esophageal adenocarcinoma. PMID: 29715275
  10. Identify SMO-dependent Shh signalling as a specific process for the activation of adventitial fibroblasts and the subsequent proliferation of smooth muscle cells and neointima formation. PMID: 29088375
  11. In conclusion, our data suggest that overexpression of the Hedgehog components SHH, GLI2 and FOXA2 might be used as markers of an aggressive hemangioma. PMID: 28370639
  12. These findings showed the upexpression of sonic hedgehog and vascular endothelial growth factor with co-localization in varicocele veins which imply that the reducing hypoxia or using sonic hedgehog antagonists may be helpful for this vascular disease. PMID: 26867642
  13. Shh and Gli1 expression were associated with lymph node metastasis, TNM stage and tumor recurrence, suggesting Shh and Gli1 protein could become the valuable biomarker in evaluating the lymph node metastasis in oral squamous cell carcinoma. PMID: 28886265
  14. Epithelial-mesenchymal transition programs promote basal mammary stem cell and tumor-initiating cell stemness by inducing primary ciliogenesis and Hedgehog signaling. PMID: 29158396
  15. Case Report: medullablastoma with activated SHH expression. PMID: 29517209
  16. NAFLD progression is usually accompanied by activation of the Sonic hedgehog (SHH) pathway leading to fibrous buildup (scar tissue) and inflammation of the liver tissue. For the first time patients with holoprosencephaly, a disease caused by SHH signaling mutations, are shown to have increased liver steatosis independent of obesity. PMID: 28645738
  17. Gpr161 is a critical factor in the basal suppression machinery of Shh signaling, neural tube morphogenesis and closure. (Review) PMID: 27731925
  18. Oncogenic activation of SHH is associated with Rubinstein-Taybi Syndrome and Medulloblastoma. PMID: 29551561
  19. The results showed that Shh and Gli1 were upregulated in prostate cancer tissues and were targeted by a phytogenic neoplastic compound carnosol. PMID: 28886322
  20. Hh signaling activation might reflect aggressive tumoral behavior, since high epithelial GLI2 expression positively correlates with a higher pathological Gleason score. Moreover, higher epithelial GLI3 expression is an independent marker of a more favorable prognosis. PMID: 28877722
  21. GPT2 reduced alpha-ketoglutarate level in cells leading to the inhibition of proline hydroxylase 2 (PHD2) activity involved in the regulation of HIF1alpha stability. Accumulation of HIF1alpha, resulting from GPT2-alpha-ketoglutarate-PHD2 axis, constitutively activates sonic hedgehog (Shh) signaling pathway. PMID: 28839461
  22. Results show SHH proteolysis is under the mechanism of Scube2 which is enriched at the surface of Shh-producing cells by heparan sulfate proteoglycans. PMID: 27199253
  23. influences sweat gland differentiation of stem cells PMID: 27120089
  24. during Hedgehog signaling, ligand binding inhibits Patched by trapping it in an inactive conformation, a mechanism that explains the dramatically reduced activity of oncogenic Patched1 mutants. PMID: 27647915
  25. In an in vitro model of LPS inflammation of the blood-brain barrier, sonic hedgehog signaling was activated by Wip1 overexpression and inhibited by silencing. Wip1 may protect the BBB against LPS damage via SHH signaling. PMID: 29128669
  26. the effect gene of the Shh pathway, gli1, was found to have a reduced level of expression along with a decreased expression of gli2. PMID: 26446020
  27. SHH can promote cell growth and cell osteoblastic/cementoblastic differentiation via BMP pathway PMID: 27289556
  28. Findings suggest that oral squamous cell carcinoma (OSCC)derived sonic hedgehog protein (SHH) stimulates angiogenesis at the tumor invasive front. PMID: 29187450
  29. Expression of SHH and GLI1 may be useful prognostic markers of Merkel cell carcinoma because increased expression was associated with better prognosis. PMID: 28551328
  30. High SHH expression is associated with esophageal squamous cell carcinoma. PMID: 29054489
  31. Studies suggest significance of other signaling aside from hedgehog in the pathogenesis of basal cell carcinoma (BCC) of the skin. PMID: 28574612
  32. Gorlin syndrome-derived induced pluripotent stem cells (iPSCs) expressed lower basal levels than control iPSCs of the genes encoding the Hh ligands Indian Hedgehog (IHH) and Sonic Hedgehog (SHH). PMID: 29088246
  33. SHH activation is associated with Rhabdomyosarcoma. PMID: 28881358
  34. Studies suggest that embryonic signaling pathways, the likes of Notch, Wnt, and Hedgehog and tumor marker Oct-4 offer targets for cascade-specific molecular inhibition as they are fundamental to (cancer and normal) stem cell maintenance and growth. PMID: 27730468
  35. Methylation at K436 and K595 respectively by Set7 increases the stability and DNA binding ability of Gli3, resulting in an enhancement of Shh signaling activation. PMID: 27146893
  36. Altogether, these data suggested that curcumin inhibited the activities of BCSCs through suppressing Shh pathway, which might be an effective chemopreventive agent for bladder cancer intervention. PMID: 28870814
  37. High SHH expression is associated with Small Cell Lung Cancer. PMID: 28870922
  38. Accumulating evidence suggest that cytochrome P450 (CYP26), the primary retinoid-inactivating enzyme, plays a critical role in the integration of two neoplastic molecular programs: the retinoid metabolism and Hedgehog pathways. (Review) PMID: 28754309
  39. CHSY1 overexpression in HCC contributes to the malignant behavior of hepatocellular carcinoma cells via activation of the hedgehog signaling pathway. PMID: 28652022
  40. We found a novel 7q36.3 duplication involving 2 genes (SHH and RBM33) in a patient with complete corpus callosum agenesis (Figure), moderate learning difficulties, and macrocephaly PMID: 28284480
  41. Study showed that SHH expression was significantly high among breast cancer patients with advanced tumor grade, stage, nodal involvement and metastasis and this expression strongly correlated with proliferation marker. PMID: 28739739
  42. This suggests an important cross-talk between SHH and WIP1 pathways that accelerates tumorigenesis and supports WIP1 inhibition as a potential treatment strategy for MB. PMID: 27086929
  43. YB-1 is induced by Shh in CGNPs PMID: 26725322
  44. SHH siRNA synergistically enhanced cytotoxicity induced by itraconazole in MCF-7 cells. PMID: 27810405
  45. Hedgehog pathway activation in T-cell acute lymphoblastic leukemia predicts response to SMO and GLI1 inhibitors. PMID: 27694322
  46. Data suggest that negative feedback mediated by GLI3 (GLI-Kruppel family member) acts to finely tune SHH (sonic hedgehog) signaling. During medulloblastoma (MB) formation, nerve tissue cells appear to express nestin which hyperactivates SHH signaling by abolishing negative feedback by GLI3. Restoration of intrinsic negative feedback by repressing nestin expression represents a promising approach to treat MB. [REVIEW] PMID: 28389227
  47. The study reveals several novel individual and repetitive mutations of SHH gene in Gallbladder Cancer and Cholelithiasis samples that may be used as diagnostic markers for gallbladder carcinogenesis. PMID: 28058596
  48. Data indicate that agedunin induces its anti-metastatic effect through inhibition of sonic hedgehog protein [SHH] signaling. PMID: 26988754
  49. findings suggest that Usp7 is important for MB cell proliferation and metastasis by activating Shh pathway, and is a putative therapeutic target for MBs PMID: 28137592
  50. importance of MAOA for initiating the pre-metastatic niche in stromal cells and promoting PCa metastasis to bone and visceral organs, mediated by activation of paracrine Shh-IL6-RANKL signaling underlying tumor-stromal interactions. PMID: 28292438
  51. Studies indicate that sonic hedgehog protein (Shh) is upregulated specifically in renal tubular epithelium. PMID: 27333788
  52. these results reveal a novel role for E177-Zn(2+) in regulating SHH signaling that may involve critical, cilia basal-body localized changes in cross-linking and/or conformation. PMID: 28263766
  53. first report of the assessment of the frequency of GLI3/SHH/preZRS/ZRS in Chinese polydactyly patients to show any higher possibility of mutations or variants for the 4 genes or sequences in China PMID: 28127823
  54. SHH acted exclusively in a paracrine fashion on pancreatic stellate cells and influenced the growth of Pancreatic Cancer cells only indirectly, ADM could directly impact the growth of both Pancreatic Cancer cells and pancreatic stellate cells . PMID: 27246849
  55. data implicate perturbation of the Shh pathway in at least 37% of individuals with the hypothalamic hamartoma epilepsy syndrome, consistent with the concept of a developmental pathway brain disease PMID: 27453577
  56. These findings suggest that the Hh signaling pathway in breast CSCs may contribute to the poor outcome of patients with breast cancer. PMID: 27779682
  57. The results suggested that, when the rs61730970, rs200798148 and rs146535482 alleles of the Hedgehog gene lacked particular single nucleotide polymorphisms, the patients were associated with a greater risk of Hirschsprung's disease and/or anorectal malformations [HSCR: odds ratio (OR)=1.543, P=0.004; OR=1.494, P=0.007; rs146535482: OR=1.556, P=0.003, respectively. PMID: 27082974
  58. High expression of SHH is associated with glioma chemoresistance. PMID: 27662839
  59. High SHH expression is associated with pancreatic cancer. PMID: 26815504
  60. our data showed that paracrine Hh signaling mediated pro-angiogenic function of HSC and enhanced hepatoma growth. PMID: 27657189
  61. Oleanolic acid markedly suppressed the activation of the STAT3 and SHH signaling pathways and inhibited the expression of the proangiogenic vascular endothelial growth factor A and basic fibroblast growth factor, two important target genes of the aforementioned signaling pathways. PMID: 27108756
  62. This case, together with two others previously described, one presenting with esophageal atresia, the other with congenital esophageal stenosis, confirms the possible association between congenital esophageal malformations and 7q terminal deletion including SHH. PMID: 27614115
  63. sHH signaling pathway is involved in pancreatic cancer pain, and pancreatic stellate cells play an essential role in the process greatly by inducing Nerve Growth Factors. PMID: 26934446
  64. Study demonstrated that SHH is overexpressed in gastric cancer and is associated with poor survival. PMID: 27039174
  65. Shh protein is upregulated in breast cancer. Expression of this protein was statistically correlated with age and malignant stage. PMID: 26727947
  66. The roles of the SHH pathway in the context of limb development.[ review] PMID: 26762695
  67. The serum levels of IHH and SHH were significantly higher in autistic subjects than those of control subjects. The findings support a correlation between SHH, IHH and BDNF in autistic children, suggesting their pathological role in autism PMID: 26691363
  68. MAPK and SHH pathways modulate type 3 deiodinase expression in papillary thyroid carcinoma PMID: 26825960
  69. during human brain development, Shh plays an important role in the specification of cortical progenitors PMID: 25146370
  70. The disruption of a novel limb cis-regulatory element of SHH is associated with autosomal dominant preaxial polydactyly-hypertrichosis in a large French pedigree. PMID: 25782671
  71. Results obtained from in vitro and in vivo studies demonstrate that Shh may function as an inducer of bladder cancer progression. Its expression correlates with advanced clinical stages and poor prognostic bladder cancer. PMID: 25728352
  72. The sonic hedgehog protein, through its receptors, plays a role in embryo development, in idiopathic pulmonary fibrosis, and in small cell lung carcinoma, non-small cell lung carcinoma and malignant pleural mesothelioma. Review. PMID: 25794996
  73. Crocetinic acid inhibited the expression of both Shh and smoothened in pancreatic cancer stem cells, thereby inhibiting pancreatic tumorigenesis PMID: 26317547
  74. hyperactivity of Hh signaling resulted in EGFR-TKI resistance, by EMT introduction and ABCG2 upregulation, and blockade of Hh signaling synergistically increased sensitivity to EGFR-TKIs in primary and secondary resistant NSCLC cells PMID: 26943330
  75. Data suggest that substrate specificity of hedgehog acyltransferase (HHAT) in palmitoylation reaction extends to oligopeptide fragments of Sonic hedgehog protein (SHH). PMID: 26334609
  76. Data indicate that regulation of sonic hedgehog (SHh)-GLI family zinc finger 1 (Gli1) signals to migration of pancreatic cancer AsPC-1 Cells through mediating eukaryotic translation initiation factor 5A (EIF5A2) gene expression. PMID: 26465952
  77. appears to be responsive to the inhibition of GALNT1 and SHH signaling PMID: 26676748
  78. Data suggest a role for hedgehog pathway (HhP) signaling in myeloid disease. PMID: 26483188
  79. High expression of Sonic Hedgehog Protein is associated with Hepatocellular Carcinoma and Intrahepatic Cholangiocarcinoma. PMID: 25740074
  80. PDE4D interacts directly with Neuropilins, positive regulators of Hedgehog signal transduction pathway. PMID: 26371509
  81. Here we show that MITF-A mRNA is predominantly expressed in all three human liver cancer cell lines examined. PMID: 26773496
  82. Study shows that overexpression of SHH and Gli1 correlates with an increased risk along with a poor Myelodysplastic Syndrome prognosis. PMID: 26317501
  83. This article reviews the most relevant functions of Shh during cerebellar ontogenesis, underlying its role in physiological and pathological conditions. [review] PMID: 26499980
  84. Studies indicate crosstalk between Notch receptor and Wnt protein, Hedgehog protein, hypoxia and transforming growth factor beta (TGFbeta)/bone morphogenetic protein (BMP) pathways. PMID: 26592459
  85. Data indicate that resveratrol plus curcumin inhibits Hedgehog-Gli1 components in p21Waf1/Cip1 dependent manner. PMID: 26212257
  86. Na,K-ATPase b1-subunit is a target of the Shh signaling pathway and loss of b1-subunit expression may contribute to tumor development and progression not only in carcinoma but also in medulloblastoma, a tumor of neuronal origin. PMID: 26286140
  87. Data suggest that nicotine increases the SHH-mediated malignant potential of pancreatic cancer stem cells and that GABA prevents these effects. PMID: 26689865
  88. Studies indicate that the hedgehog (Hh) signaling pathway has become one of the most studied potential therapeutic targets in hematological malignancies. PMID: 24991722
  89. It is a regulator in tumorigenesis of hepatocellular carcinoma. PMID: 25682950
  90. Shh and Gli1 are prognostic biomarkers for patients with resected PDAC. PMID: 25552484
  91. The reemergence of SHH in epithelial cells could result in induction of myofibroblast differentiation in a Smo-dependent manner and subsequent Gli1 activation of the alpha-SMA promoter in pulmonary fibrosis. PMID: 25140582
  92. our results demonstrated that upregulation of HIF-1alpha in NB promotes proliferation, migration and invasiveness via SHH signaling. PMID: 25811359
  93. These results highlight the interaction between Wnt and SHH signaling pathways in dying tumor cells. PMID: 25713298
  94. Studied the proteome of human SHH-MB cancer stem-like cells before and after Retinoic Acid (RA)-induced differentiation. PMID: 25756958
  95. Demonstrated that sonic hedgehog is a PRL-regulated cytokine in breast cancer cells and part of the mechanism that induces osteoclast differentiation. PMID: 26586670
  96. Aberrant activation of SHH-GLI signaling promotes upregulation of ABCG2 that plays key roles in the development of drug resistance. PMID: 26276718
  97. These findings revealed a novel role for Shh-Gli1 signals in epithelial-mesenchymal transition in ovarian cancer PMID: 25432698
  98. SHH mutations or copy number variations are not a common cause of congenital hypopituitarism in patients without complex midline cerebral defects PMID: 25056824
  99. A role of Shh in promoting glycolysis and proliferation of breast cancer cells via PFKFB3 phosphorylation PMID: 26171876
  100. from ESCs and induced pluripotent stem cells. SIGNIFICANCE STATEMENT: Our study presents a rapid and efficient protocol to generate human motoneurons from embryonic and induced pluripotent stem cells. PMID: 26290227

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Involvement in disease Microphthalmia, isolated, with coloboma, 5 (MCOPCB5); Holoprosencephaly 3 (HPE3); Solitary median maxillary central incisor (SMMCI); Triphalangeal thumb-polysyndactyly syndrome (TPTPS); Preaxial polydactyly 2 (PPD2); Hypoplasia or aplasia of tibia with polydactyly (THYP); Laurin-Sandrow syndrome (LSS)
Subcellular Location Sonic hedgehog protein N-product: Cell membrane, Lipid-anchor
Protein Families Hedgehog family
Database Links

HGNC: 10848

OMIM: 135750

KEGG: hsa:6469

STRING: 9606.ENSP00000297261

UniGene: Hs.164537

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