>98% as determined by SDS-PAGE and HPLC.
Less than 1.0 EU/μg as determined by LAL method.
Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine C3H/10T1/2 cells is less than 1 μg/ml, corresponding to a specific activity of >1.0x103 IU/mg.
HHG 1; HHG-1; HHG1; HLP 3; HLP3; Holoprosencephaly 3; HPE 3; HPE3; MCOPCB5; shh; SHH_HUMAN; SMMC I; SMMCI; Sonic Hedgehog (Drosophila) homolog; sonic hedgehog homolog (Drosophila); Sonic hedgehog homolog; Sonic hedgehog protein; Sonic hedgehog protein C-product; TPT; TPTPS
Homo sapiens (Human)
IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG
Lyophilized from a 0.2 µm filtered 20 mM PB, pH 7.4, 150mM NaCl
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
5-10 business days
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Recombinant Human SHH is a high-quality, tag-free partial protein corresponding to the 25-197aa expression region of the Sonic hedgehog protein, a critical component in cancer research. Expressed in E. coli, this product boasts remarkable purity, greater than 98% as determined by both SDS-PAGE and HPLC. With endotoxin levels under 1.0 EU/µg, as assessed by the LAL method, this protein ensures minimal interference in experimental setups. Fully biologically active when compared to the standard, SHH's ED50 is established by its ability to induce alkaline phosphatase production in murine C3H/10T1/2 cells at less than 1 µg/mL, yielding a specific activity of ＞ 1.0 × 103 IU/mg. The product is supplied as a lyophilized powder for ease of storage and application in various research contexts.