APEH Antibody

Datasheet

Code	CSB-PA001899LA01HU
Size	US$166
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Image	Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA001899LA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA001899LA01HU at dilution of 1:100 Immunofluorescent analysis of Hela cells using CSB-PA001899LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) Western Blot Positive WB detected in: Hela whole cell lysate All lanes: APEH antibody at 3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 82 kDa Observed band size: 82 kDa Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA001899LA01HU at dilution of 1:100
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) APEH Polyclonal antibody

Uniprot No.

P13798

Target Names

APEH

Alternative Names

AARE antibody; ACPH antibody; ACPH_HUMAN antibody; Acyl peptide hydrolase antibody; Acyl-peptide hydrolase antibody; Acylamino acid releasing enzyme antibody; Acylamino-acid-releasing enzyme antibody; Acylaminoacyl peptidase antibody; Acylaminoacyl-peptidase antibody; APEH antibody; APH antibody; N acylaminoacyl peptide hydrolase antibody; OPH antibody; Oxidized protein hydrolase antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Acylamino-acid-releasing enzyme protein (201-500AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The APEH Antibody (Product code: CSB-PA001899LA01HU) is Non-conjugated. For APEH Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA001899LB01HU	APEH Antibody, HRP conjugated	ELISA
FITC	CSB-PA001899LC01HU	APEH Antibody, FITC conjugated
Biotin	CSB-PA001899LD01HU	APEH Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:2000-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser.

Gene References into Functions

Although APEH is primarily localized in the cytoplasm, a sub-fraction of this enzyme is sequestered at sites of nuclear damage following UVA irradiation or following oxidative stress. APEH interacts with the amino-terminal domain of XRCC1, and APEH facilitates both single-strand break repair and cell survival following exposure to H2O2. Thus, APEH as a novel proteolytic component of the DNA damage response. PMID: 28866241
APEH polymorphism has significant influence on valproic acid pharmacokinetics in Chinese population PMID: 27406852
Studies indicate that the cylindromatosis/turban tumor syndrome gene (CYLD) ranked highest, followed by acylaminoacyl-peptidase (APEH), dystroglycan (DAG1), macrophage-stimulating protein (MST1) and ubiquitin-specific peptidase 4 (USP4). PMID: 21931648

Subcellular Location

Cytoplasm.

Protein Families

Peptidase S9C family

Database Links

HGNC: 586

OMIM: 102645

KEGG: hsa:327

STRING: 9606.ENSP00000296456

UniGene: Hs.517969

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Antibody Service

Protein Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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