NOX4 Antibody

Code CSB-PA015961LA01HU
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
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  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA015961LA01HU at dilution of 1:100

  • Immunofluorescent analysis of HepG2 cells using CSB-PA015961LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) NOX4 Polyclonal antibody
Uniprot No. Q9NPH5
Target Names NOX4
Alternative Names Kidney oxidase 1 antibody; Kidney oxidase-1 antibody; Kidney superoxide producing NADPH oxidase antibody; Kidney superoxide-producing NADPH oxidase antibody; KOX 1 antibody; KOX antibody; Kox-1 antibody; KOX1 antibody; NADPH antibody; NADPH oxidase 4 antibody; Nox4 antibody; NOX4_HUMAN antibody; Renal NAD(P)H oxidase antibody; Renal NAD(P)H-oxidase antibody; RENOX antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human NADPH oxidase 4 protein (210-424AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, IHC, IF
Recommended Dilution
Application Recommended Dilution
IHC 1:20-1:200
IF 1:50-1:200
Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

Wound Healing Promoting Activity of Tonsil-Derived Stem Cells on 5-Fluorouracil-Induced Oral Mucositis Model. Jung H, et al,Tissue Engineering and Regenerative Medicine,2019

Sample type: Syrian golden hamsters tissue section
Sample species: Mouse
Antibody dilution factor: 1:500
Application: IHC
Review: Immuno- histochemistry for TGF-b and NOX4 on days 10 and 14. On day 10, the TMSC group showed the highest TGF-b and NOX4 expression. *p<0.05, **p<0.01. 9 200.

Target Data

Function Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 5 and isoform 6 display reduced activity.; FUNCTION
Gene References into Functions
  1. Metabolic syndrome in Brazilian NAFLD patients most likely results from common allelic variants in a large number of genes, including CYBA and NOX4, that interact with each other, each of which alone determines a modest risk. PMID: 30087027
  2. This genome-wide association study of severe diabetic retinopathy suggests new evidence for the involvement of the NOX4 gene. PMID: 30178632
  3. These results suggest the specific involvement of Nox4 and Nox2 subunits as physiologically relevant endothelial sources of H2O2 generation that contribute to the endothelium-dependent vasodilatation of renal arteries and therefore have a protective role in kidney vasculature. PMID: 30125808
  4. NOX4 is induced in early alcoholic liver injury and regulates CCR2/CCL2 mRNA stability thereby promoting recruitment of inflammatory cells and production of proinflammatory cytokines. PMID: 28383062
  5. Study reveals the novel function of NOX4 in reprogramming aerobic glycolysis initiated by activated Kras and inactivated p16 in pancreatic ductal adenocarcinoma (PDAC), indicating its potential as a therapeutic target for PDAC and other cancers. PMID: 28232723
  6. Human post-mortem and animal studies have identified elevated NOX2 and NOX4 levels in the injured brain, suggesting that these two NOXs are involved in the pathogenesis of Traumatic brain injury (TBI) PMID: 29571125
  7. our data demonstrate that hypoxia strongly potentiates the peroxide-mediated induction of hepcidin via STAT3 signaling pathway. Moreover, oxidases such as NOX4 or artificially overexpressed urate oxidase (UOX) can induce hepcidin PMID: 29459227
  8. Nox4 and its linked ER stress were shown to mainly contribute to eNOS uncoupling and its associated signaling in endothelial cells PMID: 28916474
  9. Our data suggest that TGF-beta1-induced chemokinesis in PDAC cells is mediated through a RAC1/NOX4/ROS/p38 MAPK cascade. PMID: 29039574
  10. GATA4 may inhibit diabetesinduced endothelial dysfunction by acting as a transcription factor for NOX4 expression. PMID: 29138836
  11. The results demonstrate that the heightened sensitivity of the brain to ischemic damage is due to an organ-specific role of NOX4 in blood-brain barrier endothelial cells and neurons. PMID: 29087944
  12. PTEN inhibits replicative senescence-induced MMP-1 expression by regulating NOX4-mediated reactive oxygen species in human dermal fibroblasts. PMID: 28557373
  13. The NADPH-dependent reduction of cytochrome c or cytochrome b5 by purified Nox4 DH domain was found regulated by the H2O2 concentration, and C546L and C547L mutants showed lower rates of the hemeprotein reduction. PMID: 29365138
  14. We demonstrated that small interfering RNA (siRNA)-mediated knockdown of PRR, Nox2 and Nox4 significantly reduced the HG-induced stimulation of VEGF. On the other hand, Nox4 overexpression significantly potentiated PRR-induced stimulation of VEGF under hyperglycemia in ARPE-19 cells. PMID: 28840773
  15. Studies indicate that NADPH oxidase 4 (Nox4) is present in fibroblasts, a primary cell of the adventitia, and matches the adventitial location of ROS production in pulmonary arterial hypertension [Review]. PMID: 29047077
  16. Serum Gal-3 and Nox4 levels were significantly elevated and correlated in 26 human pulmonary arterial hypertension patients when compared with 14 age- and sex-matched healthy controls. PMID: 28431936
  17. we demonstrated that when NOX4 expression was knocked down by siRNAs, cell proliferation, cell-cycle and apoptosis, migration and invasion were significantly altered in CRC cell lines HCT116 and LOVO. Meanwhile, NOX4 promoted cancer cell proliferation and apoptosis, migration and invasion by regulating the expression of relevant genes. PMID: 28422720
  18. Nox4 not only mediated TGF-beta1- induced collagen type I synthesis but also the expression of the myofibroblast markers alpha-SMA and fibronectin 1. PMID: 27576129
  19. High NOX4 expression is associated with drug resistance in renal cell carcinoma. PMID: 29051480
  20. Nox4 potentially mediates HGinduced HKC cell apoptosis. PMID: 28487945
  21. Our results showed that lowering NOX4 oxidase below physiological level leads to cellular senescence of vascular smooth muscle cells PMID: 27655718
  22. Extracellular advanced oxidative protein products accumulation triggered NOX4-dependent reactive oxygen species production, which activated ERK1/2 and p38 MAPK, and induced HaCaT cell apoptosis by activating caspase 3 and PARP-1. PMID: 27155970
  23. The results establish a link between BRAF(V600E) and NOX4, which is confirmed by a comparative analysis of NOX4 expression in human (TCGA) and mouse thyroid cancers. PMID: 27401113
  24. This study also showed that UCH-L1 promotes angiogenesis of HUVECs, as well as invasion in cancer cells, by up-regulating ROS by deubiquitination of NOX4, suggesting that UCH-L1 plays a key role in angiogenesis of HUVECS by regulating ROS levels by deubiquitination of NOX4. PMID: 29128359
  25. The results reveal that NOX4 promotes glycolysis, contributing to non-small cell lung cancer cells growth, and supports glutaminolysis for oxidative resistance. PMID: 27989748
  26. Metformin attenuates idiopathic lung fibrosis development via suppression of myofibroblast NOX4 expression. PMID: 27576730
  27. Letter: airway smooth muscle cell NOX4 expression is increased in vivo and in vitro in COPD. PMID: 27435477
  28. These data suggested that t-BHP induced both apoptosis and necroptosis in endothelial cells which was mediated by ROS and p38MAPK. ROS derived from NADPH oxidase and mitochondria contributed to t-BHPL and t-BHPH-induced apoptosis and necroptosis, respectively PMID: 28088644
  29. These processes are mediated upstream by the reactive oxygen species (ROS)-producing enzyme Nox4. PMID: 28182006
  30. data suggest that monocytic Nox4 is a central regulator of actin dynamics, and induction of Nox4 is the rate-limiting step in metabolic stress-induced monocyte priming and dysfunction associated with accelerated atherosclerosis and the progression of atherosclerotic plaques PMID: 23825596
  31. findings demonstrate that manipulation of the host PI3K/Akt signaling pathway and Nox4 gene expression is a novel mechanism involved in T. gondii survival and proliferation PMID: 23824914
  32. Loss of NOX4 increases actomyosin levels and favours an epithelial to amoeboid transition contributing to tumour aggressiveness. PMID: 27941881
  33. NOX2, NOX4, and mitochondrial-derived reactive oxygen species contribute to angiopoietin-1 signaling and angiogenic responses in endothelial cells. PMID: 28351775
  34. Mechanistically, HO-1 induction by all CRLPs requires NADPH oxidase 4, with PUFA-containing particles additionally dependent upon mitochondrial reactive oxygen species.These studies define new molecular pathways coupling endothelial cell activation by model CMRs with adaptive regulation of Nrf2-dependent HO-1 expression PMID: 27185859
  35. NOX4 knockout cell lines showed reduced cell proliferation with an increase of sub-G1 cell population and the decrease of S/G2/M population, and resulted in a dramatic decrease in invadopodium formation and the invasive activity. NOX4 deficiency caused a decrease in focal adhesions and cell migration in HeLa cells. The results suggest that NOX4 is required for both efficient proliferation and invasion of HeLa cells. PMID: 28099519
  36. Thioredoxin attenuates oxidized low-density lipoprotein induced oxidative stress in human umbilical vein endothelial cells by reducing NOX2-NOX4 activity. PMID: 28688762
  37. Nox4-derived H2O2 in part activates Nox2 to increase mitochondrial ROS via pSer36-p66Shc, thereby enhancing VEGFR2 signaling and angiogenesis in endothelial cells. PMID: 28424170
  38. TGF-beta1 increases NADPH oxidase 4 (NOX4) mRNA and protein expression in normal human lung fibroblasts (NHLFs) and causes nuclear export of HDAC4. PMID: 28336812
  39. Nox4 should contribute to the pathological processes insubarachnoid hemorrhage(SAH)-induced early brain injury (EBI), and there was not an overlay effect of Nox2 inhibition and Nox4 inhibition on preventing SAH-induced EBI. PMID: 28330417
  40. TGFbeta1 was found to induce Nox4 mRNA expression and total collagen release by these cells (P < 0.05; n = 4), and both responses are blocked by Smad3 inhibitor SIS3. Suppressing Nox4 gene transcription with Adv-Nox4i completely attenuated TGFbeta1-stimulated H2O2 release and collagen production by conjunctival fibroblasts PMID: 28605812
  41. CD44V6 is part of a positive-feedback loop with TGFbeta1/TGFbetaRI signaling that acts to increase NOX4/ROS production, which is required for myofibroblast differentiation, myofibroblast differentiation, myofibroblast extracellular matrix production, myofibroblast invasion, and myofibroblast contractility. PMID: 28389561
  42. Endoplasmic reticulum stress triggers a localized signaling module on the ER surface involving Nox4-dependent calcium mobilization, which directs local Ras activation through ER-associated, calcium-responsive RasGRF. PMID: 27856453
  43. PI3K/AKT signaling only occurs when FLT3-ITD is expressed at the plasma membrane and is required for the production of NOX-generated ROS. ER retention of FLT3-ITD resulted in NOX4 deglycosylation and p22(phox) protein degradation. PMID: 27870947
  44. NOX4 upregulation confers anoikis resistance. PMID: 28081539
  45. these data demonstrate that increased expression and activation of NOX4, which might result from increased TGFbeta1 levels seen during aging, induces a proinflammatory phenotype in VSMCs, enhancing atherosclerosis. PMID: 27986445
  46. The ROS levels of the study group decreased obviously before irradiation (P<0.01), however, the radiation-induced ROS of the study group was at a high level even when irradiation had been terminated for 2 h (P<0.01). Moreover, NOX2 and NOX4 levels and total SOD activity decreased (P<0.01), while the levels of SOD1 were stably maintained (P>0.05). PMID: 28260074
  47. alkali burns markedly upregulated the transcription and expression of Nox2 and Nox4 in human or mouse corneas. PMID: 27221536
  48. The NOX4 and TLR2 pathways played important roles in the biological effects mediated by Bletilla striata polysaccharide b. PMID: 27151672
  49. it was demonstrated that elevated uric acid promoted ROSinduced tubular cell apoptosis by upregulating Nox4 expression. PMID: 27052425
  50. expression of MATER and NOX4 proteins are closely related to the follicular development and ovulation with particular regard for ovarian aging PMID: 27515505
  51. Data indicate that endothelin receptor antagonist CPU0213 has an anti-apoptosis role through NADPH oxidase 4 (Nox4)-dependent O2-.production. PMID: 27336467
  52. Oleic/[almitic acid treatment enhances reactive oxygen species production and cell apoptosis mainly by upregulating the protein levels of NOX4 and caspase3 activation in chondrocytes. PMID: 27466293
  53. miR-99a directly regulates the invasion and migration in lung adenocarcinoma by targeting NOX4. PMID: 26986073
  54. of smooth muscle Nox4 inhibits atherosclerosis by suppressing sEH, which, at least in part, accounts for inhibition of SMC proliferation, migration and inflammation PMID: 26812119
  55. Data suggest that NOX4 and mitochondrial oxidative stress are mediators of cardiovascular disease in aging under hyperlipidemic conditions. PMID: 26054376
  56. Downregulation of smooth muscle Nox4 inhibits neointimal hyperplasia by suppressing TSP1. PMID: 26582463
  57. High expression of NOX4 is associated with metastasis in prostate cancer. PMID: 26473288
  58. The angiogenic effect of TRAIL on human microvascular endothelial cell-1 cells is downstream of fibroblast growth factor-2, involving NOX4 and nitric oxide signaling. PMID: 26572549
  59. A deep understanding of Nox4 structure/function and mechanisms of regulation could lead both to the identification of new therapeutic targets and to the development of innovative strategies for appropriate osteoarthritis treatment. [review] PMID: 27509686
  60. Expression of NOX4/p22(phox) as well as ROS production is enhanced by IL-1beta. On the other hand, the use of NOX4 inhibitors decreased IL-1beta-induced collagenase synthesis by chondrocytes. PMID: 26521743
  61. Nox4 is a positive transcriptional regulator of cystathionine-gamma-lyase in endothelial cells. PMID: 26620565
  62. An endogenous NOX4 forms macromolecular complexes with calnexin, which are needed for the proper maturation, processing, and function of NOX4 in the endoplasmic reticulum. PMID: 26861875
  63. IFNgamma induces oxidative stress, DNA damage and tumor cell senescence via TGFbeta/SMAD signaling-dependent induction of Nox4 and suppression of ANT2 PMID: 25982278
  64. Nox4 knockout (Nox4(-/-)) murine hematopoietic progenitor cells were refractory to FLT3ITD-mediated transformation in vitro PMID: 26308771
  65. H2O2-forming NOX4, unlike the superoxide-forming NOX1, can act as a negative modulator of inflammation and remodeling and convey atheroprotection. PMID: 26715682
  66. results demonstrate that in VSMCs mechanical stimulation activates cofilin by a Nox4-dependent mechanism and that this pathway is required for cytoskeleton reorganization and cell reorientation PMID: 25998423
  67. our results indicate that Nox4-mediated ROS, at least in part, transmit cell survival signals and their depletion leads to apoptosis, thus highlighting the Nox4-ROS-AKT signaling pathway as a potential therapeutic target for MPM treatment. PMID: 26238284
  68. High glucose generated an increase in NADPH oxidase activity and expression in human vascular smooth muscle cells. Sequence analysis of human Nox1, Nox4, and Nox5 gene promoters PMID: 25722086
  69. results suggest the evaluated NOX4 and CYBA SNPs are not direct genetic determinants of fibrosis in patients with chronic hepatitis C, but nevertheless NOX4 rs3017887 SNP could indirectly influence fibrosis susceptibility due to its inverse association with metabolic syndrome in male patients PMID: 25888935
  70. Via increased or decreased generation of reactive oxygen species and/or hydrogen peroxide, Nox4 could orchestrate collagen synthesis, differentiation of dermal fibroblasts into a profibrotic myofibroblast phenotype and thus dermal fibrosis. PMID: 25040787
  71. c-Src/AKT is the upstream signaling that regulates TGIF-induced Nox4 activation and subsequent superoxide production. PMID: 25841779
  72. Fluid shear stress regulates PLGF expression via NADPH oxidase 4 and reactive oxygen species signaling. PMID: 26408539
  73. There is involvement of NOX-4 in systemic sclerosis-associated fibrosis, and it indicates NOX-4 inhibitors as novel therapeutic approaches for SSc. PMID: 26096997
  74. the findings of the present study suggest that H2O2 contributes to HUVEC apoptosis by inducing Nox4-dependent ROS aggregation and activating the TGF-beta1/Smad2 signaling pathway. PMID: 25891879
  75. AngII/AT1/Nox4 axis-mediated oxidative stress gives rise to the dopamine neuron dysfunction and loss characteristic of the neuropathological and clinical manifestations of PD. PMID: 25645462
  76. results indicate a critical role for histone modifications involving H4K16Ac in epigenetic activation of the Nox4 gene, while the role of DNA methylation may be contextual PMID: 25526894
  77. Expression of extracellular superoxide dismutase (EC-SOD) and expression of the prooxidant gene NADPH oxidase 4 was decreased significantly by trichostatin A. PMID: 25749103
  78. Nox4 promotes retinal neovascularization through H2O2/VEGFR2/ERK signaling pathway PMID: 25866826
  79. It is likely that the Smad-independent ERK1/2 pathway regulates Nox4 expression and may be involved in the TGFB1-induced proliferation of endothelial cells PMID: 25428269
  80. PPARgamma plays a central role in the regulation of the ERK1/2-NF-kappaB-Nox4-H2O2 signaling axis in pulmonary artery smooth muscle cell proliferation. PMID: 25557278
  81. Data show that NOX4/Akt and IL-6/STAT3 signalings can reciprocally and positively regulate each other, leading to enhanced non-small cell lung cancer (NSCLC) cell proliferation and survival. PMID: 25504436
  82. Data show that tacrolimus-induced NAD(P)H-oxidase 4 (Nox4) expression in by aberrant TGF-beta receptor signalling. PMID: 24816588
  83. NOX2 and NOX4 expressions significantly increase at an early stage after traumatic brain injury, and abnormal expressions of NOX2 and NOX4 are correlated to patient prognosis to certain extent PMID: 25079350
  84. These data demonstrate that influenza A virus infection of lung epithelial cells causes a significant reactive oxygen species increase that depends mainly on NOX4, which is upregulated at both mRNA and protein levels. PMID: 25154738
  85. NOX4 generates reactive oxygen species (ROS) in systemic sclerosis (SSc) fibroblasts and plays a critical role in cell activation and DNA damage. Expression of NOX4 in SSc fibroblasts is maintained by a ROS-mediated loop. PMID: 25707572
  86. The role of Nox-4 as it pertains to kidney disease, with a particular emphasis on diabetic nephropathy, is reviewed. PMID: 25402870
  87. Upregulation of Nox4 in the myocardium causes cardiac remodeling through activating Akt-mTOR and NF-kappaB signaling pathways. PMID: 25589557
  88. NOX4 is highly predictive of relapse in stage II left-side colon cancer, whereas integrin alpha 3 beta 1 (ITGA3) is predictive of relapse in stage II right-side colon cancer. PMID: 25096929
  89. Data show that NADPH oxidase NOX4 and p22(phox) localize to the nuclear membrane in MV4-11 leukemia cells expressing internal tandem duplication of the FMS-like tyrosine kinase (FLT3-ITD) receptor. PMID: 25697362
  90. AMPK inactivation with NOX4-induced ROS formation and transforming growth factor ss-1 (TGFss-1) signaling activation. PMID: 25278128
  91. we show here for the first time that two members of the ROS-generating NADPH oxidase family (NOXs), NOX4 and NOX5, are involved in radiation-induced DNA damage. PMID: 25706776
  92. conclude that, in A-T disease in humans and mice, NOX4 may be critical mediator and targeting it will open up new avenues for therapeutic intervention in neurodegeneration PMID: 25646414
  93. Data indicate that downregulation of endothelial NADPH oxidase 4 (Nox4) decreases transforming growth factor beta1 (TGFbeta1). PMID: 25315297
  94. Expression of NOX4 increased in uterine fibroid tissue compared to normal myometrial tissues and cells. PMID: 24520084
  95. Demonstrate efficacy of antioxidants in the prevention of endothelial dysfunction is mediated by the reduction of inflammatory cell recruitment through the inhibition of Nox4-mediated ROS generation and the restoration of mitochondrial beta-oxidation. PMID: 25401479
  96. Studies indicate that NADPH oxidase 4 protein (Nox4) promotes the activation of autophagy and survival in cardiomyocytes in response to nutrient deprivation and ischaemia. PMID: 25515000
  97. Nox4 is a major superoxide-producing enzyme and its expression is regulated by Ang II and hypoxic stress in human brain pericytes. PMID: 25612841
  98. Nox4 NADPH oxidase may be an important downstream effector in mediating TGF-beta-induced fibrosis, while NADPH oxidase-dependent redox signaling may in turn regulate TGF-beta/Smad signaling in a feed-forward manner. Review. PMID: 24494202
  99. miR-17 regulated the expression of NOX2 and NOX4, which in turn regulated the reactive oxygen species production in microglial cells PMID: 25146963
  100. Loss of Trx-1 and upregulation of NOX4 contribute to the imbalance in the redox-status of senescent endothelial cells ex vivo and in vivo. PMID: 24632182

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Subcellular Location Endoplasmic reticulum membrane, Multi-pass membrane protein, Cell membrane, Multi-pass membrane protein, Cell junction, focal adhesion
Tissue Specificity Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level). Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelial cells, skeletal muscle, brain, several brain t
Database Links

HGNC: 7891

OMIM: 605261

KEGG: hsa:50507

STRING: 9606.ENSP00000263317

UniGene: Hs.371036

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