P2RX4 Antibody

Code CSB-PA859513LA01HU
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
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  • Western Blot
    Positive WB detected in: Mouse brain tissue, Mouse kidney tissue, Mouse liver tissue, Rat lung tissue, Rat skeletal muscle tissue
    All lanes: P2RX4 antibody at 3μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 44, 46, 41 kDa
    Observed band size: 44 kDa

  • Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA859513LA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA859513LA01HU at dilution of 1:100

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) P2RX4 Polyclonal antibody
Uniprot No. Q99571
Target Names P2RX4
Alternative Names AI504491 antibody; ATP gated cation channel protein antibody; ATP receptor antibody; AW555605 antibody; D5Ertd444e antibody; fi03f02 antibody; MGC143034 antibody; OTTMUSP00000023741 antibody; OTTMUSP00000023745 antibody; OTTMUSP00000023746 antibody; OTTMUSP00000023747 antibody; P2rx4 antibody; P2RX4_HUMAN antibody; P2X purinoceptor 4 antibody; P2X receptor subunit 4 antibody; P2X4 antibody; P2X4R antibody; Purinergic receptor antibody; Purinergic receptor P2X ligand gated ion channel 4 antibody; Purinergic receptor P2X4 antibody; Purinoceptor P2X4 antibody; wu:fi03f02 antibody
Raised in Rabbit
Species Reactivity Human, Mouse, Rat
Immunogen Recombinant Human P2X purinoceptor 4 protein (91-176AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated

The P2RX4 Antibody (Product code: CSB-PA859513LA01HU) is Non-conjugated. For P2RX4 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA859513LB01HU P2RX4 Antibody, HRP conjugated ELISA
FITC CSB-PA859513LC01HU P2RX4 Antibody, FITC conjugated
Biotin CSB-PA859513LD01HU P2RX4 Antibody, Biotin conjugated ELISA
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin.
Gene References into Functions
  1. Three single nucleotide polymorphisms (SNPs) within P2X4R and two SNPs within CAMKK2 influenced concentrations of TNFalpha in peripheral blood mononuclear cells, but these SNP did not associate with risk for HIV-associated sensory neuropathy in South Africans. PMID: 29428485
  2. the data reveals a role for P2X4 in determining the duration of ATP-evoked Ca(2+) responses and CXCL5 secretion in human primary macrophage. PMID: 29255078
  3. the data provides evidence that P2X4 is functionally expressed in THP-1 differentiated macrophages as reflected from their contribution towards ATP-evoked Ca2+ response, but their functional evidence in THP-1 monocyte is lacking. PMID: 29077063
  4. Our study nominates rare genetic variants in P2RX4 and P2RX7 as major genetic contributors to disease, further supporting a role for these purinergic receptors in MS and the disruption of transmembrane cation channels leading to impairment of phagocytosis as the pathological mechanisms of disease PMID: 28326637
  5. Overexpressing P2X4Rs on microglia are a central player in evoking neuropathic pain. (review) PMID: 28458288
  6. the intersubunit physical couplings among the DF and two lower body domains fostered by the LF domain at the open state act as an integrated structural element that is stringently required for the channel gating of P2X4 receptors. PMID: 28302727
  7. These findings provide new insights in understanding the contribution of the salt bridge between Asp-85 and Arg-309 and its structurally coupled beta2,3-sheet to the function of P2X4 receptors. PMID: 26865631
  8. The results suggest a role of PrP(C) in proteostasis, dysfunctions of which may be involved in the pathogenesis of neurodegenerative diseases such as TSE and Alzheimer's Disease. PMID: 26946358
  9. Fndings support role for P2X7 and P2X4 coupled to induction of inflammatory molecules in modulating high glucose and palmitate-induced endothelial cell activation and dysfunction. PMID: 25938443
  10. This study demonstrates a major physiological finding that the shear-induced effects on endothelial KLF2 axis are in part dependent on ATP release and P2X4, a previously unidentified mechanism. PMID: 25563726
  11. Using pHluorin, P2X4 was found to be expressed on the plasma membrane and within subcellular compartments in hippocampus. PMID: 24935743
  12. It appears to mediate the cells' response to extracellular ATP. Although Ca2thorn influx via P2X1 receptor is necessary for alpha-synuclein accumulation. PMID: 25480524
  13. P2X4 and calmodulin form a complex at endolysosomal membrane where P2X4 activation recruits calmodulin to promote fusion and vacuolation in a Ca(2+)-dependent fashion. PMID: 26101220
  14. the lysosome-localized P2X4 may play specific roles in membrane trafficking of acidic organelles in mammalian cells. PMID: 24817123
  15. P2X4 assembles with P2X7 and pannexin-1 in gingival epithelial cells and modulates ATP-induced reactive oxygen species production and inflammasome activation during P. gingivalis infection. PMID: 23936165
  16. Results suggest that ATP-P2X4 signaling mediates high glucose-induced activation of the NLRP3 inflammasome. PMID: 23434541
  17. These findings highlight Rab5 GTPase as a key regulator of P2X4 receptor cell surface expression and internalisation PMID: 23086000
  18. This is the first study demonstrating an association of non-synonymous polymorphisms in the P2RX ( 4 ) and the risk of osteoporosis, suggesting a role of the P2X ( 4 ) R in the regulation of bone mass PMID: 23138503
  19. results demonstrate that a haplotype including two rare variants in P2RX7 and P2RX4 confers a functional interaction between these two variant receptors that impairs the normal scavenger function of macrophages and microglia PMID: 23303206
  20. IL-18 associates to microvesicles shed from human macrophages by a LPS/TLR-4 independent mechanism in response to P2X receptor stimulation. PMID: 22996386
  21. The present article highlights the recent advances in our understanding of microglia-neuron interactions in neuropathic pain by focusing on the signaling and regulation of the P2X4R--{REVIEW} PMID: 22528681
  22. We demonstrate here that stimulation with ADP or ATP induces significant reversible inhibition of C. trachomatis development in cervical epithelial cells through stimulation of the purinergic receptor P2X4. PMID: 22988022
  23. found that pharmacological inhibition of P2X4 receptors with TNP-ATP inhibited transcriptional up-regulation of TNF-alpha and IFN-gamma in gammadelta T cells stimulated with anti-CD3/CD28-coated beads PMID: 22753954
  24. Neuropathic pain may be driven by P2X4R+ microglia. PMID: 22837036
  25. Allosteric modulation of Ca2+ flux in ligand-gated cation channel (P2X4) by actions on lateral portals PMID: 22219189
  26. Transcripts of five different isoforms of P2X4 receptors are expressed in human liver cells indicating that they represent an important component of the purinergic signaling complex in HCV induced liver pathogenesis. PMID: 21899776
  27. The Tyr315>Cys mutation (rs28360472) reduced the peak amplitude of the ATP-induced inward current. In Victorian Family Heart Study participants, 1 minor allele increased pulse pressure by 2.84 mm Hg. PMID: 22068874
  28. Owing to their favorable diameters and equivalent spacing, the lateral portals split the task of ion supply threefold and minimize an ion's diffusive path before it succumbs to transmembrane electrochemical gradients PMID: 21808018
  29. we have identified an extracellular signaling pathway where Tbeta4 increases cell surface ATP levels via ATP synthase and have shown further that ATP-responsive P2X4 receptor is required for Tbeta4-induced cell migration PMID: 21106936
  30. These data have identified the first transcription factor involved in P2X receptor expression. PMID: 19953327
  31. Results show that P2X4 and P2X6 receptors are associated with VE-cadherin at HUVEC adherens junctions. PMID: 12088286
  32. shear stress stimulates pulmonary artery endothelial cells to release ATP, which activates Ca2+ influx via P2X4 receptors. PMID: 12714321
  33. We have identified a distinct subpopulation of P2X4-positive macrophages infiltrating the dystrophic fibres. These cells were absent from normal muscle and rarely present in the dystrophic muscle taken before and after the onset of degeneration. PMID: 15006691
  34. Increased contractility likely underlies survival benefit from P2X4 receptor overexpression. PMID: 15130891
  35. in human parotid acinar cells, in addition to modulation of Ca(2+) release, Ca(2+) influx through P2X(4)R may constitute a further locus for the synergistic effects of Ca(2+) and PKA activation. PMID: 15262999
  36. wild-type desensitization properties requires an aromatic moiety at position 374 and an amino rather than a guanidino group at position 373 PMID: 16533808
  37. P2X4 receptor-specific residues contribute to the ivermectin effects on channel deactivation PMID: 16949036
  38. Main role for P2X(4) receptor in nucleotide-induced apoptosis in human mesangial cells, indicating a relevant role for purinergic signaling in regulating death rate in these cells. PMID: 17264311
  39. This review discusses the need to reinterpret the assumed "go-it alone" function of the P2X7 receptor in light of convincing biochemical and electrophysiological evidence for the existence of P2X4/P2X7 heteromeric receptors. PMID: 17895406
  40. support a common site of ATP action at P2X receptors and suggest that non-conserved residues also play a regulatory role in agonist action PMID: 18487206
  41. analysis of molecular shape, architecture, and size of P2X4 receptors PMID: 18635539
  42. INF-gamma selectively increases P2X4-receptor gene expression, leading to an up-regulation of purinergic signaling in vascular endothelial cells. PMID: 18678988
  43. P2X(4) and P2X(7) receptors are expressed by human osteoblast-like cells. P2X(7) receptor is mainly responsible for pore formation although P2X(4) receptors may also be involved. PMID: 19226284
  44. This study presents a picture whereby P2X(4)R become functional in response to initial phagocytic stimuli but return to a non-functional state during sustained activation by classical macrophage activation. PMID: 19283779
  45. Results suggest that amyloid beta(1-42)-induced synaptic dysfunction and neuronal death may involve perturbations in P2X4 purinergic receptors. PMID: 19562525

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Subcellular Location Membrane; Multi-pass membrane protein.
Protein Families P2X receptor family
Database Links

HGNC: 8535

OMIM: 600846

KEGG: hsa:5025

STRING: 9606.ENSP00000336607

UniGene: Hs.321709

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