PCK1 Antibody

Code CSB-PA017613LA01HU
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
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  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA017613LA01HU at dilution of 1:100

  • Immunofluorescent analysis of Hela cells using CSB-PA017613LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) PCK1 Polyclonal antibody
Uniprot No. P35558
Target Names PCK1
Alternative Names cytosolic [GTP] antibody; GTP antibody; PCK1 antibody; PCKGC_HUMAN antibody; PEP carboxykinase antibody; PEPCK-C antibody; PEPCK1 antibody; PEPCKC antibody; Phosphoenolpyruvate carboxykinase 1 (soluble) antibody; Phosphoenolpyruvate carboxykinase 1 antibody; Phosphoenolpyruvate carboxykinase antibody; Phosphoenolpyruvate carboxykinase, cytosolic [GTP] antibody; Phosphoenolpyruvate carboxykinase, cytosolic antibody; Phosphoenolpyruvate carboxylase antibody; Phosphopyruvate carboxylase antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Phosphoenolpyruvate carboxykinase, cytosolic [GTP] protein (1-622AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, IHC, IF
Recommended Dilution
Application Recommended Dilution
IHC 1:20-1:200
IF 1:50-1:200
Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

Propionate suppresses hepatic gluconeogenesis via GPR43/AMPK signaling pathway. Yoshida H, et al,Archives of Biochemistry and Biophysics,2019

Applications: WB
Review: Protein levels of G6Pase and PEPCK in propionate-treated HepG2 cells. The cells were incubated with the indicated concentrations of propionate in the presence or absence of 0.5 mM palmitate for 24 h. After incubation, the protein levels of G6Pase (C) and 15 PEPCK (E) were determined by immunoblotting. Band densities of G6Pase (D) and PEPCK (F) were quantified and normalized for β-actin, and expressed as fold change versus vehicle-treated control.
PMID: 31356781

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Target Data

Function Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
Gene References into Functions
  1. This study reveals a unique mechanism to suppress hepatocellular carcinoma by switching from glycolysis to gluconeogenesis through Nur77 antagonism of PEPCK1 degradation. PMID: 28240261
  2. circC3P1 acts as a tumor suppressor via enhancing PCK1 expression by sponging miR-4641 in hepatocellular carcinoma. PMID: 29608893
  3. The expression levels of PCK1 were suppressed in hepatocellular carcinoma (HCC) samples and in cells derived from HCC tissues. PMID: 29768256
  4. Study investigated the molecular basis of such effects focusing on a commonly studied polymorphism in pig Pgc1alpha, which changes a cysteine at position 430 (WT) of the protein to a serine (C430S); found that differential O-GlcNAcylation of Pgc1alpha regulates PCK1 activity and this molecular mechanism could explain at least in part the epistatic interaction between both genes. PMID: 28644880
  5. Study identified a novel homozygous PCK1 missense mutation causing cytosolic phosphoenolpyruvate carboxykinase deficiency presenting as childhood hypoglycemia. PMID: 28216384
  6. HBXIP is able to depress the gluconeogenesis in hepatoma cells by suppressing PCK1 to promote hepatocarcinogenesis, involving miR-135a/FOXO1 axis and PI3K/Akt/p-FOXO1 pathway. PMID: 27609066
  7. an extended binding site in the catalytic cleft of cPEPCK which is used by 3-MPA to inhibit cPEPCK non-competitively. PMID: 26528723
  8. A mutation of PCK1 associated with Smith-Magenis Syndrome, cytosolic PEPCK deficiency and NMDA receptor glutamate insensitivity. PMID: 24863970
  9. Insulin-dependent translocation of FOXO1 regulated transcriptional repression of PCK1 concomitant with the formation of the FOXO1-euchromatic histone-lysine N-methyltransferase2 (EHMT2) complex and histone modifications of the PCK1 promoter region PMID: 25736587
  10. Lipopolysaccharide and monophosphoryl lipid A also up-regulated G6PC and PCK1 transcript abundance in a TLR4-dependent manner. PMID: 23465595
  11. The PCK1 tag-single nucleotide polymorphisms influenced insulin resistance by interacting with plasma n-3 polyunsaturated fatty acids levels in metabolic syndrome patients. PMID: 23092637
  12. HCV core protein expression-mediated FOXO1 activation and the increased PGC-1alpha leaded to the elevation of PCK1 at the mRNA level PMID: 22489460
  13. Acetylation regulates the stability of the gluconeogenic rate-limiting enzyme PEPCK1. PMID: 21726808
  14. Data show PEPCK-C and CPT-1 mRNAs are more abundant in non-tumoral tissues than in the tumoral counterpart, whereas the opposite occurred for the FAS gene. PMID: 20691246
  15. Studies demonstrate the association between PCK1 and smaller average brain volume. PMID: 21152065
  16. Study in the EYHS cohort failed to identify an association of PCK1 polymorphisms with obesity, physical activity, and fitness. PMID: 20134411
  17. crystal structure of human cytosolic phosphoenolpyruvate carboxykinase reveals a new GTP-binding site PMID: 11851336
  18. Conserved amino acids within CCAAT enhancer-binding proteins (C/EBP(alpha) and beta) regulate gene expression PMID: 11997389
  19. promoter context may influence which domains within a transcription factor are employed to mediate transactivation of PEPCK PMID: 12237288
  20. the PGC- 1-regulated phospho(enol)pyruvate carboxykinase (PEPCK) promoter is a novel target promoter for SHP inhibition PMID: 12324453
  21. transcription of the gene for phosphoenolpyruvate carboxykinase (GTP) in the liver is regulated by SREBP-1c and Sp1 PMID: 14744869
  22. Promoter single nucleotide polymorphism in PCK1 was resistant to down-regulation by insulin in vitro and associated with type 2 diabetes mellitus. PMID: 14764811
  23. PCK1 has been implicated as one of many genes associated with type 2 diabetes mellitus [review] PMID: 15046742
  24. AUF1 binds to multiple destabilizing elements within the 3'-UTR that participate in the rapid turnover of the phosphoenolpyruvate carboxykinase mRNA. PMID: 15951444
  25. The multiple instability elements present within the 3'-UTR may function synergistically to mediate both the rapid degradation and the cAMP-induced stabilization of PEPCK mRNA. The latter process may result from a PKA-dependent phosphorylation of AUF1. PMID: 16144962
  26. Subjects with the PCK1-232G/G genotype had more carotid IMT (0.80+/-0.02 versus 0.73+/-0.03 mm; P=0.007) but less TPV (0.10+/-0.09 versus 0.38+/-0.13; P=0.03) than subjects with other genotypes. PMID: 16282543
  27. Significant association of single nucleotide polymorphism within three genes--PPARgamma, SOS2, and PCK1--with Alzheimer's disease, was confirmed. PMID: 17440948
  28. IFN-gamma exerts a tissue-specific action in rodents and humans, having glyceroneogenesis and the PEPCK-C gene as selective targets to intensify fatty acids release from adipocytes PMID: 17495004
  29. The effects of several variants of retinoic acid on the expression and activity of PEPCK in human, mouse, and rat adipose tissue are reported. PMID: 18492826
  30. Reduced CREB phosphorylation (Ser-129) associated with inactivation of GSK3beta by Ser-9 phosphorylation may be the major mechanism underlying PEPCK-C gene suppression by AMPK-activating agents such as biguanide PMID: 18801732
  31. The aromatic ring of Tyr235 in PEPCK helps to position PEP in the active site and the hydroxyl group allows an optimal PEP-Mn(2+) distance for efficient phosphoryl transfer and overall catalysis. PMID: 19021757
  32. Val184 of PCK1 gene might increase the risk of type 2 diabetes in eastern Chinese population with BMI<23 kg/m(2) via reducing the PEPCK-C activity. PMID: 19070910
  33. Study validated a predicted SP1 binding site in the control of PCK1 transcription using gel shift and reporter assays. PMID: 19329064
  34. study is the first to investigate the association between variants of the PCK1 gene and type 2 diabetes in South Asians PMID: 19725958

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Involvement in disease Phosphoenolpyruvate carboxykinase deficiency, cytosolic (PCKDC)
Subcellular Location Cytoplasm
Protein Families Phosphoenolpyruvate carboxykinase [GTP] family
Tissue Specificity Major sites of expression are liver, kidney and adipocytes.
Database Links

HGNC: 8724

OMIM: 261680

KEGG: hsa:5105

STRING: 9606.ENSP00000319814

UniGene: Hs.1872

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