Phospho-CHUK/IKBKB (S176/177) Antibody

Code CSB-PA006829
Size US$167
  • Western Blot analysis of NIH-3T3 cells using Phospho-IKKα/β (S176/177) Polyclonal Antibody

Product Details

Uniprot No. O15111O14920
Target Names CHUK/IKBKB
Alternative Names CHUK; IKKA; TCF16; Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; IKK1; Nuclear factor NF-kappa-B
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Immunogen Synthesized peptide derived from Human IKKα/β around the phosphorylation site of S176/177.
Conjugate Non-conjugated
Isotype IgG
Purification Method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form Liquid
Tested Applications WB, IHC, ELISA
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
Protocols Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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Oral glutamine supplementation attenuate inflammation and oxidative stress-mediated skeletal muscle protein content degradation in immobilized rats: Role of 70 kDa heat shock protein. Petry ER, et al,Free Radical Biology and Medicine,2019

Applications: WB
Antibody dilution factor: 1: 1000
Review: Western blot analysis of total NF-kB p65 (A), phosphorylated NF-kB p65 (B), total IKKα/β (C) and phosphorylated IKKα/β (D) protein expression in soleus muscle of Wistar rats (n = 8 per group) submitted to bilateral hindlimb immobilization (IMOB), orally supplemented (for 14 days concomitantly to immobilization period) with dipeptide L-alanyl- L-glutamine (DIP-IMOB) or a solution containing the amino acids L-glutamine plus L- alanine (GLN+ALA-IMOB), both in their free form and, non-immobilized (CTRL, GLN+ALA and DIP) animals. CTRL (control group) and IMOB groups received equal volumes of water via gastric gavage in the same period. Data are reported as mean ± S.E.M. * ® p<0,05 compared to CTRL group; # p<0,05 compared to IMOB group; p<0,05 compared to GLN+ALA and DIP groups (Tukey HSD).

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