Phospho-CHUK/IKBKB (S176/177) Antibody

Code CSB-PA006829
Size US$167
Image
  • Western Blot analysis of NIH-3T3 cells using Phospho-IKKα/β (S176/177) Polyclonal Antibody
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Product Details

Uniprot No. O15111O14920
Target Names CHUK/IKBKB
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Immunogen Synthesized peptide derived from Human IKKα/β around the phosphorylation site of S176/177.
Conjugate Non-conjugated
Isotype IgG
Purification Method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form Liquid
Tested Applications WB, IHC, ELISA
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
Protocols Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

Oral glutamine supplementation attenuate inflammation and oxidative stress-mediated skeletal muscle protein content degradation in immobilized rats: Role of 70 kDa heat shock protein. Petry ER, et al,Free Radical Biology and Medicine,2019

Applications: WB
Antibody dilution factor: 1: 1000
Review: Western blot analysis of total NF-kB p65 (A), phosphorylated NF-kB p65 (B), total IKKα/β (C) and phosphorylated IKKα/β (D) protein expression in soleus muscle of Wistar rats (n = 8 per group) submitted to bilateral hindlimb immobilization (IMOB), orally supplemented (for 14 days concomitantly to immobilization period) with dipeptide L-alanyl- L-glutamine (DIP-IMOB) or a solution containing the amino acids L-glutamine plus L- alanine (GLN+ALA-IMOB), both in their free form and, non-immobilized (CTRL, GLN+ALA and DIP) animals. CTRL (control group) and IMOB groups received equal volumes of water via gastric gavage in the same period. Data are reported as mean ± S.E.M. * ® p<0,05 compared to CTRL group; # p<0,05 compared to IMOB group; p<0,05 compared to GLN+ALA and DIP groups (Tukey HSD).

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