THOC1 Antibody

Datasheet
Code CSB-PA853419ESR1HU
Size US$167Purchase it in Cusabio online store
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Uniprot No. Q96FV9
Image
  • Western blot
    All lanes: THOC1 antibody at 3μg/ml
    Lane 1: Jurkats whole cell lysate
    Lane 2: 293T whole cell lysate
    Lane 3: HepG2 whole cell lysate
    Lane 4: A549 whole cell lysate
    Lane 5: Mouse kidney tissue
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 76, 44 kDa
    Observed band size: 76 kDa

  • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA853419ESR1HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA853419ESR1HU at dilution of 1:100

  • Chromatin Immunoprecipitation 293T (1.6*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4μg anti-THOC1 or a control normal rabbit IgG. The resulting ChIP DNA was quantified tissue using real-time PCR with primers (CSB-PP853419HU) against the RHOX5 promoter.

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol
Immunogen Recombinant Human THO complex subunit 1 protein (428-657AA)
Raised in Rabbit
Species Reactivity Human, Mouse
Tested Applications ELISA, WB, IHC, ChIP; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200
Relevance Required for efficient export of polyadenylated RNA. Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. Regulates transcriptional elongation of a subset of genes. Involved in genome stability by preventing co-transcriptional R-loop formation. Participates in an apoptotic pathway which is characterized by activation of caspase-6, increases in the expression of BAK1 and BCL2L1 and activation of NF-kappa-B. This pathway does not require p53/TP53, nor does the presence of p53/TP53 affect the efficiency of cell killing. Activates a G2/M cell cycle checkpoint prior to the onset of apoptosis. Apoptosis is inhibited by association with RB1.
Form Liquid
Conjugate Non-conjugated
Storage Buffer PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method Antigen Affinity Purified
Isotype IgG
Clonality Polyclonal
Alias THO complex subunit 1 (Tho1) (Nuclear matrix protein p84) (p84N5) (hTREX84), THOC1, HPR1
Immunogen Species Human
Research Area Tags & Cell Markers
Target Names THOC1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Required for efficient export of polyadenylated RNA. Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. Regulates transcriptional elongation of a subset of genes. Involved in genome stability by preventing co-transcriptional R-loop formation.; FUNCTION
Subcellular Location Isoform 1: Nucleus speckle, Nucleus, nucleoplasm, Nucleus matrix, Cytoplasm
Tissue Specificity Ubiquitous. Expressed in various cancer cell lines. Expressed at very low levels in normal breast epithelial cells and highly expressed in breast tumors. Expression is strongly associated with an aggressive phenotype of breast tumors and expression correl
Database Links

HGNC: 19070

OMIM: 606930

KEGG: hsa:9984

STRING: 9606.ENSP00000261600

UniGene: Hs.712543

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