Recombinant Allochromatium vinosum Poly-beta-hydroxybutyrate polymerase (phbC)

Code CSB-YP338167AZP
MSDS
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Source Yeast
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Code CSB-EP338167AZP
MSDS
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Source E.coli
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Code CSB-EP338167AZP-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP338167AZP
MSDS
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Source Baculovirus
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Code CSB-MP338167AZP
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
phaC
Uniprot No.
Alternative Names
phaC; phbC; Alvin_0061Poly(3-hydroxyalkanoate) polymerase subunit PhaC; PHA polymerase; EC 2.3.1.-; PHB synthase subunit PhaC; Poly(hydroxyalkanoic acid) synthase subunit PhaC; PHA synthase subunit PhaC; Polyhydroxyalkanoic acid synthase; Poly-beta-hydroxybutyrate polymerase; PHB polymerase; Poly(3-hydroxybutyrate) polymerase
Species
Allochromatium vinosum (strain ATCC 17899 / DSM 180 / NBRC 103801 / NCIMB 10441 / D) (Chromatium vinosum)
Expression Region
1-355
Target Protein Sequence
MFPIDIRPDK LTQEMLDYSR KLGQGMENLL NAEAIDTGVS PKQAVYSEDK LVLYRYDRPE GAPEAQPVPL LIVYALVNRP YMTDIQEDRS TIKGLLATGQ DVYLIDWGYP DQADRALTLD DYINGYIDRC VDYLREAHGV DKVNLLGICQ GGAFSLMYSA LHPDKVRNLV TMVTPVDFKT PDNLLSAWVQ NVDIDLAVDT MGNIPGELLN WTFLSLKPFS LTGQKYVNMV DLLDDPDKVK NFLRMEKWIF DSPDQAGETF RQFIKDFYQN NGFLNGGVVL GGQEVDLKDI TCPVLNIFAL QDHLVPPDAS RALKGLTSSP DYTELAFPGG HIGIYVSGKA QKEVTPAIGK WLNER
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Polymerizes D(-)-3-hydroxybutyryl-CoA to create polyhydroxybutyrate (PHB) which consists of thousands of hydroxybutyrate molecules linked end to end. This subunit has catalytic activity that is enhanced 100-fold by PhaE, the non-catalytic subunit.
Subcellular Location
Cytoplasm.
Protein Families
PHA/PHB synthase family, Type III PhaC subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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