Recombinant Bovine Histone H1oo (H1FOO)

Code CSB-YP662993BO
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP662993BO
MSDS
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Source E.coli
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Code CSB-EP662993BO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP662993BO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP662993BO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
H1FOO
Uniprot No.
Alternative Names
H1FOO; H1OO; Histone H1oo; Oocyte-specific histone H1; Oocyte-specific linker histone H1
Species
Bos taurus (Bovine)
Expression Region
1-343
Target Protein Sequence
MAPGSIASSD TSSSTSSSST SSASSASAEG SSRPLGSEKP GLARGAVRAP RRHPPVLRMV LEALQAGERR RGTSVAAIKV YILQKYPTVD ALRLNHLLKQ ALATGLHRGL LIRPVNSKAK GATGSFKLVP KDKRKIPPRK TAPRMPGQAE GKDPKKPSES KKDPANTVEV KKGSRKPREE RAAPSKPGAA KKAPKKGTQT KDPEPRLGEA KKSSRRPDKA AQAPPSAGGP GGKSKVKERG SRQADTKAHR KTQPGSQSSK STVTKGENGA PLAKKKMGGK VPKEAAGEGP KAKAPVPPKG AGSKKEPGPL AGKAEASKGP RKPGIPTKSS VSKAASKKAE AEG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
May play a key role in the control of gene expression during oogenesis and early embryogenesis, presumably through the perturbation of chromatin structure. Essential for meiotic maturation of germinal vesicle-stage oocytes. The somatic type linker histone H1c is rapidly replaced by H1oo in a donor nucleus transplanted into an oocyte. The greater mobility of H1oo as compared to H1c may contribute to this rapid replacement and increased instability of the embryonic chromatin structure. The rapid replacement of H1c with H1oo may play an important role in nuclear remodeling.
Gene References into Functions
  1. H1foo is essential for bovine oocyte meiotic maturation and its overexpression stimulates the process. PMID: 24618348
  2. Results describe the characterization of linker histone H1FOO during bovine in vitro embryo development. PMID: 16470586
Subcellular Location
Cytoplasm. Nucleus. Chromosome. Note=In the 1-, 2- and 4-cell embryo, it is uniformly spread within the cytoplasm, while it is concentrated onto the chromatin in the nucleus. In the 8- to 16-cell embryo, its presence diminishes in the cytoplasm, although it is still strongly expressed in the nucleus.
Protein Families
Histone H1/H5 family
Tissue Specificity
Oocyte (at protein level).
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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