Recombinant Escherichia coli UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)

Code CSB-YP358910ENV
MSDS
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Source Yeast
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Code CSB-EP358910ENV-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP358910ENV
MSDS
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Source Baculovirus
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Code CSB-MP358910ENV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
lpxC
Uniprot No.
Alternative Names
lpxC; asmB; envA; b0096; JW0094; UDP-3-O-acyl-N-acetylglucosamine deacetylase; UDP-3-O-acyl-GlcNAc deacetylase; EC 3.5.1.108; Protein EnvA; UDP-3-O-[R-3-hydroxymyristoyl]-N-acetylglucosamine deacetylase
Species
Escherichia coli (strain K12)
Expression Region
1-305
Target Protein Sequence
MIKQRTLKRI VQATGVGLHT GKKVTLTLRP APANTGVIYR RTDLNPPVDF PADAKSVRDT MLCTCLVNEH DVRISTVEHL NAALAGLGID NIVIEVNAPE IPIMDGSAAP FVYLLLDAGI DELNCAKKFV RIKETVRVED GDKWAEFKPY NGFSLDFTID FNHPAIDSSN QRYAMNFSAD AFMRQISRAR TFGFMRDIEY LQSRGLCLGG SFDCAIVVDD YRVLNEDGLR FEDEFVRHKM LDAIGDLFMC GHNIIGAFTA YKSGHALNNK LLQAVLAKQE AWEYVTFQDD AELPLAFKAP SAVLA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes the hydrolysis of UDP-3-O-myristoyl-N-acetylglucosamine to form UDP-3-O-myristoylglucosamine and acetate, the committed step in lipid A biosynthesis.
Gene References into Functions
  1. analysis of mutants resistant to LpxC inhibitors by rebalancing cellular homeostasis PMID: 23316051
  2. the active site metal ion in UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase (LpxC) switches between Fe(II) and Zn(II) depending on cellular conditions PMID: 20709752
  3. The C-terminus of LpxC contains a signal sequence necessary for FtsH-dependent degradation. PMID: 16420369
  4. potency of LpxC inhibitors in vitro can be altered by assay conditions and development of inhibitorsand that the metal ion status of LpxC in vivo may influence the effectiveness of LpxC inhibitors as antibiotics. PMID: 17144651
  5. Functions of LpxC active site residues have been classified on the basis of changes in steady-state turnover and product binding affinity; side chains are identified that enhance and destabilize product affinity as well as enhance catalytic efficiency. PMID: 17176046
  6. A C-terminal tail of about 20 amino acids length is required for proteolysis of LpxC by FtsH PMID: 17651755

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Protein Families
LpxC family
Database Links
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