Name: Recombinant Escherichia coli UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
Brand: CUSABIO
SKU: CSB-EP358910ENV

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

lpxC

Uniprot No.

P0A725

Research Area

others

Alternative Names

lpxC; asmB; envA; b0096; JW0094; UDP-3-O-acyl-N-acetylglucosamine deacetylase; UDP-3-O-acyl-GlcNAc deacetylase; EC 3.5.1.108; Protein EnvA; UDP-3-O-[R-3-hydroxymyristoyl]-N-acetylglucosamine deacetylase

Species

Escherichia coli (strain K12)

Source

E.coli

Expression Region

1-305aa

Target Protein Sequence

MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDLNPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIPIMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTIDFNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVVDDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVLAKQEAWEYVTFQDDAELPLAFKAPSAVLA
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

38.0kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Tris-based buffer,50% glycerol

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

Is this protein have biological activity?

Very nice to receive your inquiry. We could provide two kinds of tags for this protein. The product details are confirmed as below.
Code: CSB-EP358910ENVa2
Name: Recombinant Escherichia coli UDP-3-O-acyl-N-acetylglucosamine deacetylase(lpxC)
Expression Region: 1-305aa, full length
Tag Info: N-terminal 6xHis-SUMO-tag
Expression Sequence:

Name: Recombinant Escherichia coli UDP-3-O-acyl-N-acetylglucosamine deacetylase(lpxC)
Expression Region: 1-305aa, full length
Tag Info: N-terminal 6xHis-tagged
Expression Sequence:

Is this product RPC20462/ CSB-EP358910 Recombinant Escherichia coli UDP-3-O-acyl-N-acetylglucosamine deacetylase(lpxC) storaged in buffer contained this?

Very nice to receive your inquiry.
Name: Recombinant Escherichia coli UDP-3-O-acyl-N-acetylglucosamine deacetylase(lpxC)
Expression Region: 1-305aa, full length
Tag Info: N-terminal 6xHis-SUMO-tag
Expression Sequence:

For this protein, we have in-stock proteins delivered in different forms, as below.
1. Delivery Form: Liquid; Storage Buffer: 10 mM Tris-HCl,1 mM EDTA, pH 8.0, 50% glycerole; Lot: 03600; Inventory Amount: 1.34mg
2. Delivery Form: Lyophilized Powder; Buffer before Lyophilization: Lyophilized from a 0.2 μm filtered 10 mM Tris-HCl,1 mM EDTA, pH8.0; Inventory Amount: Sufficient

Target Background

Function

Catalyzes the hydrolysis of UDP-3-O-myristoyl-N-acetylglucosamine to form UDP-3-O-myristoylglucosamine and acetate, the committed step in lipid A biosynthesis.

Gene References into Functions

analysis of mutants resistant to LpxC inhibitors by rebalancing cellular homeostasis PMID: 23316051
the active site metal ion in UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase (LpxC) switches between Fe(II) and Zn(II) depending on cellular conditions PMID: 20709752
The C-terminus of LpxC contains a signal sequence necessary for FtsH-dependent degradation. PMID: 16420369
potency of LpxC inhibitors in vitro can be altered by assay conditions and development of inhibitorsand that the metal ion status of LpxC in vivo may influence the effectiveness of LpxC inhibitors as antibiotics. PMID: 17144651
Functions of LpxC active site residues have been classified on the basis of changes in steady-state turnover and product binding affinity; side chains are identified that enhance and destabilize product affinity as well as enhance catalytic efficiency. PMID: 17176046
A C-terminal tail of about 20 amino acids length is required for proteolysis of LpxC by FtsH PMID: 17651755

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Protein Families

LpxC family

Database Links

KEGG: ecj:JW0094

STRING: 316385.ECDH10B_0078

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