Recombinant Escherichia coli UDP-3-O-acyl-N-acetylglucosamine deacetylase(lpxC)

In Stock
Code CSB-EP358910ENVa2
Size US$2466
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP358910ENVa2 could indicate that this peptide derived from E.coli-expressed Escherichia coli (strain K12) lpxC.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP358910ENVa2 could indicate that this peptide derived from E.coli-expressed Escherichia coli (strain K12) lpxC.
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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names lpxC
Uniprot No. P0A725
Research Area others
Alternative Names lpxC; asmB; envA; b0096; JW0094; UDP-3-O-acyl-N-acetylglucosamine deacetylase; UDP-3-O-acyl-GlcNAc deacetylase; EC; Protein EnvA; UDP-3-O-[R-3-hydroxymyristoyl]-N-acetylglucosamine deacetylase
Species Escherichia coli (strain K12)
Source E.coli
Expression Region 1-305aa
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 49.8kDa
Protein Length Full Length
Tag Info N-terminal 6xHis-SUMO-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Catalyzes the hydrolysis of UDP-3-O-myristoyl-N-acetylglucosamine to form UDP-3-O-myristoylglucosamine and acetate, the committed step in lipid A biosynthesis.
Gene References into Functions
  1. analysis of mutants resistant to LpxC inhibitors by rebalancing cellular homeostasis PMID: 23316051
  2. the active site metal ion in UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase (LpxC) switches between Fe(II) and Zn(II) depending on cellular conditions PMID: 20709752
  3. The C-terminus of LpxC contains a signal sequence necessary for FtsH-dependent degradation. PMID: 16420369
  4. potency of LpxC inhibitors in vitro can be altered by assay conditions and development of inhibitorsand that the metal ion status of LpxC in vivo may influence the effectiveness of LpxC inhibitors as antibiotics. PMID: 17144651
  5. Functions of LpxC active site residues have been classified on the basis of changes in steady-state turnover and product binding affinity; side chains are identified that enhance and destabilize product affinity as well as enhance catalytic efficiency. PMID: 17176046
  6. A C-terminal tail of about 20 amino acids length is required for proteolysis of LpxC by FtsH PMID: 17651755

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Protein Families LpxC family
Database Links

KEGG: ecj:JW0094

STRING: 316385.ECDH10B_0078


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