Name: Recombinant Halobacterium salinarum Cobalamin import system permease protein BtuC(btuC),partial
Brand: CUSABIO
SKU: 532244HTL

Product Details

Purity	>85% (SDS-PAGE)
Target Names	btuC
Uniprot No.	B0R5G3
Alternative Names	btuC; OE_2952F; Cobalamin import system permease protein BtuC
Species	Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
Protein Length	Partial
Tag Info	The following tags are available. N-terminal His-tagged Tag-Free The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form	Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization	Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs	Protein FAQs
Storage Condition	Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time	Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time. Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet	Please contact us to get it.

Customer Reviews and Q&A

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■ Q&A

we are interested in purchasing it in either detergent or lipid (liposome, nanodisc etc.) environment.
You guys usually use detergents, right? What detergent and usually what quantity?

Thanks for your interest in our product. Actually we don't use any detergents during the soluble expression, and all of our proteins are expressed in soluble form. So we don't use detergents.
But sometimes we will use the detergents during the expression of the transmembrane proteins, there are many types of detergents,
such as DDM, LDAO, CHAPS, etc., we can prepare according to the specific choice of you. Pls remark your requirement when placing the order.

1.I plans to use these proteins in AFM imaging experiments. Do you think aseptic processing and/or endotoxin removal are necessary for these experiments?
2.Can you advise whether or not these proteins are suitable for lyophilization?
3.CSB-CF532244HTL(A4), can the protein have N-terminal His-tagged, and dissolved in LDAO?

Thanks for your interest in our product,1.Our technical team think aseptic processing or endotoxin removal are not necessary for these experiments.
2. Glycerol will be added into the buffer for liquid protein. If the glycerol will affect the customer's experiments, lyophilized protein is suitable.
3. We will give a priority to requested tag, but cannot 100% guaranteed, success rate is 95.25%. If it fails in expresssing, we will use other type of tags. CSB-CF532244HTL(A4) can dissolved in LDAO. Please remark when you place an order, if the customer request LDAO.

Target Background

Function	Required for corrinoid utilization. Probably part of the ABC transporter complex BtuCDF involved in cobalamin (vitamin B12) import. Probably involved in the translocation of the substrate across the membrane.
Subcellular Location	Cell membrane; Multi-pass membrane protein.
Protein Families	Binding-protein-dependent transport system permease family, FecCD subfamily
Database Links	KEGG: hsl:OE_2952F

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Recombinant Halobacterium salinarum Cobalamin import system permease protein BtuC(btuC),partial

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Target Background

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